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ISSN : 1225-3480
제주배꼽달팽이 (A. chejuensis) 의 metallothionein 유전자는 288 bp의 염기서열로 구성되어 있으며, 96개의 아미노산으로 이루어져 있다. 연체동물의 MT 특이적 서열 공식인 C-x-C-x (3) -C-T-G-x (3) -C-x-C-x (3) -C-x-C-K 에 대입해 보았을 때 알려진 공식과 일치하는 것을 확인하였다. BLAST 결과를 토대로 23종의 MT 서열을 얻었으며, 선행된 연구에서 사용된 5종의 MT 서열을 인위적으로 추가하여 data set를 구성하였다. ClustalX를 통해 multiple alignment하고, Neighbor-Joining 방법을 통해 phylogenetic tree를 그려본 결과 제주배꼽달팽이 (A. chejuensis) 의 MT 서열은 병안목(Stylommatophora) 에 속하는 A. quelpartensis, N. samarangae, H. pomatia, H. aspersa, S. myomphala 와 같은 그룹으로 묶이는 것을 확인 할 수 있었다. 이러한 근거를 토대로 MT 유전자 서열에 대한 연구들이 앞으로 더 많은 연체동물을 대상으로 수행된다면, 추후 분자계통학적 및 계통발생학적 방법의 연구에 유용하게 사용될 수 있는 마커로서의 충분한 가능성을 보인다는 것을 확인시켜 주었다.
Metallothioneins (MT) are cysteine-rich small proteins known for their high affinity binding to metal ions. MT confers cytoprotection against oxidative stress and acts as an efficient modulator of immunity and infection. There have been ever-increasing reports on the function of MT in environmental monitoring programmes. Lately, MT has been studied as a biomarker towards the taxonomic clustering of various species, including the molluscans. The Korean endemic land snail, Aegista chejuensis is native to Jeju Island and South coastal regions of Korea. Functional genomics information for the species is limited, although the Illumina-based DNAseq and transcriptome information are registered with the Sequence Read Archive (SRA) of National Center for Biotechnology Information (NCBI). While annotating the ORF-sensitive unigenes from the transcriptome, a MT sequence comprising of 288 bp coding region and 96 amino acid residues translatable protein was deciphered. In silico analysis using ClustalX and the MEGA7 program grouped A. chejuensis under the Stylommatophora clade of eupulmonate mollusk families. The study was able to confirm the evolutionary position of the species and established the MT sequence as a candidate for taxonomic classification of the species.
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