- P-ISSN 1010-0695
- E-ISSN 2288-3339
- Apply for Authority
- P-ISSN1010-0695
- E-ISSN2288-3339
Article Detail
Home > Article Detail
Effects of Bojeongjeongcheon-Tang on Cytokines and Immunoglobulin E in B Cells
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2004, v.25 no.2, pp.51-66
&
(2004). Effects of Bojeongjeongcheon-Tang on Cytokines and Immunoglobulin E in B Cells. Journal of Korean Medicine, 25(2), 51-66.
- Downloaded
- Viewed
Abstract
Objectives : To evaluate experimentally the clinical effect of Bojeongjeongcheon- tang, we observed the cytokines (IL-1β, IL-4, IL-5, IL-6, IL-10, TNF-α, TGF-β, IFN-γ) and how they have effect on IgE in B cells of a rat. Methods : First of all, we picked the spleen of healthy Balb/c mice and separated B cells from it. And the B cells were cultured with anti-CD40 mAb(500 ng/ml), rmIL-4(500 U/ml), Bojeongjeongcheon-tang (100 ㎍/㎖, 10 ㎍/㎖, 1 ㎍/㎖). We used rmIL-10 (50 ng/ml) as a control group. Furthermore, we analyzed the expression of IgE, CD23, CD69 and the coherence of HRF in B cells using flow cytometer. We also analyzed the cytokine gene expression in B cells by reverse transcriptase-PCR. And we also measured B cells proliferation using the Liquid Scintillation Counter. Results : In this study, Bojeongjeongcheon-tang treated group showed a tendency to decrease depending on the density compared with the control group in the expression of IgE+, CD23+, CD69, HRF. All of the Bojeongjeongcheon-tang treated group showed inhibitory effects with IL-1β, IL-4, IL-5 and proliferous effects with IL-6, IL-10, IFN-γ on Cytokines Transcript Expression depending on the density. Meanwhile, TNF-α increased in all density. In IgE production, there was inhibitory effect on Bojeongjeongcheon-tang (both 100㎍/㎖ and 10㎍/㎖) with significancy (p＜0.01, p＜0.05). Also in B cell proliferation, the result revealed inhibitory effect on Bojeongjeongcheon-tang(both 100㎍/㎖ and 10㎍/㎖) with significancy (p＜0.001, p＜0.01). Conclusions : This study shows that Bojeongjeongcheon-tang has an inhibitory effect on the production and activity of B cells. Also it inhibited CD23, IL-4 activity and IgE production and activation. It is obvious that Bojeongjeongcheon-tang cure asthma by inhibiting the production of histamine and HRF, IL-5 and proliferating IL-10. Also Bojeongjeongcheon-tang has some preventive effects on bronchial change by inhibiting TGF-β, which stimulates the bronchial transformation.
- keywords
- asthma, Bojeongjeongcheon-tang, cytokine, IgE, CD23, CD69, asthma, Bojeongjeongcheon-tang, cytokine, IgE, CD23, CD69
Reference
1
Donna E, (2003) Puddicombe, and Stephen T. Holgate. Airway remodeling in asthma,
2
Barnes PJ, (1998) Inhibitory cytokines in asthma,
3
Busse WW, (1998) Inflammation in asthma-the cornerstone of the disease and target of therapy,
4
Asky JA, (1997) Interleukins involved in the pathogenesis of chronic airway inflammation,
5
Palma Carlos AG,, (1997) Cytokines and asthma.,
6
Peter J, (2001) Cytokine modulators for allegic diseases. ,
7
M. Branco Ferreira , (1998) Cytokines and asthma,
8
강재성 외, (2002) 세포분자면역학, 범문사
9
김우경, (2002) 일차 진료인을 위한 천식 및 알레르기에 대한 이해,
10
전국한의과대학 폐계내과학교실 편저, (2002) 東醫肺系內科學, 한문화사
11
정광진, (2002) 瀉白散과 瀉白散加沙蔘이 人間 氣管支上皮細胞의 IL-6 IL-8 및 GM-CSF 發顯에 미치는 影響,
12
金大謙, (2002) 桑白皮가 제1형 알레르기 천식 모델 흰쥐의 BALF內 免疫細胞 및 血淸 IgE에 미치는 影響, 경희대학교 한의과대학
13
李秉熙, (2002) 蒼耳子가 제1형 알레르기 천식 모델 흰쥐의 BALF內 免疫細胞 및 血淸 IgE에 미치는 影響, 경희대학교 한의과대학
14
염종훈 ; 정희재 ; 정승기 ; 이형구, (2003) 定喘湯과 定喘湯加減方이 알레르기 천식모델 흰쥐의 BALF內 면역세포 및 혈청 IgE에 미치는 영향,
15
정진용, (2003;24) 小靑龍湯과 小靑龍湯加沙蔘이 BEAS-2B 人間 氣管支上皮細胞의 IL-6 IL-8 및 GM-CSF mRNA level에 미치는 影響,
16
정승기, (2002) 小靑龍湯이 기관지천식 환자의 혈청 IL-4 IFN- 변화에 미치는 영향,
17
정승기 ; 황우석 ; 주창엽 ; 이재성 ; 조일현 ; 정희재, (2002) 淸上補下湯의 기관지천식환자에 대한 임상적 효과,
18
황우석, (2002) 小靑龍湯치료 기관지천식환자의 혈액내 호산구수와 혈청IgE 및 T림프구아형의 변화,
