- KOREAN
- P-ISSN2287-8327
- E-ISSN2288-1220
- SCOPUS, KCI
ISSN : 2287-8327
Effects of Fertilization on Physiological Parameters in American Sycamore (Platanus occidentalis) during Ozone Stress and Recovery Phase
Abstract
American sycamore seedlings were grown in chambers with two different ozone concentrations (O₃-free air and air with additional O₃) for 45 days. Both the control and the O₃chambers included non-fertilized and fertilized plants. After 18 days of O₃fumigation, seedlings were placed in a clean chamber for 27 days. Seedlings under ozone fumigation showed a significant decrease in pigment contents and photosynthetic activity, and a significant increase in lipid peroxidation. Fertilization enhanced physiological damage such as the inhibition of photosynthetic activity and the increase of lipid peroxidation under ozone fumigation. During the recovery phase, the physiological damage level of seedlings increased with ozone fumigation. In addition, physiological damage was observed in the fertilized seedlings. Superoxide dismutase (SOD) and glutathione reductase (GR) activities of O₃-treated seedlings increased up to 33.8% and 16.3% in the fertilized plants. The increase of SOD activity was higher in the fertilized plants than in the non-fertilized plants. Negative effects of ozone treatment were observed in the biomass of the leaves and the total dry weight of the fertilized sycamore seedlings. The O₃-treated seedlings decreased in stem, root and total dry weight, and the loss of biomass was statistically significant in the fertilized plants. In conclusion, physiological disturbance under normal nutrient conditions has an effect on growth response. In contrast, in conditions of energy shortage, although stress represents a physio-logical inhibition, it does not seem to affect the growth response.
- keywords
- Biomass, Lipid peroxidation, Pigment content, Photosynthetic activity, Recovery phase, Biomass, Lipid peroxidation, Pigment content, Photosynthetic activity, Recovery phase
Reference
Anderson PD, Palmer B, Houpis JLJ, Smith MK, Pushnik JC. 2003. Chloroplastic responses of ponderosa pine (Pinus ponderosa) seedlings to ozone exposure. Environt Int 29: 407-413.
Beauchamp C, Fridovichi I. 1971. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal Biochem 44: 276-297.
Bíelenberg DG, Lynch JP, Pell EJ. 2001. A decline in nitrogen availability affects plan responses to ozone. New Phytol 151: 413-425.
Boyer JS. 1971. Recovery of photosynthesis in sunflower after a period of low leaf water potential. Plant Physiol 47: 816-820.
Brewer RF, Guillemet FB, Creveling RK. 1961. Influence of N-P-K fertilization on incidence and severity of oxidant injury to mangels and spinach. Soil Sci 92: 298-301.
Calatayud A, Barreno E. 2004. Response to ozone in two lettuce varieties on chlorophyll a fluorescence, photosynthetic pigments and lipid peroxidation. Plant Physiol Biochem 42: 549-555.
Calatayud A, Pomares F, Barreno E. 2006. Interactions between nitrogen fertilization and ozone in watermelon cultivar Reina de Corazones in open-top chambers. Effects on chlorophyll a fluorescence, lipid peroxidation, and yield. Photosynthetica 44: 93-101.
Carlberg I, Mannervik B. 1985. Glutathione reductase. Method Enzymo. 113: 485-490.
Darrall NM (1989) The effect of air pollutants on physiological processes in plants. Plant Cell Environ 12: 1-30.
Dizengremel P, Petrini M. 1994. Effects of air pollutants on the pathways of carbohydrate breakdown In Plant Response to the Gaseous Environment. Molecular, Metabolic and Physiological Aspects. (Alscher RG, Wellburn AR, eds). Chapman and Hall, London. pp 255-278.
Evans JR. 1987. The dependence of quantum yield on wavelength and growth irradiance. Aust J Plant Physiol 14: 69-79.
Farage PK. 1996. The effect of ozone fumigation over one season on photosynthesis processes of Quercus robur seedling. New Phytol 134: 279-285.
Farquhar GD, Caemmerer von S, Berry JA. 1980. A biochemical model of photosynthetic CO2 assimilation in leaves of C3 species. Planta 149: 78-90.
Fossati P, Prencipe L, Berti G. 1980. Use of 3,5-dichloro-2-hydroxy benzenesulfonic acid /4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine. Clin Chem Methodol 26: 227-231.
Han SH, Kim DH, Lee KY, Ku JJ, Kim PG. 2007. Physiological damages and biochemical alleviation to ozone toxicity in five species of genus Acer. J Korean For Soc 96: 551-560.
