- P-ISSN 1225-0163
- E-ISSN 2288-8985
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Improved HPLC-UV method for determination of five synthetic dyes in Typha orientalis
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2021, v.34 no.4, pp.160-171
https://doi.org/10.5806/AST.2021.34.4.160
(National Institute of Food and Drug Safety Evaluation)
(Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation)
(National Institute of Food and Drug Safety Evaluation)
(Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation)
(Department of Food Science and Technology, Chung-Ang University)
(Division of Food and Animal Science, Chungbuk National University)
(Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation)
(Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation)
(Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation)
(Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation)
(Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation)
(National Institute of Food and Drug Safety Evaluation)
(Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation)
(Department of Food Science and Technology, Chung-Ang University)
(Division of Food and Animal Science, Chungbuk National University)
(Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation)
(Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation)
(Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation)
(Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation)
Kyung,
Y.
K.
, Eun,
Y.
C.
, Se,
H.
J.
, Ock,
j.
P.
, Chan,
L.
, Huijin,
H.
, She-Wook,
O.
, Chulhyun,
L.
, Juhye,
K.
, &
Sooyeul,
C.
(2021). Improved HPLC-UV method for determination of five synthetic dyes in Typha orientalis. Analytical Science and Technology, 34(4), 160-171, https://doi.org/10.5806/AST.2021.34.4.160
- Downloaded
- Viewed
Abstract
Synthetic azo dyes are used extensively in herbal medicines to render the medicines more visually attractive to consumers. This study developed and validated a rapid high-performance liquid chromatography (HPLC) method to determine whether synthetic colorants such as Tartrazine, Auramine O, Metanil yellow, Sunset yellow, and Orange II are used extensively in Typha orientalis. To increase the recovery of the synthetic dyes, this method employed containing 50 mM ammonium acetate in 70 % methanol at first extraction and 100 mM HCl in 70 % methanol at second extraction. Five synthetic pigments in Typha orientalis were separated by gradient elution with a mobile phase consisting of acetonitrile and 50 mM ammonium acetate in distilled water at ultra-violet (UV) detection 428 nm or 500 nm. Additionally, this study established the liquid chromatography tandem mass spectrometry (LC-MS/MS) method to confirm positive samples suspected by HPLC results. The HPLC-UV method had good linearity, indicating r2> 0.999. The recoveries of the samples spiked with three different concentration ranged from 73.8~91.5 %, and relative standard deviation values indicated 0.2~5.2 %. The established LC-MS/MS could successfully identify the synthetic pigments in herbal medicine samples. The study demonstrates that Typha orientalis adulterated by yellowish synthetic dyes can be successfully distinguished when using the HPLC-UV method.
- keywords
- herbal medicines, synthetic dyes, Typha orientalis, HPLC, LC-MS/MS
Reference
1
1. B. Esen, T. Oymak and E. Dural, Int. J. Sci. Eng. Res., 9(8), 72-76 (2018).
2
2. M. Xu, B. Huang, F. Gao, C. Zhai, Y. Yang, L. Li, W. Wang and L. Shi, Front. Pharmcol., 10, Article 1446, 1-8 (2019).
3
3. K. Rovina, S. Siddiquee and S. M. Shaarani, Crit. Rev. Anal. Chem., 47(4), 309-324 (2017).
4
4. K. S. Rowe and K. J. Rowe, J. Pediatrics, 125(5), 691-698 (1994).
5
5. Y. S. Al-Degs, Food Chem., 117(3), 485-490 (2009).
6
6. T. Nagaraja and T. Desiraju, Food Chem. Toxicol., 31(1), 41-44 (1993).
7
7. I. S. Khan, M. N. Ali, R. Hamid and S. A. Ganie, Toxicol. Rep., 7, 370-375 (2020).
8
8. M. Solís, A. Solís, H. I. Pérez, N. Manjarrez and M. Flores, Process Biochem., 47(12), 1723-1748 (2012).
9
9. K. T. Chung, G. E. Fulk and M. Egan, Appl. Environ. Microbiol., 35(3), 558-562 (1978).
10
10. R. Anliker, Richardson M., Ed., The Royal Society of Chemistry. London, UK. 166-187 (1986).
11
11. J. C. Tung, W. C. Huang, J. C. Yang, G.Y. Chen, C. C. Fan, Y. C. Chien, P. S. Lin, S. C. C. Lung and W. C. Chang, Environ. Toxicol., 32(11), 2379-2391 (2017).
12
12. S. Parodi, L. Santi, P. Russo, A. Albini, D. Vecchio, M. Pala, L. Ottaggio and A. Carbone, J. Toxicol. Environ. Health, 9(5-6), 941-952 (1982).
13
13. T. N. T. Kim, T. T. Bui, A. T. Pham, V. T. Duong and T. H. G Le, J. Anal. Methods Chem., 2019, Article ID 8639528 (2019).
14
14. T. I. Tikhomirova, G. R. Ramazanova and V. V. Apyari, Food Chem., 221, 351-355 (2017).
15
15. G. Karanikolopoulos, A. Gerakis, K. Papadopoulou and I. Mastrantoni, Food Chem., 177, 197-203 (2015).
16
16. F. Martin, J. M. Oberson, M. Meschiari and C. Munari, Food Chem., 197, 1249-1255 (2016).
Other articles from this issue
- Enantioselective electrophoretic behavior of lipoic acid in single and dual cyclodextrin systems
- Optimization of an extraction method for the simultaneous quantification of six active compounds in the aril part of Orostachys japonicus using HPLC–UV
- Improved HPLC-UV method for determination of five synthetic dyes in Typha orientalis
- A fragmentation database of soyasaponins by liquid chromatography with a photodiode array detector and tandem mass spectrometry
- Effective determination of nicotine enantiomers from e-liquids and biological fluids by high performance liquid chromatography (HPLC) using dispersive liquid-liquid microextraction (DLLME)
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.