19
최인선, (2000) 대기오염과 천식 천식 및 알레르기,
20
R. J. Davies,, (1998) Why is allergy increasing?- environmental factor,
21
(1986;7) 哮喘의 原因 및 治法에 關한 硏究,
22
황우석 ; 이형구 ; 정승기 ; 최준용 ; 이재성 ; 주창엽 ; 정희재, (2003) 淸上補下湯의 기관지천식환자에 대한 스테로이드 절약효과,
23
Lanny J. Rosenwasser,, (2003) Allergic asthma and an anti-CD23 mA(IDEC-152). ,
24
David M. Dorfman,, (2002) CD69 expression correlates with expression of other markers of Th1 Tcell differentiation in peripheral T cell lymphomas,
25
Sammy S. Nag,, (2003) The effects of IL-5 on airway physiology and inflammation in rats,
26
Singh AD, (1997) Anti-IL-5 strategies as a potential treatment for asthma,
27
이경훈, (2000) 급성 천식환자에서 혈중 IL-6 및 Soluble IL-6 수용체의 변화 결핵 및 호흡기질환,
28
S. Tavakoli, , (2001) Prostgrlandin E2-induced IL-6 release by a human airway epithelial cell line,
29
John M,, (1998) Inhaled corticosteroids increase IL-10 but reduce MIP-1 and IFN- release from alveolar macrophages in asthma,
30
Zuany-Amorim C,, (1995) IL-10 inhibits antigen-induced cellular recruitment into the airways of sensitized mice,
31
Wen FQ,, (2002) IL-4 and IL-13 enhanced transforming growth factor beta2 production in cultured human bronchial epithelial cells is attenuated by IFN-,
32
Mckay S,, (1998) Angiotensin Ⅱ induces hypertropy of human airway smooth muscle cell expression of transcription factors and TGF-,
33
Noma T,, (1995) Patten of cytokine production by T cells from adolescents with asthma in remission after stimulation with Deamtophagoides farinae antigen,
34
Magnan AO,, (2000) Assessment of the Th1/Th2 paradigm in whole blood in atropy and asthma insreased IFN- producing CD8+ T cell in asthma,
35
Kosuke Oikawa,, (2002) Dioxin Stimulates Synthesis and Secretion of IgE-Dependent Histamin-Releasing Factor,
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations
Other articles from this issue
- Studies on the antioxidation and antimicrobial effect of Smilacis Glabrae Rhizoma
- The Anti-tumor Effect of Bojungikkeehapdaechilkitang with Doxorubicin in Colon-26
- Effects of CPs(combined preparation of Radix Salvia Mltiorrhiza, Radix Notoginseng, and Borneolum) on Hyperlipidemia
- The Apoptosis Inducing Effect of Radix Aconiti Extract in HepG2 Human Hepatoma Cells
- The Effects of Astragali Radix on bleomycin-induced lung fibrosis
- Effects of Bojeongjeongcheon-Tang on Cytokines and Immunoglobulin E in B Cells
- Protective Effects of Palmul-tang on hypoxia-induced apoptosis in H9c2 cardiomyoblast cells
- A Correlation Research of Appetite and Dining Habit According to Sasang Constitution
- Effects of Taeyeumjowee-tang on Loss in Body weight, Plasma Lipids and UCP I Revelation of Fated White Rats
- The Anti-tumor Effect of Soonkiwhajungtang with Doxorubicin in MKN-45 Conclusion
- The Clinical Effects of Gamichuongsangboha-tang (Jiaweiqingshangbuxia-tang)extract in Asthmatic Patients Based on Severity
- A paper on the present condition of Cheong-Gang(晴崗) medical chart -Means of acquisition, filing condition and research value
- Effects of Sohaphyang-won on the Gene Expression in a Hypoxic Model of Cultured Rat Cortical Cells
- Effect on Alzheimer's Disease by Sesim-tang in CT105-overexpressed SK-N-SH cell lines
- Sasang Typology from a Personality Perspective
- Influence of Herbal Medicine Coadministration on the INR in Stroke Patients Taking Warfarin
- Protective Effects of Jihwangeumja on Oxidative Stress-induced Injury of Human Umblical Vein Endothelial Cells
- Effects of Gwibitang on Glutamate-induced Death in Rat Neonatal Astrocytes
- Effect of JiaoTeng-Yuan(交藤圓) on Oxidation Stress Caused by D-galactose in Sprague-Dawley Rats
- Response to Bee Venom Acupuncture and Polymorphism of Interleukin 4 Receptor and Interleukin 10 in Rheumatoid Arthritis
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.