바로가기메뉴

본문 바로가기 주메뉴 바로가기

logo

Cadmium-Induced Phytotoxicity in Tomato Seedlings Due to the Accumulation of H2O2 That Results from the Reduced Activities of H2O2 Detoxifying Enzymes

Journal of Ecology and Environment / Journal of Ecology and Environment, (P)2287-8327; (E)2288-1220
2004, v.27 no.2, pp.107-114

  • Downloaded
  • Viewed

Abstract

Tomato (Lycopersicon esculentum) seedlings exposed to various concentrations of CdCl2 (0-100μM) in the nutrient solution for up to 9 days were analyzed with the seedling growth, H2O2 production, glutathione levels and activity changes of enzymes related to H2O2 removal. The growth of seedlings was inhibited with over 50 μM Cd, whereas the levels of H2O2 and glutathione were enhanced with Cd exposure level and time. Meanwhile, Cd exposure increased the activities of catalase (CAT) and glutathione reductase (GR) but decreased the activities of dehydroascorbate acid reductase (DHAR) and ascorbate peroxidase (APX) in both leaves and roots. These results suggest that the altered activities of antioxidant enzymes particularly involved in the H2O2 removal and the subsequent H2O2 accumulation could induce the Cd-induced phytotoxicity.

keywords
Cadmium, Hydrogen peroxide, Lycopersicon esculentum

Reference

1.

(1989) Biosynthesis and antioxidant function of glutathionein plants,

2.

(ascorbateperoxidase-ahydrogenperoxidescavengingenzymeinplants) Physiol,

3.

(1994) Heavy metalaccumulation and tolerance in British populations of themetallophyte Thlaspi caerulescens J,

4.

(1990) Plant water relations asaffected by heavy metal stress,

5.

(2003) Organic acid complexation,heavy metal distribution and the effect of ATPase inhibition inhairy roots of hyperaccumulator plant species,

6.

(1976) A rapid and sensitive method for the quantitationof microgram quantity of protein utilizing the principleof protein-dye binding,

7.

(1990196-204) γ-glutamylcysteine and glutathione contents of spinachleaves as affected by darkness and application of excesssulfur Glutathione accumulation in detached leaves exposedto H2S in the absence of light is stimulated by the supply ofglycine to the petiole,

8.

(1991) Effect of aluminium on lipid peroxidation catalase and peroxidase activitiesin root tips of soybean, Glycine max

9.

(1955) Assay of catalase and peroxidases,

10.

(1985) Inhibition of photosynthesisby metals,

11.

Cho, U.H. , (2000) Mercury-induced oxidative stress intomato seedlings,

12.

Chaoui, A, (1997) .Cadmium and zinc induction of lipid peroxidation and effectson antioxidant enzyme activities in bean (Phaseolus vulgarisL.). ,

13.

De Vos, (1991) Increased resistance to copper-induced damage ofthe root cell plasmalemma in copper-tolerant Silene cucubalus,

14.

De Vos, (1992) Glutathione depletion due to copper-induced phytochelatin synthesiscauses oxidative stress in Sulene cucubalus,

15.

De Vos, (1993) Effect of copper on fatty acid compositionand peroxidation of lipids in the roots of copper-tolerant and-sensitive Silene cucubalus,

16.

Dixit, V, (2001) Differential antioxidativeresponses to cadmium in roots and leaves of pea (Pisum sativumL. cv. Azad). ,

17.

Ebbs, S, (2002) Phytochelatinsynthesis is not responsible for Cd tolerance in the Zn/Cdhyperaccumulatior Thlapsi (J&C. Presl). ,

18.

Ernst, W.H.O, (2000) Combinationtoxicology of metal-enriched soils: physiological responsesof a Zn- and Cd-resistant ecotype of Silene vulgaris on polymetallic sols,

19.

Fadzilla, N.M, (1997) Salinity, oxidativestress and antioxidant responses in shoot cultures of rice,

20.

Foyer, (1994) Photooxidativestress in plants,

21.

