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ISSN : 1225-3480
- 두타산입술대고둥아재비 (M. junensis) 의 Arginine kinase 유전자는 1,074 bp, 아미노산 358 개로 이루어져 있다. - BLAST를 통해 얻은 13 종의 ArK 참고서열들과 두타산입술대고둥아재비의 ArK 서열을 다중서열정렬한 결과 ArK 특이 잔기가 보존되어 있음을 알 수 있었다. - MEGA7 소프트웨어를 활용하여 multiple alignment 한 후, Maximum-Likelihood 방법으로 Molecular phylogenetic analysis 를 수행한 결과, N. samarangae, A. kurodai, B. glabrata, S. libertinam, C. ebraeus, C. grata 와 같은 복족강들과 같은 분류군으로 묶이는 것을 확인 할 수 있었다. - 이러한 결과 ArK 유전자 서열은 계통분류학적 연구에 유용하게 활용될 수 있음을 확인할 수 있었다.
Arginine kinase gene belongs to phosphagen super family and is one of the transferases such as glycocyamine kinase (GK), lombricine kinase (LK), taurocyamine kinase (TK) and creatine kinase (CK) which are expressed in inverterbrates. Arginine kinase (ArK) is commonly called arginine phosphokinase, adenosine 5'-triphosphate, L-arginine, N-phosphotransferase and plays an important role in transporting intracellular energy and regulating phosphoric acid concentration. ArK is also known to play a critical role in arginine and proline metabolism. In this study, we conducted transcriptomic analysis of Mirus junensis using the Next Generation Sequencer and all the sequences containing arginine kinase were obtained by using BLASTx against NCBI NR database and PANM database. We confirmed 358 amino acid residues and 1,074 bp coding region of ArK gene. Using BLAST analysis, we collected 14 similar sequences of M. junensis ArK. Analsysis of dendrogram derived from multiple sequence alignment using clustalW engine of MEGA 7 program showed that it is most similar to Nesiohelix samarangae and belongs to Mollusk with Aplysia kurodai, Conus ebraeus. The ArK of M. junensis needs further investigation about immune system and physiological study.
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