ISSN : 1225-3480
Many freshwater snail taxa are difficult to identify using morphological traits due to phenotypic plasticity. However, using of molecular DNA marker in combination with morphological traits can provide a reliable means for discriminating among freshwater snail taxa including cryptic species. To discriminate among Korean freshwater snail taxa and resolve their systematic relationships, wesequenced a fragment of mtDNA cytochrome oxidase I (COI) gene from 82 specimens collected from ten different sites distributed along the Korean peninsula. We identified more than seven freshwater snail taxa including cryptic species in Korea. Whereas traditional shell morphology of freshwater snails offers only weak discriminatory power for recognizing ‘good’ taxa, DNA sequence data provided positive and reliable identification. In addition, a major Semisulcospira clade was clearly separated from the remaining lineages observed including cryptic species. However, a phylogenetic tree inferred from the COI gene data did not fully resolve systematic relationships among pleurocerid taxa in Korea. Establishing more robust shell characteristics for identifying taxa unambiguously and hence improving traditional key shell morphology characters for freshwater snail species is an urgent requirement and will require more rigorous examination of all nominal taxa. While molecular data generated here will be useful for species identification and for describing the systematic relationships among Korean freshwater snails, further analysis will be required.
Asahida, T., Kobayashi, T., Saitoh, K. and Nakayama, I. (1996) Tissue preservation andtotal DNA extraction from fish stored at ambient temperature using buffers containing high concentration of urea. Fisheries Science, 62: 727-730.
Burch, J.B., Chung, P.R. and Jung, Y. (1987) A guide to the fresh water snails of Korea. Walkerana, 2:195-232.
Burch, J.B. and Jung, Y. (1987) A new freshwater prosobranch snail (Mesogastropoda: Pleuroceridae) from Korea. Walkerana, 2: 287-293.
Chambers, S.M. (1978) An electrophoretically detected sibling species of Goniobasis floridensis. Malacologia, 17: 157-162.
Chang, Y.J., Chang, H.J., Min, B.H. and Bang, I.C. (2000) Reproductive cycle of the melania snail, Semisulcospira libertina libertina. Development and Reproduction, 4: 175-180.
Chevenet, F., Brun, C., Banuls, A.L., Jacq, B. and Christen, R. (2006) TreeDyn: towards dynamic graphics and annotations for analyses of trees. BMC Bioinformatics, 7: 439.
Choi, B.R. and Yoon, S.H. (1997) Mollusca. In: List of animals in Korea. The Korean Society of Systematic Zoology, Seoul, Korea.
Davis, G.M. (1969) A taxonomic study of some species of Semisulcospira in Japan (Mesogastropoda:Pleuroceridae). Malacologia, 7: 211-294.
Felsenstein, J. (1989) PHYLIP – Phylogeny Inference Package (Version 3.2). Cladistics, 5: 164-166.
Folmer, D., Black, M., Hoeh, W., Lutz, R. and Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome C oxidase subunit I from diverse metazoan invertebrate. Molecular Marine Biology and Biotechnology, 3: 294–299.
Guindon, S. and Gascuel, O. (2003) PhyML - A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology, 52: 696-704.
Holznagel, W.E. and Lydeard, C. (2000) A molecular phylogeny of North American Pleuroceridae (Gastropoda: Cerithioidea) based on mitochondrial 16S rDNA sequences. Journal of Molluscan Studies, 66: 233-257.
Huesenbeck, J.P. and Ronquist, F. (2001) MRBAYES:Bayesian inference of phylogenetic trees. Bioinformatics, 17: 754-755.
Jeong, Y., Park, G.M., Park, J., Kim, J.J., Min, D.Y. and Chung, P.R. (1999) Allozyme variability in three species of genus Semisulcospira (Prosobranchia:Pleuroceridae). The Korean Journal of Malacology, 15: 13-20.
