ISSN : 1225-3480
본 연구에서는 남극삿갓조개 (Nacella concinna) 의 장 (intestine) 의 중금속 농축에 관하여 알아보고자 카드뮴 노출실험을 실시한 후 중금속에 노출 시 동물체내에서 유도되는 단백질 중의 하나인 metallothionein을 면역조직화학적 방법으로 추적하고 투과전자현미경 (transmission electron microscope) 을 이용하여 세포의 미세구조의 변화를 알아보았다. 더불어 SEM-EDS 장비를 이용한 원소분석을 통해 중금속의 분포를 알아보아 중금속의 축적 및 해독기작에 대한 기초자료를 얻고자 수행되었다. Metallothionein의 분포를 살펴보기 위하여 면역조직화학적 실험이 수행된 바, 중금속이 농축되고 있는 삿갓조개의 장상피세포의 첨단부에 metallothionein이 많이 존재하고 있음을 알 수 있었으며, 미세구조 관찰결과 노출시간이 경과함에 따라 핵막의 팽창, whorl 구조의 출현, 핵 내 봉입체를 관찰할 수 있었다. SEM-EDS 관찰결과 카드뮴 노출결과, 황이 급격하게 줄어들었고, 칼슘과 아연이 상대적으로 증가하는 양상을 관찰 할 수 있었다. 이상에서와 같이 Cd 노출의 정도에 따라 비교적 빠른 세포반응을 보이는 남극삿갓조개의 특징은 자연 상태에서 Cd의 노출에 따른 효과적인 생물 지표종으로서 가치가 있는 것으로 사료된다.감사의 말씀본 연구과제는 2002학년도 순천향대학교 학술연구조성비 교내연구과제 및 한국해양연구원 남극기본연구활동지원 사업의 위탁과제 연구비로 수행되었습니다.
Immunohistochemical and ultrastuructural experi- ments were conducted to find out heavy metal accumulation in the intestine of an Antarctic gastropod Nacella concinna. According to the immuno- histochemical experiment the apical cytoplasm of the intestinal epithelium showed positive reactions to anti-MT (metallothionein), indicating the presence of MT, a metal-binding protein involved in metal detoxifying process. In the transmission electron microscopic observations, the epithelial cells of the intestine exposed to Cd for over three hours showed irregular nuclear membranes, secretory granules, and probable metal granules. According to the SEM-EDS experiments on the intestine, concentration of Pb in the apical epithelium was in inverse proportion to that in the intestinal lumen. After exposing to Cd for over three days, S was rapidly reduced. Ca and Zn were rapidly increased after exposure to Cd. These elements are supposed to be concerned with the MT-reaction in the intestine. Taken together, these data suggest that N. concinna could be used as a potential biomarker species.
(1994) Ecology and biology of the Antarctic soft-shelled clam Korean Journal of Malacology 10 [in Korean with English Abstract],
(2001) The effect of body size on metal accumulations in the bivalve Laternula elliptica,
(2002) A baseline study on metal concentrations in the Antarctic limpet Nacella concinna (Gastropoda: Patellidae) on King George Island: variations with sex and body parts,
(1996) Baseline heavy metal concentrations in the Antarctic clam, Laterrula elliptica in Maxwell bay, King George island, Antarctica,
(1994) growth and reproduction of the limpet Nacella in relation to potential food availability,
(1999) PROFILE: Environmental impact assessment under the national environmental policy act and the protocol on environmental protection to the Antarctic treaty,
(2002) Induction of specific isoforms of metallothionein in mussel tissues after exposure to cadmium or mercury,
(1998) Regulation of the synthesis of brain metallothioneins,
(1987184-197) Distribution of heavy metals in Antarctic marine ecosystem Proceedings of NIPR Symposium on Polar Biology,
(1992) Tissue and sub-cellular distribution of Fe Zn and 210Po in the abalone Haliotis rubra,
(2000) Immunohistochemical localization of metallothionein in endometrial lesions ,
(2000) Correlation of metallothionein expression with apoptosis in nasopharyngeal carcinoma,
(2000) Histochemical characterization of silver-induced metallothionein in rat kidney,
(2002) An immunohistochemical and ultrastructural study of the heavy metals accumulated in the kidney of an Antarctic clam, Laternula elliptica,
(1990191-199) Cellular cadmium distribution in the common winkle determined by X-ray microprobe analysis and histochemistry,
(2001) Effects of copper and cadmium exposure on functional responses of hemocytes in the clam, Tapes philippinarum,
(1997) Heavy metals in Antarctic organisms,
(2000) Histopathology of the digestive gland of an Antarctic limpet exposed to cadmium,
(2000) In vivo gene expression profile analysis of metallothionein in renal cell carcinoma,
(1992) Ultrastructural localization of metal binding sites in the kidney of the Antarctic scallop Adamussium colbecki,
(1993) Cadmium in the gastropod Littorina littorea Journal of Marine Biology Assessment,
(1998) Evidence that the reactions of cadmium in the presence of metallothionein can produce hydroxyl radicals,
(1994) Monitoring of Temporal trends in chemical contamination by the NOAA National Status and Trends Mussel Watch Project Biomonitoring of Costal Waters and Estuaries, CRC Press
(1992) Role of metallothionein in human health,
(2000) Immunocyto- chemical localization of metallothionein in human pancreatic islets,
(1999) Metallothionein as a tool in biomonitoring programes,
(2000) Immunocytochemical localization of metallothionein and its relation to doxorubicin toxicity in transgenic mouse heart,
(2000) Immunocytochemical localization of metallothionein and its relation to doxorubicin toxicity in transgenic mouse heart,
