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ISSN : 2287-8327

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Vol.27 No.5
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Annual Population Variation and Identification of Antibiotic-Resistent Bacteria in the Lower Lake Geumgang

Journal of Ecology and Environment / Journal of Ecology and Environment, (P)2287-8327; (E)2288-1220
2004, v.27 no.5, pp.283-289





& (2004). Annual Population Variation and Identification of Antibiotic-Resistent Bacteria in the Lower Lake Geumgang. Journal of Ecology and Environment, 27(5), 283-289.
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Abstract

This study was conducted to evaluate the annual population variation and identification of antibiotic-resistant bacteria in the lower artificial Lake Geumgang from January to December, 2002. Samples were taken from the surface waters at 3 stations near the estuarine barrage. The results were as follows; the population densities of heterotrophic bacteria varied from 4.1 ± 1.0 × 102 to 6.7 ± 1.1 × 103 cfu ml-1 during the investigation periods. The population densities of antibiotic-resistant bacteria ranged from 1.5 ± 0.7 × 10 to 4.3 ± 0.3 × 103 cfu ml-1 for ampicillin; from 0 to 6.4 ± 0.4 × 102 cfu ml-1 for chloramphenicol; from 0 to 2.8 ± 0.3 × 103 cfu ml-1 for gentamicin; from 0 to 4.5 ± 1.0 × 103 cfu ml-1 for kanamycin; and from 1.0 ± 0.4 × 10 to 2.3 ± 0.5 × 103 cfu ml-1 for streptomycin, respectively. Of the sixty isolates, 90% were Gram negative. Dominant genera by 16S rDNA analysis were identified Aeromonas spp. (14 strains), Bacillus spp. (6strains), Enterobacter spp. (4 strains), and Stenotrophomonas spp. (6 strains). These strains were clustered into 12 groups based on relatedness by average linkage method. Of the 60 isolates, 85% had the resistance to ampicilin and 32% were shown resistance to more than 2 kinds of antibiotics.

keywords
금강, 종속영양세균, 항생제내성세균, 16S rDNA 분석, Antibiotic-resistant bacteria, Geum River, Heterotrophic bacteria, 16S rDNA analysis

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