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ISSN : 1225-3480
본 연구는 우리나라 무안, 태안, 여수, 제주 지역 및 중국 영성, 대련 지역의 낙지 개체군의 종 및 집단분석을 위해 미토콘드리아 CO1유전자의 염기서열을 조사하였다. 유전자 분석은총 60 개체로부터 6개의 haplotype이 조사되었다. 제주 개체군인 경우 모든 개체 (N = 10) 가 다른 집단에서는 관찰되지않은 A haplotype으로 구성되어 있어 다른 지역과 뚜렷한 차이를 나타내었다. 이들 haplotype은 MEGA 4 분석에 의해두 개의 clade로 나뉘어지는데 하나는 무안, 태안, 여수, 중국대련집단이었고 다른 하나는 제주, 중국 영성집단으로 나뉘는구조를 하고 있었다. 집단 간 유연관계 분석에서도 같은 현상이 관찰되었다. Group A에 속하는 무안, 태안, 여수, 대련 집단의 낙지 개체군은 group B (제주, 영성) 에 속하는 집단 개체군 보다 유전적 거리가 아주 가까웠다. 본 연구에서 사용한CO1 universal primer는 종 판별 유전자 마커로서 낙지류에서도 유용하게 활용될 수 있었으나 partial CO1 유전자 내의 변이가 많지 않기 때문에 집단분석에는 한계가 있으나, 지역적 거리 및 장벽 그리고 서식지 차이 등에서 오는 제한적gene flow에 대한 논의는 가능할 것으로 판단된다.
The nucleotide sequences of the mitochondrial cytochrome oxidase subunit 1 (CO1) gene of octopus groups collected from Muan, Taean, Yesu, Jeju in Korea and Youngsung, Daeryen in China were analyzed for the identification of species and populations. Six haplotypes were identified from the analyzed 60 individuals. All of the individuals (N = 10) from Jeju showed the A haplotype which was not observed from other groups, and could be classified as a distinct group. The analyzed groups could form two separate clade in MEGA4 analysis. The individuals from Muan, Taean, Yesu in Korea and Daeryen in China form a clase and the others from Jeju in Korea and Youngsung in China formed the other clade. The analysis of relationship among the groups showed the same results. Individuals belong to the group A (Muan, Taean, Yesu and Daeryen) showed closer relationship than individuals belong to the group B (Jeju and Youngsung). Although the CO1 universal primers used in this study was useful as a marker for species identification among Octopus, analysis of population was limited because of few variations in the partial sequences of CO1 analyzed in this study. However, it was possible to show the limited gene flow among the groups which is resulted from the spatial separation and differences in their habitats
Chase M.W., Salamin N., Wikinson M., Dunwell J.M., Kesanakurthi R.P., Haidar N. & Savolainen V. (2005) Land plants and DNA barcodes: Short-term and Long-term goals. Philosophical Transactions of the Royal Society Biological Sciences, 360: 1889-1895.
Folmer O., Black M., Lutz R. & Vrijenhoek R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebreates. Molecular Marine Biology and Biotechnology, 3: 294-299.
Hebert P.D.N., Ratnasingham S. & Waard J.R. De (2003) Barcoding animal life: cytochrome c oxidase subunit I divergences among closely related species. Philosophical Transactions of the Royal Society Biological Sciences, 270: 96-99.
Hebert P.D.N., Penton E.H., Burns J.M., Janzen D.H. & Hallwachs W. (2004) Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences of the United States of America, 101: 14812-14817.
Hillis D.M., Mabel B.K. & Moritz C. (1996) Application of molecular systematics: The state of the field and a look to the future. Molecular Systematics. Sinauer. Sunderland. 515-543.
Kim J.I., Oh T.Y., Seo Y.I. & Cho E.S. (2009) Population genetic structure of Octopus minor Sasaki from Korea and China based on a partial sequencing of mitochondrial 16S rRNA. Korean Journal of Life Science, 19: 711-719
Roper C.F.E., Sweeney M.J. & Nauen C.E. (1984) Cephalopods of the World: An annotated and illustrated catalogue of species of interest to fisheries. FAO. Fisheries Synopsis, 125(3).
Tamura K., Dudley J., Nei M. & Kumar S. (2007) MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24: 1596-1599.
Ward R.D., Zemlak T.S., Innes B.H., Last R.R. & Hebert P.D.H. (2005) DNA barcoding Australia's fish species. Philosophical Transactions of the Royal Society Biological Sciences, 360: 1847-1857.
Zhu D., Jamieson B.G.M., Hugall A. & Moritz C. (1994) Sequence evolution a and phylogenetic signal in control region and cytochrome b sequences of rainbow fishes. Molecular Biology and Evolution, 11:672-683.