- ENGLISH
- P-ISSN2287-8327
- E-ISSN2288-1220
- SCOPUS, KCI
Species delimitation of the genus Champia (Rhodymeniales,Rhodophyta) from Korea using DNA barcoding
김명숙 (제주대학교)
조가연 (국립생물자원관)
Abstract
DNA barcoding is becoming a widely applied tool to accurately discriminate red algae. We tested the effectiveness of DNA barcoding for identification and discovery of Champia species in Korea and clarified the phylogenetic relationships using the plastid rbcL gene. As results, we described four species of Champia such as C. inkyua sp. nov., C. recta Noda, C. bifida Okamura, and C. expansa Yendo. A new species, C. inkyua, is characterized by entangled thallus, terete and irregular branches, hooked apices, and longitudinal filaments running throughout the frond periphery only. Longitudinal filaments were composed of a complete cell with two half cells between diaphragms in the cavity. C. recta and C. bifida were reinstated with previously used names of C. parvula and C. compressa, respectively. C. recta is the first recorded species from Korea and is characterized by an erect thallus, terete and irregular branches, and straight apices. C. bifida is characterized by compressed thallus, pinnate or alternate branches, and bifid apices. C. expansa is characterized by flabellate thallus and dichotomous branches. Molecular analyses of COI and rbcL genes revealed sufficient sequence divergence to warrant species recognition in the genus Champia.
- keywords
- Champia inkyua sp. nov., DNA barcoding, Rhodymeniales, taxonomy
참고문헌
Agardh CA. 1824. Systema Algarum. Literis Berlingianis, Lund.
Agardh JG. 1876. Species Genera et Ordines Algarum seu Descriptiones Succinctae Specierum, Generum et Ordinum quibus Algarum regnum Constituitur. 3, (i), Epicri sis Systematis Floridearum. TO Weigel, Leipzig.
Ballantine DL, Lozada-Troche C. 2008. Champia harveyana sp. nov. (Champiaceae, Rhodophyta) from Puerto Rico, Caribbean Sea. Botanica Marina 51: 388-398.
De Clerck O, Bolton JJ, Anderson RJ, Coppejans E. 2005. Guide to the Seaweeds of KwaZulu-Natal. National Botanic Garden of Belgium, Meise.
Desvaux NA. 1809. Observations sur le genre Fluggea, Rich. (Slateria, Desv.). J Bot (Desvaux) 1: 243-246.
Freshwater DW, Tudor K, O`Shaughnessy K, Wysor B. 2010. DNA barcoding in the red algal order Gelidiales: comparison of COI with rbcL and verification of the “barcoding gap”. Cryptogamie Alologie. 31: 435-449.
Guiry MD, Guiry GM. 2013. AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. http://www.algaebase.org. Accessed 24 June 2013.
Hall TA. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95-98.
Harvey WH. 1838. The Genera of South African Plants: arranged according to the natural system. AS Robertson, Cape Town.
Harvey WH. 1853. Nereis boreali-americana; or, contributions towards a history of the marine algae of the Atlantic and Pacific coasts of North America. Part II. Rhodospermeae. Smithsonian Institution, Washington.
Irvine LM, Guiry MD. 1983. Rhodymeniales. In: Seaweeds of the British Isles. Volume 1. Rhodophyta. Part 2A. Cryptonemiales (sensu stricto), Palmariales, Rhodymeniales. (Irvine LM, ed). British Museum (Natural History), London, pp 73-98.
Kim MS, Kang JC. 2011. A new Korean red algal species, Haraldiophyllum udoensis sp. nov. (Delesseriaceae, Rhodophyta). Algae 26: 211-219.
Kim MS, Kim SY, Yang MY, Kim B, Diaz-Tapia P. 2012. Morphology and molecular study of Pterosiphonia arenosa sp. nov. (Rhodomelaceae, Rhodophyta) from Jeju Island, Korea. Algae 27: 259-268.
Kim MS, Yang MY, Cho GY. 2010. Applying DNA barcoding to Korean Gracilariaceae (Rhodophyta). Cryptogam Algol 31: 387-401.
Le Gall L, Saunders GW. 2010. DNA barcoding is a powerful tool to uncover algal diversity: a case study of the Phyllophoraceae (Gigartinales, Rhodophyta) in the Canadian flora1. J Phycol 46: 374-389.
