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ISSN : 1225-3480
Optimized protocols of cold shock treatment for the induction of triploidy were developed in Pacific abalone Haliotis discus hannai. Time windows of polar body extrusions were analyzed and various technical parameters including initial treatment times, treatment temperatures (－1ºC, 1ºC and 3ºC) and treatment durations (10 to 24 min) were tested in order to develop the best combination(s) among parameters enabling the efficient blocking the polar bodies. Based on a series of tests regarding hatchability of embryos, normality of larvae and induction efficiency of triploidy, the most appropriate protocol to block the first polar body was proven to be the cold shock treatment at 1ºC for 14 min with an initial treatment at 11 min post insemination. On the other hand, the most efficient condition for blocking the second polar body was the cold shock at 1ºC for 14 min with an initial treatment at 25-27 min post insemination. Under these treatment conditions, larval yields of cold-shock treated groups relative to non-treated control groups were up to 70% with a fairly consistent triploidy rate up to near 100%. Efficacy and reproducibility of triploid induction were further validated under scaled-up conditions; however, the yield of triploid larvae was highly influenced depending on the gamete quality used. Results from this study could be a useful basis for developing the practical protocol for mass production of triploid seedlings in this abalone species.
Arai, K. (2001) Genetic improvement of aquaculture finfish species by chromosome manipulation techniques in Japan. Aquaculture, 197: 205-228.
Arai, K., and Okumura, S.I. (2013) Aquaculture-oriented genetic researches in abalone: current status and future perspective. African Journal of Biotechnology, 12: 4044-4052.
Arai, K., Naito, F., and Fujimo, K. (1986) Triploidization of the Pacific abalone with temperature and pressure treatments. Bulletin of the Japanese Society for the Science of Fish, 52: 417-422.
Beaumont, A.R., and Fairbrother, J.E. (1991) Ploidy manipulation in molluscan shellfish: a review. Journal of Shellfish Research, 10: 1-18.
Dheilly, N.M., Jouaux, A., Poudry, P., Favrel, P., and Lelong, C. (2014) Transcriptomic profiling of gametogenesis in triploid Pacific oysters Crassotrea gigas: towards an understanding of partial sterility associated with triploidy. PLoS One, 9: 2112094.
Elliott, N.G. (2000) Genetic improvement programmes in abalone: what is the future? Aquaculture Research, 31: 51-59.
Felip, A., Zanuy, S., Carrillo, M., and Piferrer, F. (2001). Induction of triploidy and Gynogenesis in teleost fish with emphasis on marine species. Genetica, 111:175-195.
Jee, Y.-J., and Chang Y.J. (1995) Induction of triploid abalone Haliotis discus hannai and its biological characteristics. I. Induction of triploid abalone. Journal of Aquaculture, 8: 159-170.
Komen, H., and Thorgaard, G.H. (2007) Androgenesis, gynogenesis and the production of clones in fishes: a review. Aquaculture, 269: 150-173.
Lee, S.Y., and Nam Y.K. (2016) Transcriptional responses of metallothionein gene to different stress factors in Pacific abalone (Haliotis discus hannai). Fish and Shellfish Immunology, 58: 530-541.
Lee, S.Y., and Nam, Y.K. (2017) Molecular cloning of metal-responsive transcription factor-1 (MTF-1) and transcriptional responses to metal and heat stresses in Pacific abalone Haliotis discus hannai. Fisheries and Aquatic Sciences, 20: 9.
Li, Y., Li, X., and Qin J.G. (2007) Triploidy induction in Australian greenlip abalone Haliotis laevigata (Donovan) with cytochalasin B. Aquaculture Research, 38: 487-492.
Liu, W., Heasman, M., and Simpson, R. (2004)Optimization of triploidy induction in the blacklip abalone, Haliotis rubra (Leach, 1814), using 6-dimethylaminopurine. Aquaculture Research, 35:1076-1085.
Maldonado, R., Ibarra, A.M., Ramirez, J.L., Vasquez, J.E., Badillo, L.M. (2001) Induction of triploidy in Pacific red abalone (Haliotis rufescens). Journal of Shellfish Research, 20: 1071-1075.
Nam, Y.K., Choi, G.C., Park, D.J., and Kim, D.S. (2001)Survival and growth of induced tetraploid mud loach. Aquaculture International, 9: 61-71.
Okumura, S.-I., Arai, K., Harigaya, Y., Eguchi, H., Sakai, M., Senbokuya, H., Furukawa, S., and Yamaori K. (2007) Highly efficient induction of triploid Pacific abalone Haliotis discus hannai by caffeine treatment. Fisheries Science, 73: 237-243.
Park, C.-J., and Kim, S.Y. (2013) Abalone aquaculture in Korea. Journal of Shellfish Research, 32: 17-19.
Peruzzi, S., Kettumen A., Primicerio R., and Kauric, G. (2007) Thermal shock induction of triploidy in Atlantic cod (Gauds morhua L.). Aquaculture Research, 38: 926-932.
Piferrer, F., Beaumont, A., Falguiere, J.-C., Flajshans, M., Haffray, P., and Colombo, L. (2009) Polyploid fish and shellfish: production, biology and application to aquaculture for performance improvement and genetic containment. Aquaculture, 293: 125-156.
Rasmussen, R.S., and Morrissey, M.T. (2007)Biotechnology in aquaculture: transgenics and polyploidy. Comprehensive Reviews in Food Science and Food Safety, 6: 2-16.
Ryu, J.H., Lee, S.Y., Nam, Y.K., and Gong, S.P. (2018)Non-lethal method for the preparation of metaphase spreads using cultured mantle tissue from live adult abalone. Biotechniques, 65: 33-36.
Wadsworth, P., Wilson, A.E., and Walton, W.C. (2019) A meta-analysis of growth rate in diploid and triploid oysters. Aquaculture, 490: 9-16.
Zhang, G., Wang, Z., Chang, Y., Song, J., Ding, J., Wang, Y., and Wang, R. (1998) Triploid induction in Pacific abalone Haliotis discus hannai Ino by 6-dimethylaminopurine and the performance of triploid juveniles. Journal of Shellfish Research, 17:783-788.