ISSN : 1225-3480
본 연구는 왕우럭조개의 산란 및 초기 배의 발생에 대해 연구하였다. 산란유도를 위해 잔연산란, 물리적자극 (수온, 간출), 담수자극 및 화학적자극 (정자현탁액) 을 시도하였다. 인공수정시 다정수정을 피하고 적정한 정자량을 파악하고자 정자량을 달리하여 수정을 유도한 후, 수정율 및 부화율을 조사하여 가장 효과적인 정자량을 조사하였다. 또한 수온과 염분이 초기배의 발생에 미치는 영향을 조사하였다. 연구 결과 물리적자극 (수온, 간출) 이 35%의 산란유도 효과를 보여 다른 자극 방법에 비해 우수함을 보였다. 정자량에 따른 수정율 및 부화율의 조사에서는 <TEX>$50{\times}10^4inds./egg$</TEX> 정자량이 가장 효과가 좋았다. 수온 및 염분이 배 발생의 속도에 미치는 영향을 살펴본 결과 수온은 높을수록, 염분 농도는 자연조건에 가까울수록 배의 발생이 빨랐다.
This study interested on spawning and early embryo development in surf clam, Tresus keenae. For spawning induction, we performed natural spawning, physical stimulus (temperature, air dry), freshwater stimulus and chemical stimulus (sperm suspension). In addition, the sperm amounts were differently treated during artificial insemination and were investigated optimal amount of sperm through fertilization rate and hatching rate. The effects of water temperature and salinity on early embryo development were also investigated. Result that, the physical stimulation method was the most effective method with 35% efficiency in inducing spawning. The fertilization rate and hatching rate were best when 50 × 104 inds./egg sperm were used for artificial insemination. The effects of water temperature and salinity on reaction velocity of embryogenesis were investigated. The fast velocity of embryogenesis had the water temperature was higher and salinity concentration was close to natural condition.
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