Heath RL. 1996. Possible mechanisms for the inhibition of photosynthesis by ozone. Photosynth Res 39: 439-451.
Heath RL. 1994. The modification of photosynthetic capacity induced by ozone exposure. In Photosynthesis and the Environment (Baker NR, eds). Kluwer Academic Publishers, Dordrecht. pp 469-476.
Heath RL, Parker L. 1968. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125: 189-198.
Heck WW, Taylor OC, Tingey DT. 1988. Assessment of crop loss from air pollutants. Elsevier Applied Science. London. UK.
Heckathorn SA, DeLucia EH, Zielinski RE. 1997. The contribution of drought-related decreases in foliar nitrogen concentration to decreases in photosynthetic capacity during and after drought in prairie grasses. Physiol Plant 101: 173-182.
Kainulainen P, Utriainen J, Holopainen JK, Oksanen J, Holopainen T. 2000. Influence of elevated ozone and limited nitrogen availability on conifer seedlings in an open-air fumigation system: effects on growth, nutrient content, mycorrhiza, needle ultrastructure, starch and secondary compounds. Global Change Biol 6: 345-355.
Kangasjärvi J, Talvinen J, Utrianen M, Karjalainen R. 1994. Plant defense systems induced by ozone. Plant Cell Environ 17: 783-794.
Karlsson PE, Medin EL, Wickström H, Selldén G, Wallin G, Ottoson S, Skärby L. 1996. Interactive effects of ozone and drought stress on the growth of Norway spruce (Picea abies (L.) Karst.). In Critical Levels for Ozone - A Review of the Latest Rt Rtts from Experiments with Crops, Wild Plants and Forest Tree Species in the Nordic Countries (Skärby L, Pleijel H, eds). Swedish Environmental Research Institute, Göteborg, Sweden. pp 66-71.
Kim PG, Lee EJ. 2001. Ecophysiology of photosynthesis 1: Effects of light intensity and intercellular CO2 pressure on photosynthesis. Korean J Agr Forest Meteorol 3: 126-133.
Lee JC, Oh CY, Han SH, Kim PG. 2006. Photosynthetic inhibition in leaves of Ailanthus altissima under O3 fumigation. J Ecol Field Biol 29: 41-47.
Leone IA, Brennan E, Dainer RH. 1966. Effect of nitrogen nutrition on the response of tobacco to ozone in the atmosphere. J Air Pollut Contr Assoc 16: 191-196.
Lichtenthaler HK. 1987. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Method Enzymol 148: 350-382.
Lütz C, Anegg S, Gerant D, Alaoui-Sossé B, Gérard J, Dizengremel P. 2000. Beech trees exposed to high CO2 and to simulated ozone levels: effects on photosynthesis, chloroplast components and leaf enzyme activity. Physiol Plant 109: 252-259.
Manninen AM, Utriainen J, Holopainen T, Kainulainen T. 2002. Terpenoids in wood of Scots pine and Norway spruce seedlings exposed to ozone at different nitrogen availability. Can J For Res 32: 2140-2145.
Matyssek R, Günthardt-Goerg MS, Keller T, Scheidegger C. 1991. Impairment of the gas exchange and structure in birch leaves (Betula pendula) caused by low ozone concentrations. Trees 5: 5- 13.
Maurer S, Matyssek R, Günthardt-Goerg MS, Landolt W, Einig W. 1997. Nutrition and the ozone sensitivity of birch (Betula pendula) 1. Responses at the leaf level. Trees 12: 1-10.
Noctor G, Foyer CH. 1998. Ascorbate and glutathione: keeping active oxygen under control. Annu Rev Plant Physiol Plant Mol Biol 49: 249-279.
Ormrod DP, Adedipe NO, Hofstra G. 1973. Ozone effects on growfec of radish pof radis influenced by nitrogen and phosphorus nutrition and by temperature. Plant Soil 39: 437-439.
Ottoson S, Wallin G, Skärby L, Karlsson PE, Medin EL, Ränfors M, Pleijel H, Selldén G. 2003. Four years of ozone exposure at high or low phosphorus reduced biomass in Norway spruce. Trees 17: 299-307.
Pääkkönen E, Holopainen T. 1995. Influence of nitrogen supply on the response of clones of birch (Betula pendula Roth.) to ozone. New Phytol 129: 595-603.
Pell EJ, Sinn JP, Vinten-Johansen C. 1995. Nitrogen supply as a limiting factor determining the sensitivity of Populus tremuloides Michx. to ozone stress. New Phytol 130: 437-446.