Kim, S.S., Kim, D.C., Chung, P.L. and Chung, Y.J. (1987) A cytological study on two species of genus Semisulcospira (Gastropoda: Pleuroceridae) in Korea. The Korean Journal of Malacology, 3: 24-34.
Kim, J.J. (1995) Isozyme variation of the genus Semisulcospira (Pleuroceridae: Gastropoda) in Korea. The Korean Journal of Malacology, 11: 171-179.
Ko, J.H., Lee, J.S. and Kwon, O.K. (2001) Study on radulae of seven species of the Family Pleuroceridae in Korea. The Korean Journal of Malacology, 17:105-115.
Kuroda, T. (1929) On the species of Japanese Kawnina (Semisulcospira). Venus, 1: 179-193.
Kwon, O.K. and Habe, T. (1979) A list of non-marine molluscan fauna of Korea. Korean Journal of Limnology, 12: 24-31.
Kwon, O.K. (1990) Illustrated encyclopedia of fauna and flora of Korea Vol. 32. Mollusca (I). Ministry of Education. Seoul, Korea.
Kwon, O.K., Park, G.M. and Lee, J.S. (1993) Colored shells of Korea. Academy publishing company. Seoul, Korea.
Lee, J.S., Ko, J.H. and Kwon, O.K. (2001) Isozyme variation in two species of freshwater Pleurocerid snails in Korea: Koreanomelania nodifila and Koreoleptoxis globus ovalis. The Korean Journal of Malacology, 17: 117-123.
Lee, T., Hong, H.C., Kim, J.J. and Ó Foighil, D. (2006) Crossing the continental divide: the Columbia drainage species Juga hemphilli (Henderson 1935) is a cryptic member of the eastern North American genus Elimia (Cerithioidea: Pleuroceridae). Journal of Molluscan Studies, 72: 314-317.
Lee, T., Hong, H.C., Kim, J.J. and Ó Foighil, D. (2007) Phylogenetic and taxonomic incongruence involving nuclear and mitochondrial markers in Korean populations of the freshwater snail genus Semisulcospira (Cerithioidea: Pleuroceridae). Molecular Phylogenetics and Evolution, 43: 386-397.
Lydeard, C., Holznagel, W.E., Garner, J., Hartfield, P. and Pierson, M. (1997) A molecular phylogeny of Mobile River drainage pleurocerid snails (Caenogastropoda: Cerithioidea). Molecular Phylogenetics and Evolution, 7: 117-128.
Lydeard, C., Yoder, J.H., Holznagel, W.E., Thompson, F.G. and Hartfield, P. (1998) Phylogenetic utility of the 5'-half of mitochondrial 16S rDNA gene sequences for inferring relationships of Elimia (Cerithioidea: Pleuroceridae). Malacologia, 39:183-193.
Martens, E. von (1886) In Japan und Korea gesammelten land-und Süßwasser-Mollusken. Sitzungs-Bericht der Gesellschaft naturforschender Freunde zu Berlin, 5: 76-78.
Martens E. von (1905) Koreanische Süßwasser-Mollusken. Zoologische Jahrbücher Supplement, 8:23-70.
Milton, R.L. and Lydeard, C. (2003) Phylogeny, taxonomy, genetics and global heritage ranks of an imperiled, freshwater snails genus Lithasia (Pleuroceridae). Molecular Ecology, 12: 75-87.
Miyanaga, M. (1942) Kwwanina snails of the genus Semisulcospira from Korea. Chosen Hakubutsu Zasshi. Korean Journal of Biology Science, 9:114-130.
Posada, D. and Crandall, K. (1998) MODELTEST:testing the model of DNA substitution. Bioinformatics, 14: 817–818
Rozas, J., Sánchez-DelBarrio, J.C., Messeguer, X. and Rozas, R. (2003)DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics, 19: 2496-2497.
Wilke, T. and Falinowski, A. (2000) The genus Adriohydrobia: polytpic species or polymorphic populations? Journal of Zoological Systematics and Evolutionary Research, 39: 227-234.
