Abstract

Objectives: This study was performed to investigate the effects of Smilacis Glabrae Rhizoma on antioxidation and antimicrobial actvity. Methods: In this work, as investgating the peroxide radicals on hydrogen donating activity and linoleic acid, and the MDA contents on the hepatic lipid of the rat, for the methanol extractions and the subfractions of Smilacis Glabrae Rhizoma, we obtained the followings. Results: 1. Hydrogen donating activity were very great for the radical scavenging effects, depending on the additional concentration at the fraction level of Chloroform and Ethyl acetate. 2. The peroxide radicals on linoleic acid were lower depending on the additional concentration, at the fraction level of Ethyl acetate, than the contrasts. So we obtained from the work that both had significant anti-oxidation effects. 3. MDA contents of the hepatic lipid had also the inhibition effects on the lipid radicals, in propotion to the concentration at n-Hexane, Chloroform, and Ethyl acetate fraction level. 4. After extracting Smilacis Glabrae Rhizoma with 80% methanol, we experimented with the extracts the antibiosis each concentration, for 5 bacilli, Bacilus subtilis, staphylococcus aureus, Escherichia coli, Salmonela typhimurium, Alcaligenes faecalis. While the effects showed differentiations by concentration, they had usually the significant inhibition effect for the multiplication at 37.5～75㎍/㎖. To identify the effective constituents, we identified the antibiosis of the fractions assaying cycliclly Hexane, Chloroform, Ethyl acetate, and Butanol. Then the result showed that antimicrobial activation of both Gram-positive and Gram-negative bacillus except for E. coli was measured highest at the fraction level of BuOH and Water. Conclusions: This result suggest that the extractions of Smilacis Glabrae Rhizoma, at Ethyl acetate fraction, had significant anti-oxidation effects and at BuOH and Water fraction had relatively strong antimicrobial activity against Bacilus subtilis, staphylococcus aureus, Salmonela typhimurium, Alcaligenes faecalis.