Pell EJ, Winner WE, Vinten-Johansen C, Mooney HA. 1990. Response of radish to multiple stresses. I. Physiological and growth responses to changes in ozone and nitrogen. New Phytol 115: 439- 446.
Ranieri A, D'Urso G, Nali C, Lorenzini G, Soldatini GF. 1996. Ozone stimulates apoplastic antioxidant systems in pumpkin leaves. Physiol Plant 97: 381-387.
Renaud JP, Laitat E, Maulfette Y, Allard G. 1998. Photoassimilate allocation and photosynthetic and biochemical characteristics of two alfalfa (Medicago sativa) cultivars of different ozone sensitivities. Can J Bot 76: 281-289.
Ro HM, Kim PG, Lee IB. 2001. Photosynthetic characteristics and growth responses of dwarf apple (Malus domestica Borkh. cv. Fuji) saplings after 3 years of exposure to elevated atmospheric carbon dioxide concentration and temperature. Trees 15: 195-203.
Schubert R, Fischer R, Hain R, Schreier PH, Bahnweg G, Ernst D, Sandermann H. 1997. An ozone-responsive region of the grapevine resveratrol synthase promoter differs from the basal pathogen- responsive sequence. Plant Mol Biol 34: 417-426.
Sharp RE, Mattews MA, Boyer JS. 1984. Kok effect and the quantum yield of photosynthesis: Light partially inhibits dark respiration. Plant Physiol 75: 95-101.
Skärby L, Troeng E, Boström CA. 1987. Ozone uptake and effects on transpiration, net photosynthesis, and dark respiration in Scots pine. For Sci 33: 801-808.
Thomas VFD, Hiltbrunner E, Braun S, Flückiger W. 2002. Changes in root starch contents of mature beech (Fagus sylvatica L.) along an ozone and nitrogen gradient in Switzerland. Phyton 42: 223-228.
Thomas VFD, Braun S, Flückiger W. 2005. Effects of simultaneous ozone exposure and nitrogen loads on carbohydrate concentrations, biomass, and growth of young spruce trees (Picea abies). Environ Pollut 137: 507-516.
Thomas VFD, Braun S, Flückiger W. 2006. Effects of simultaneous ozone exposure and nitrogen loads on carbohydrate concentrations, biomass, growth, and nutrient concentrations of young beech trees (Fagus sylvatica). Environ Pollut 143: 341-354.
Tuomainen J, Pellinen R, Roy S, Kiiskinen M, Eloranta T, Karjalainen R, Kangagsjärvi J. 1996. Ozone affects birch (Betula pendula Roth) phenylpropanoid, polyamine and active oxygen detoxifying pathways at biochemical and gene expression level. J Plant Physiol 148: 179-188.
Utriainen J, Holopainen T. 2001a. Influence of nitrogen and phosphorus availability and ozone stress on Norway spruce seedlings. Tree Physiol 21: 447-456.
Utriainen J, Holopainen T. 2001b. Nitrogen availability modifies the ozone responses of Scots pine seedlings exposed in an open-field system. Tree Physiol 21: 1205-1213.
Utriainen J, Janhunen S, Helmisaari HS, Holopainen T. 2001. Biomass allocation, needle structural characteristics and nutrient composition in Scots pine seedlings exposed to elevated CO2 and O3 concentrations. Trees 14: 475-484.
Wallin G, Skärby L, Selldén G. 1990. Long term exposure of Norway spruce, Picea abies, to ozone in open top chambers, I. Effects on the capacity of net photosynthesis, dark respiration and leaf conductance of shoots of different ages. New Phytol 115: 335-344.
Wallin G, Karlsson PE, Selldén G, Ottoson S, Medin EL, Pleijel H, Skärby L. 2002. Impact of four years exposure to different levels of ozone, phosphorus and drought on chlorophyll, mineral nutrients, and stem volume of Norway spruce, Picea abies. Physiol Plant 114: 192-206.
Widodo W, Vu JCV, Boote KJ, Baker JT, Allen Jr, LH. 2003. Elevated growth CO2 delays drought stress and accelerates recovery of rice leaf photosynthesis. Environ Exp Bot 49: 259-272.
Willekens H, van Camp W, van Montagu M, Inzé D, Langebartels C, Sandermann H. 1994. Ozone, sulfur dioxide and ultraviolet B have similar effects on mRNA accumulation of antioxidant genes in Nicotiana plumbaginifolia (L.). Plant Physiol 106: 1007-1014.
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations