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UV-spectrophotometric and electrophoretic analysis composition of protein and the content of sugars of young Ricinus communis L. by cold treatment
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1995, v.8 no.2, pp.181-186
Cho,
B., Park,
S. Y., &
Park,
M.
(1995). UV-spectrophotometric and electrophoretic analysis composition of protein and the content of sugars of young Ricinus communis L. by cold treatment. Analytical Science and Technology, 8(2), 181-186.
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Abstract
The contents of sucrose and reducing sugars of cotyledons and hypocotyls of Ricinus communis L were increased slightly during cold treatment at <TEX>$4^{\circ}C$</TEX>. The concentration of total amino acids was increased continuosly during cold treatment. But the contents of hydrophilic amino acids, Asp/Asn, Glu/Gln, Thr, Ser, Ala and cationic amino acids, Arg and Lys were varied dramatically with the cold treated time. The cold treatment induced 24, 52, 54, 55, 56 and 73.5kD of proteins in cotyledons and 55, 56 and 73.5kD of proteins in hypocotyls. 24, 42, 49 and 52kD of proteins in cotyledons and hypocotyls were boiling stable. They were not denatrated by boiling at <TEX>$100^{\circ}C$</TEX>.
- keywords
- amino acids, sugars, proteins, boiling stable
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