- P-ISSN 1225-0163
- E-ISSN 2288-8985
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
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Effective determination of nicotine enantiomers from e-liquids and biological fluids by high performance liquid chromatography (HPLC) using dispersive liquid-liquid microextraction (DLLME)
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2021, v.34 no.4, pp.180-190
https://doi.org/10.5806/AST.2021.34.4.180
(Kyonggi University)
(Department of Chemistry, Kyonggi University)
(Department of Chemistry, Kyonggi University)
Seunghoon,
S.
, &
Seung-Woon,
M.
(2021). Effective determination of nicotine enantiomers from e-liquids and biological fluids by high performance liquid chromatography (HPLC) using dispersive liquid-liquid microextraction (DLLME). Analytical Science and Technology, 34(4), 180-190, https://doi.org/10.5806/AST.2021.34.4.180
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Abstract
This study compared the efficacy of chiral GC and chiral HPLC for the analysis of nicotine. To develop a suitable dispersive liquid-liquid microextraction (DLLME) method, the following parameters were optimized: pH, extraction solvent, dispersive solvent, type and quantity of salt, and laboratory temperature. The validation of the method was carried out by the established HPLC method. The LODs were 0.11 μg/mL and 0.17 μg/mL for the (S)- and (R)- enantiomers, respectively. The LOQs were 0.30 μg/mL and 0.44 μg/mL, respectively. The optimal calibration range was between 0.30-18 μg/mL and 0.44-4.40 μg/mL, respectively, and the correlation coefficient (r2) was 0.9978-0.9996. The intra-day accuracy was 79.9-110.6 %, and the intra-day precision was 1.3-12.0 %. The inter-day accuracy was 87.8-108.0 %, and the inter-day precision was 4.0-12.8 %. E-liquid and biological fluids (urine and saliva) were analyzed using the established method.
- keywords
- nicotine enantiomers, DLLME, HPLC, GC/MS, e-liquid, urine, saliva
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