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ISSN : 2287-8327

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Vol.29 No.2
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Identification of Ectomycorrhizal Fungi from Pinus densiflora Seedlings at an Abandoned Coal Mining Spoils

Journal of Ecology and Environment / Journal of Ecology and Environment, (P)2287-8327; (E)2288-1220
2006, v.29 no.2, pp.143-149





& (2006). Identification of Ectomycorrhizal Fungi from Pinus densiflora Seedlings at an Abandoned Coal Mining Spoils. Journal of Ecology and Environment, 29(2), 143-149.
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Abstract

This study was conducted to identify native ectomycorhizal (ECM) fungi colonizing Pinus densiflorafor revegetation of abandoned coal mines in Korea. Sedlings of P. densiflora growing on coal mining spoils were extracted from each root tip for a seedling for molecular identification. A PCR primer pair specific to fungi, ITS1F and ITS4, was used to amplify fungal DNA. Restriction enzymes, AluI and HinfI were used for restriction fragment length polymorphism (RFLP). Combined with RFLP profiles and sequence analysis, total twenty one taxa were identified from the ECM root tips. Basidiomycetous fungi including Thelephoraceae, Pezizales, Laccaria, Pisolithus and Ascomycetous fungi including ericoid mycorrhizal fungi were identified from this study. Results showed that the most frequently found in the study sites was a species in Thelephoraceae. A possible P. densiflora was discussed.

keywords
Ectomycorrhiza, Ericoid mycorrhiza, ITS, Mine spoils, Revegetation, RFLPPark, Sang-Hyeon et al. J. Ecol. Field Biol. 29 (2) 144pine seedlings with ectomycorrhizal (ECM) fungi improved seedling growth and establishment in these sites. An ECM fungal s, Ectomycorrhiza, Ericoid mycorrhiza, ITS, Mine spoils, Revegetation, RFLPPark, Sang-Hyeon et al. J. Ecol. Field Biol. 29 (2) 144pine seedlings with ectomycorrhizal (ECM) fungi improved seedling growth and establishment in these sites. An ECM fungal s

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