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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

Effect on Alzheimer's Disease by Sesim-tang in CT105-overexpressed SK-N-SH cell lines

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2004, v.25 no.2, pp.138-150



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Abstract

Objectives : Alzheimer's disease(AD) is a geriatric dementia that is widespread in old age. In the near future AD will be the biggest problem in public health service. Although a variety of oriental prescriptions in study Sesim-tang have been traditionally utilized for the treatment of AD, their pharmacological effects and action mechanisms have not yet fully elucidated. The present study investigated effects of Sesim-tang on apoptotic cell death induced by CT105 (carboxy terminal 105 amino acid peptide fragment of APP) overexpression in SK-N-SH neuroblastoma cell lines. Methods : We studied on the regenerative and inhibitory effects on Alzheimer's disease in CT105-induced SK-N-SH cell lines by Sesim-tang water extract. We were examined by Cell morphological pattern, DNA fragmentation, LDH activity assay, Zymography assay, immunohistochemistric analysis. Additionally, we investigated the association between the CT105 and neurite degeneration caused by CT105-induced apoptotic response in neurone cells. Results : Findings from our experiments have shown that Sesim-tang inhibits the synthesis or activities of CT105, which has neurotoxicities and apoptotic activities in cell line. In addition, pretreatment of Sesim-tang(>50㎍/㎖ for 12 hours) partially prevented CT105-induced cytotoxicity in SK-N-SH cell lines, and were inhibited by the pretreatment with its. SK-N-SH cell lines overexpressed with CT105 exhibited remarkable apoptotic cell damage. Based on morphological observations by phase-contrast microscope and LDH activity measurements in the culture media, the CT105-induced cell death was significantly inhibited by Sesim-tang water extract. Sesim-tang was found to reduce the expression of APP, caspase-3 induced by CT105 in SK-N-SH cell lines and in rat hippocampus. Conclusions : As the result of this study, in Sesim-tang group, the apoptosis in the nervous system is inhibited, the repair against the degerneration of SK-N-SH cell lines by CT105 expression is promoted. So Sesim-tang may be beneficial for the treatment of AD.

keywords
Alzheimer's disease(AD), CT105, Sesim-tang, Alzheimer's disease(AD), CT105, Sesim-tang


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