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- E-ISSN 2288-8985
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Effective determination of nicotine enantiomers from e-liquids and biological fluids by high performance liquid chromatography (HPLC) using dispersive liquid-liquid microextraction (DLLME)
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2021, v.34 no.4, pp.180-190
https://doi.org/10.5806/AST.2021.34.4.180
(Kyonggi University)
(Department of Chemistry, Kyonggi University)
(Department of Chemistry, Kyonggi University)
Seunghoon,
S.
, &
Seung-Woon,
M.
(2021). Effective determination of nicotine enantiomers from e-liquids and biological fluids by high performance liquid chromatography (HPLC) using dispersive liquid-liquid microextraction (DLLME). Analytical Science and Technology, 34(4), 180-190, https://doi.org/10.5806/AST.2021.34.4.180
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Abstract
This study compared the efficacy of chiral GC and chiral HPLC for the analysis of nicotine. To develop a suitable dispersive liquid-liquid microextraction (DLLME) method, the following parameters were optimized: pH, extraction solvent, dispersive solvent, type and quantity of salt, and laboratory temperature. The validation of the method was carried out by the established HPLC method. The LODs were 0.11 μg/mL and 0.17 μg/mL for the (S)- and (R)- enantiomers, respectively. The LOQs were 0.30 μg/mL and 0.44 μg/mL, respectively. The optimal calibration range was between 0.30-18 μg/mL and 0.44-4.40 μg/mL, respectively, and the correlation coefficient (r2) was 0.9978-0.9996. The intra-day accuracy was 79.9-110.6 %, and the intra-day precision was 1.3-12.0 %. The inter-day accuracy was 87.8-108.0 %, and the inter-day precision was 4.0-12.8 %. E-liquid and biological fluids (urine and saliva) were analyzed using the established method.
- keywords
- nicotine enantiomers, DLLME, HPLC, GC/MS, e-liquid, urine, saliva
Reference
1
1. E. Mehmeti, T. Kilic, C. Laur and S. Carrara, Microchem. J., 158, Article 105155 (2020).
2
2. M. D. Aceto, B. R. Martin, I. M. Uwaydah, E. L. May, L. S. Harris, C. Izazola-Conde, W. L. Dewey, T. J. Bradshaw and W. C. Vincek, J. Med. Chem., 22(2), 174-177 (1979).
3
3. D. Yildiz, N. Ercal and D. W. Armstrong, J. Toxicol., 130, 155-165 (1998).
4
4. E. W. Willems, B. Rambali, W. Vleeming, A. Opperhuizen and J. G. C. van Amsterdam, Food Chem. Toxicol., 44(5), 678-688 (2006).
5
5. D. W. Armstrong, X. Wang and N. Ercal, Chirality, 10, 587-591 (1998).
6
6. A. McQueen, S. Tower and W. Summer, Nicotine Tob. Res., 13(9), 860-867 (2011).
7
7. Z. Xiao-Huan, W. Qiu-Hua, Z. Mei-Yue, X. Guo-Hong and W. Zhi, Chin. J. Anal. Chem., 37(2), 161-168 (2009).
8
8. A. Zgola-Grzeskowiak and T. Grzeskowiak, Trends Anal. Chem., 30(9) 1382-1399 (2011).
9
9. M. Rezaee, Y. Assadi, M. R. Milani Hosseini, E. Aghaee, F. Ahmadi and S. Berijani, J. Chromatogr. A, 1116(1-2), 1-9 (2006).
10
10. M. Bansal, M. Sharma, C. Bullen and D. Svirskis, Int. J. Environ. Res. Public Health, 15(8), 1737-1747 (2018).
11
11. H. Zhang, Y. Pang, Y. Luo, X. Li, H. Chen, S. Han, X. Jiang, F. Zhu, H. Hou and Q. Hu, Chirality, 30(7), 923-931 (2018).
12
12. H. Ji, Y. Wu, F. Fannin and L. Bush, Heliyon, 5(5), e01719(2019).
13
13. M. Piller, G. Gilch, G. Scherer and M. Scherer, J. Chromatography, 951-952, 7-15 (2014).
14
14. M. L. Trehy, W. Ye, M. E. Hadwiger, T. W. Moore, J. F. Allgire, J. T. Woodruff, S. S. Ahadi, J. C. Black and B. J. Westenberger, J. Liq. Chromatogr. Relat. Technol., 34(14), 1442-1458 (2011).
15
15. G. D. Byrd, R. A. Davis and M. W. Ogden, J. Chromatogr. Sci., 43, 133-140 (2005).
16
16. Y. Tang, W. L. Zielinski and H. M. Bigott, Chirality, 10, 364-369 (1998).
17
17. V. V. Gholap, R. S. Heyder, L. Kosmider and M. S. Halquist, J. Anal. Methods Chem., 2020(1), 1-12 (2020).
18
18. D. Demetriou, K. Rustemeier, P. Voncken and G. Schepers, Chirality, 5, 300-302 (1993).
19
19. L. Q. Sheng, L. Ding, H. W. Tong, G. P. Yong, X. Z. Zhou and S. M. Liu, Chromatographia, 62, 63-68 (2005).
20
20. P. Clayton, A. Lu and L. Bishop, Chirality, 22(4), 442-446 (2010).
21
21. S. C. Moldoveanu, A. G. Hudson and A. Harrison, Beitr. Tabakforsch. Int., 27(7), 145-153 (2017).
22
22. T. Pagano, A. G. Difrancesco, S. B. Smith, J. George, G. Wink, I. Rahman and R. J. Robinson, Nicotine Tob. Res., 18(5), 700-707 (2016).
23
23. T. Perfetti and A. Rodgman, Beitr. Tab. Int., 24(5), 215-232 (2011).
24
24. J. Aszyk, M. K. Wozniak, P. Kubica, A. Kot-Wasik, J. Namiesnik and A. Wasik, J. Chromatogr. A, 1517, 156-164 (2017).
25
25. Enforcement Regulations of the Bioethics and Safety Act, No. 773, Dec. 31 (2020), Rep. of Korea.
26
26. S. S. Hecht, S. G. Carmella, M. Chen, J. F. Dor Koch, A. T. Miller, S. E. Murphy, J. A. Jensen, C. L. Zimmerman and D. K. Hatsukami, Cancer Res., 59, 590-596 (1999).
27
27. S. Seidi, M. Rezazadeh and R. Alizadeh, Bioanalysis, 11(2), 119-148 (2019).
28
28. Y. F. Zhang, J. Zheng, L. Zheng and Z. R. Zhou, Biosurf. Biotribol., 2(3), 95-101 (2016).
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