Lee IK. 1978. Studies on Rhodymeniales from Hokkaido. J Fac Sci Hokkaido Univ Ser 5 Bot 11: 1-194.
Lee YP, Kang SY. 2001. A Catalogue of the Seaweeds in Korea. Jeju National University Press, Jeju.
Lozada-Troche C, Ballantine DL. 2010. Champia puertoric- ensis sp. nov. (Rhodophyta: Champiaceae) from Puerto Rico, Caribbean Sea. Botanica Marina 53: 131-141.
Masuda M, Kogame K, Kawaguchi S, Phang SM. 2001. Taxonomic Notes on Marine Algae from Malaysia. V. Five Species of Rhodymeniales (Rhodophyceae). Botanica Marina 44: 81-88.
Millar AJK. 1990. Marine red algae of the Coffs Harbour region, northern New South Wales. Aust Syst Bot 3: 293- 593.
Millar AJK. 1998. Champia womersleyi (Champiaceae, Rhodophyta), a Flattened and Dichotomous New Species from South-Western Pacific. Botanica Marina 41: 15-21.
Noda M. 1973. Some marine algae collected on the coast of Kashiwazaki Province facing the Japan Sea (2). Sci Rep Niigata Univ Ser D (Biol) 10: 1-10.
Okamura K. 1901. Illustrations of the Marine Algae of Japan. Vol. 1. Part 5. Keigyosha, Tokyo, pp 67-98.
Okamura K. 1923. Icones of Japanese Algae. Vol. 4. Kazamashobo, Tokyo, pp 111-112.
Okamura K. 1931. Icones of Japanese Algae. Vol. 6. Part 6. Kazamashobo, Tokyo, pp 55-56 (Pl.279).
Park MR, Lee IK. 1998. Morphology and Phenology of Champia expansa Yendo and C. compressa Harvey (Rhodymeniales, Rhodophyta) from Korea. Algae 13: 85-99.
Reedman DJ, Womersley HBS. 1976. Southern Australian species of Champia and Chylocladia (Rhodymeniales: Rhodophyta). Trans R Soc S Aust 100: 75-104.
Ronquist F, Huelsenbeck JP. 2003. MrBayes3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572-1574.
Saunders GW. 2005. Applying DNA barcoding to red macroalgae: a preliminary appraisal holds promise for future applications. Philos Trans R Soc Lond B Biol Sci 360: 1879–1888.
Saunders GW, McDonald B. 2010. DNA barcoding reveals multiple overlooked Australian species of the red algal order Rhodymeniales (Florideophyceae), with resurrection of Halopeltis J. Agardh and description of Pseudohalopeltis gen. nov. Botany 88: 639-667.
Sherwood AR, Kurihara A, Conklin KY, Sauvage T, Presting GG. 2010. The Hawaiian Rhodophyta Biodiversity Survey (2006-2010): a summary of principal findings. BMC Plant Biol 10: 258.
Stamatakis A. 2006. RAxML-VI-HPC: Maximum Likelihoodbased Phylogenetic Analyses with Thousands of Taxa and Mixed Models. Bioinformatics 22: 2688-2690.
Suzuki M, Hashimoto T, Nakayama T, Yoshizaki M. 2010. Morphology and molecular relationships of Leptofauchea rhodymenioides (Rhodymeniales, Rhodophyta), a new record for Japan. Phycological Res 58: 116-131.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol Biol Evol 28: 2731-2739.
Womersley HBS. 1996. The Marine Benthic Flora of Southern Australia Part IIIB. Gracilariales, Rhodymeniales, Corallinales and Bonnemaisoniales. Australian Biological Resources Study, Canberra.
Yang MY, Han HG, Kim MS. 2013. Molecular identification of Grateloupia elliptica and G. lanceolata (Rhodophyta) inferred from plastid rbcL and mitochondrial COI genes sequence data. Gens Genom 35: 239-246.
Yendo K. 1903. Three new marine algae from Japan. Bot Mag Tokyo 17: 101-104.
Yoshida T. 1998. Marine algae of Japan. Uchida Rokakuho Publishing Co. Ltd., Tokyo.
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