Grill, (1985) theprincipal heavy-metal complexing peptites of higher plants,

22.

Hegedus, A., S. Erdei , (2001) Comparative studiesof H2O2 detoxifying enzymes in green and greening barleyseedlings under cadmium stress,

23.

Hoagland, (1938) The water culture method forgrowing plants without soil,

24.

Ianelli, M.A, (2002) Antioxidant response to cadmium in Phragmites australisplants,

25.

Karabal, E, (2003) Antioxidant responsesof tolerant and sensitive barley cultivars to boron toxicity,

26.

Kessler, A. , (1995) The mechanism of the stimulationof state 4 respirartion by cadmium in potato tuber (Solanumtuberosum) mitochondria,

27.

Kupper, H, (2004) Tissue- and age-dependent differences in the complexationof cadmium and zinc in the cadmium/zinc hyperaccumulatorThlaspi caerulescens (Ganges Ecotype) revealedby X-ray absorption spectroscopy,

28.

Lee, S, (2003) Overexpression of Arabidopsis phytochelatinsynthase paradoxically leads to hypersensitivity to cadmiumstress,

29.

(19932001-2012) Response of leguminosae to cadmium exposure,

30.

(2000) Phytochelatins and metaltolerance. In S.B. Agrawal and M. Agrawal (eds.), EnvironmentalPollution and Plant Responses, CRC Press

31.

(1996) Synthesis of glutathione in leaves of transgenicpoplar (Populus tremula × P. alba) overexpressing γ-glutamylcysteine synthetase,

32.

(1998) Ascorbate and glutathione: Keepingactive oxygen under control,

33.

(1998a) Glutathione: Biosynthesis, metabolismand relationship to stress tolerance explored in transformedplants,

34.

(1998b) Manipulationof glutathione and amino acid biosynthesis in thechloroplast,

35.

(1997) Cadmium- and copper-induced changes in tomato membranelipids,

36.

(1971) Complex formation of zinc andcadmium with glutathione,

37.

(2000) Overexpressionof glutathione reductase in Brassica juncea: effectson cadmium accumulation and tolerance,

38.

(1994) Evidence for chilling-induced oxidative stress in maize seedlingsand a regulatory role for hydrogen peroxide,

39.

(1995) Amelioration ofozone-induced oxidative damage in wheat plants grown underhigh carbon dioxide. ,

40.

(1987) Changes in glutathione content of maize seedlingsexposed to cadmium,

41.

(1995) Phytochelatins and related peptides: Structure,biosynthesis, and function,

42.

(1992) Effect of cadmium on γ-glutamylcysteine synthesis in maize seedlings,

43.

(1999) Responses to cadmiumin higher plants,

44.

(chloroplastglutathionereductase) Plant Physiol,

45.

(1987) Phytochelatin synthesis and glutathione levels in response toheavy metals in tomato cells,

46.

(1995) Regulation of glutathionesynthesis in suspension cultures of parsley and tobacco,

47.

(2001) Cadmium-induced changes in antioxodative systems, hydrogenperoxide content, and differentiation in Scots Pine roots,

48.

(1988) Asssay of glutathionereductase in crude tissue homogenates using 5,

49.

(1992) Phytoxicityof cadmium ions on germinating seedlings of mungbean involvement of lipid peroxides inchlorophyll degradation,

50.

(1996) Relationship betweencadmium, glutathione and cadmium-binding peptides (phytochelatins)in leaves of intact tobacco seedlings,

51.

(1996) Oxidative damage anddefense mechanisms in primary leaves of Phaseolus vulgaris asa result of root assimilation of toxic amounts of copper,

52.

(1997) Zn phytotoxicity inducesoxidative stress in primary leaves of Phaseolus vulgaris,

53.

(1987) evidence for the role of singlet oxygen and endogenousantioxidants,

54.

(2001) The biological functions of glutathione revisited in Arabidopsistransgenic plants with altered glutathione levels,

55.

(1999) Overexpression of glutathione synthetase in Indian mustardenhances cadmium accumulation and tolerance,

Journal of Ecology and Environment