Article Detail

Home > Article Detail
  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

Effects of Sohaphyang-won on the Gene Expression in a Hypoxic Model of Cultured Rat Cortical Cells

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2004, v.25 no.2, pp.127-137






  • Downloaded
  • Viewed

Abstract

1. Objectives : The purpose of this investigation is to evaluate the effects of Sohaphyang-won(SH) on the alteration in gene expression in a hypoxia model using cultured rat cortical cells. 2. Methods : E18 rat cortical cells were grown in Neurobasal medium containing B27 supplement. On 12 DIV, SH was added (20㎍/㎖) to the culture media for 24 hrs. On 14 DIV, cells were given a hypoxic insult (2% O2/5% CO2, 37°C, 3 hrs), returned to normoxia and culture for another 24 hrs. Total RNA was prepared from SH-untreated (control) and -treated cultures and alteration in gene expression was analyses by microarray using rat 5K-TwinChips. 3. Results Effects on some of the genes whose functions are implicated in neural viability are as follows: 1) For most of the genes altered in expression, the Global M values were between -0.5 to +0.5. Among these, 1517 genes were increased in their expression by more than Global M +0.1, while 1480 genes were decreased by more than Global M -0.1. 2) The expression of apoptosis-related genes such as Bad (Global M = 0.35), tumor protein p53 (T53) (Global M = 0.28) were increased, while v-akt murine thymoma viral oncogene homolog 1(Akt1) was decreased. 3) The expression of hemoglobin alpha 1 (probably neuroglobin) was increased by about 3.2-fold (Global M = 1.7). 4) The expression of antioxidation-related catalase gene was increased (Global M = 0.26). 5) The expression of PKCzeta(prkcz), an upstream kinase of MAPK, was increased (Global M = 0.29). 6) The expression of retinoic acid receptor alpha (RARα), which may regulated transcription in hypoxic stress, was increased (Global M = 10.27). 4. Conclusions : In summary, the microarray data suggest that SH doesn't increase the expression of oxygen capture-, anti-oxidation- and 'Response to stress'-related genes but also decreases some anti-apoptosis genes which would help protect the hypoxiv cells from apoptosis.

keywords
Sohaphyang-won, Gene Expression, microarray, apoptosis, hypoxia, Sohaphyang-won, Gene Expression, microarray, apoptosis, hypoxia


Reference

1

Fernando MR, , (1992;207) Thioredoxin regenerates proteins inactivated by oxidative stress in endotherial cells,

2

Kontos H, (1985;57) Appearance of superoxide anion radical in cerebral extracellular space during increased prostaglandin synthesis in cat,

3

(1983;65) Rapid colormetric assay for cellular growth and survival Application to proliferation and cytotoxic assays,

4

Palmer C, (1990;27) Reduction of perinatal hypoxic-ischemic brain damage with allplurinol,

5

Kerr JFR, (1972;26) a basic biological phenomenon with wideranging implications in tissue kinetics,

6

고재영, (1998) 신경계질환에서의 아폽토시스,

7

Schenm M,, (1995) Quantitiative monitoring of gene expression patterns with a complementary DNA microarray,

8

Duggan DJ,, (Jan1999) Expression profiling using cDNA microarrays,

9

Whitney LW, (1999) Analysis of gene expression in multiple sclerosis lesions using cDNA microarrays,

10

차상현, (1995) 중풍치료의 문헌적 고찰과 침구치료에 대한 소견,

11

(1985) 太平惠民和劑局方, 人民衛生出版社

12

안영기, (1981) 四關穴 鍼刺와 麝香蘇合香元이 鎭痛 血淸成分變化 및 胃기능에 미치는 영향,

13

남상경, (1989) 牛黃淸心元과 蘇合香元의 효능에 관한 연구,

14

이영빈, (1997) 麝香蘇合元이 재관류장치하의 흰쥐 심장에 미치는 영향,

15

최은정, (1997) 蘇合香元이 실험적 뇌경색 흰쥐의 국소뇌혈류량 및 경색 면적에 미치는 영향,

16

이지훈 ; 윤경선 ; 정승현 ; 신길조 ; 이원철 ; 문일수, (2003) 蘇合香元이 저산소증 유발 배양 대뇌신경세포에 미치는 영향,

17

김재우, (2002) 배양한 흰쥐 대뇌세포의 저산소 및 저당/저산소증 모델에서 蘇合香元의 세포사 방지효과에 대한 연구,

18

Brewer G, Optimized survival of hippocampal neurons in B27-supplemented Neurobasal,

19

Brain Res, (1982;239) delayed neuronal death in the gerbil hippocampus following ischemia,

20

Tamura A, (1985;63) Selective vulnerability of the hippocampus to ischemia-reversible and irreversible types of ischemic cell damage Progress in Brain Res,

21

Wyllie AH, (1980;68) the significance of apoptosis,

22

원철환 ; 정승현 ; 신길조 ; 문일수 ; 이원철, (2002) 배양 대뇌신경세포의 저당-저산소증 모델에서 牛黃淸心丸에 의한 세포사 방지 연구,

23

Rashidi, H.H,, (2000) bioinformatics Basic Applications in Biological Science and Medicine, CRC Press

24

Schena M,, (1996) Parallel human genome analysis : microarray-based expression monitoring of 1000 genes : Proc.,

25

Schena, M,, (1995) Quantitative monitoring of gene expression patterns with a complementary dna microarray,

26

Kang JQ,, (2003sep) Critical role for Akt1 in the modulation of apoptotic phosphatidylserine exposure and microglial activation,

27

Sun Y, (2001) Neuroglobin is up-regulated by and protects neurons from hypoxic-ischemic injury,

28

Zhu Y, (2002October) Hemin induces neuroglobin expression in neural cells,

29

Martin R,, (2002dec) Effect of age and hypoxia/reoxygenation on mRNA expression of antioxidative enzymes in rat liver and kidneys,

30

Lievre V,, (2001) Intracellular generation of free radicals and modifications of detoxifying enzymes in cultured neurons from the developing rat forebrain in response to transient hypoxia,

31

Liu Yi, , (2000) Reaction intermediates and single turnover rate constants for the oxidation of heme by human heme oxigenase-1,

32

Stocker R, (1987;235) Bilirubin is an antioxidant of possible physiological importance,

33

Martinez-Cruz F,, (2002aug) Oxidative stress induced by phenylketonuria in the rat Prevention by melatonin vitamin E, and vitamin C,

34

Takeda H, Matozaki T, , (1999) PI 3-kinase gamma and protein kinase C-zeta mediate RAS-independent activation of MAP kinase by a Gi protein-coupled receptor,

35

Mizukami Y, (2000Jun) Nuclear mitogen-activated protein kinase activation by protein kinase czeta during reoxygenation after ischemic hypoxia,

36

Chauvet C,, (2002Jun) Retinoic acid receptor-related orphan receptor(ROR) alpha4 is the predominant isoform of the nucl hepatoma cellsear receptor RORalpha in the liver and is up-regulated by hypoxia in HepG2 human hepatoma cells,

  • Downloaded
  • Viewed
  • 0KCI Citations
  • 0WOS Citations

Other articles from this issue

Recommanded Articles

상단으로 이동

Journal of Korean Medicine