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ISSN : 1225-3480
In order to determine optimal larval rearing conditions, the growth and the survival (%) of larvae on rearing conditions (water temperature, salinity, rearing density) and live food for keen's gaper Tresus keenae were investigated. Live food was used by cultured three microalgal species (Isochrysis galbana, Chaetoceros gracilis, Tetraselmis tetrathele) and microalgae concentrates (Shellfish diet 1800®, Reed Mariculture Inc., San Jose, CA, USA). The fastest growth was observed at 25℃ and the survival rate was over 30% at 15℃ and 20℃. The fastest growth and high survival of larvae were observed at 30 psu. The rearing density of larvae showed the fastest growth and the highest survival at 5 inds./mL. In the optimum rearing conditions of larvae the water temperature was 20℃, salinity was 30-35 psu and the rearing density of larvae was below 5 inds./mL. Larvae fed on different concentrations of cultured microalgae showed the fastest growth at 3 × 104 cells/mL/day and the highest survival at 1 × 104 cells/mL/day. Larvae fed on cultured microalgal species and microalgae concentrates showed the fastest growth and the highest survival at CM100 (cultured microalgae 100%) but the late growth at IA100 (instant algae 100%). Larvae fed on different feeding concentrations of microalgae concentrates showed the fastest growth at 2 × 104 cells/mL/day and the high survival at 1 × 104 cells/mL/day. Thus, larvae showed the fast growth and the high survival fed on microalgae concentrates or cultured microalgal species mixed instant algae. The optimal concentration of microalgae concentrates was 1 × 104 － 2 × 104 cells/mL/day for elevating the growth and the survival for larval rearing of T. keenae.
Choi, J.H., Shin, T.S. and Ahn, C.B. (2005) Nutrient components in the siphon of the surf clam Tresus keenae. Journal of Fish Science and Technology, 8:43-50.
Chu, F.L.E. and Webb, K.L. (1984) Polyunsaturated fatty acids and neutral lipids in developing larvae of the oyster Crassostrea virginica. Lipids, 19: 815-820.
Davis, H.C. and Calabrese, A. (1964) Combined effects of temperature and salinity on development of eggs and growth of larvae of Mercenaria mercenaria and Crassostrea virginica. Journal of Fish Biology, 63:643-655.
Duncan, D.B. (1955) Multiple-range and multiple F tests. Biometrics, 11: 1-42.
Duy, N.D.Q., Francis, D.S., Pirozzi, I. and Southgate, P.C. (2016) Use of micro-algae concentrates for hatchery culture of sandfish, Holothuria scabra. Aquaculture, 464: 145-152.
Duy, N.D.Q., Francis, D.S. and Southgate, P.C. (2017)The nutritional value of live and concentrated micro-algae for early juveniles of sandfish, Holothuria scabra. Aquaculture, 473: 97-104.
Epifanio, C.E., Valenti, C.C. and Turk, C.L. (1981) A comparison of Phaeodactylum tricornutum and and Thalassiosira pseudonana as foods the oyster, Crassostrea virginica. Aquaculture, 23: 347-353.
Haven, D.S. and Morales-Alamo, R. (1970) Filtration of particles from suspension of the American oyster Crassostrea virginica. The Biological Bulletin by Marine Biological Laboratory, Woods Hole, Mass., 139: 248-264.
Helm, M.M. and Millican, P.F. (1977) Experiments in the hatchery rearing of Pacific oyster larvae (Crassostrea gigas Thunberg). Aquaculture, 11: 1-12.
Herrero, C. and Lid, A. (1991) Yields in biomass and chemical constituents of four commercially important marine microalgae with different culture media. Aquaculture Engineering, 10: 99-110.
Kang, H.S. and Kim, C.W. (2018) Spawning and larval developments of the surf clam, Tresus keenae. Korean Journal of Malacology, 34: 9-15.
Kim, J.H., Kim, D.H., Yoo, M.S. and Yang, M.H. (2005)Ultrastructure of gametogenesis of the surf clam (Tresus keenae) (Mactridae: Bivalvia). Journal of the Korean Fisheries Technology, 38: 94-99.
Kim, D.H., Lim, H.K, Min, K.S, Chang, Y.J. and Kim, T.I. (1999) Reproductive cycle of surf clam (Tresus keenae) in southern coast of Korea. Journal of the Korean Fisheries Society, 32: 659-663.
Kim, T.Y. (2006) Studies on the artificial seedling production of the hard clam Meretrix lusoria (Röding). Ph.D. thesis, Pukyong National University, 114pp.
Kim, T.I., Ko, C.S., Hur, Y.B., Yang, M.H. and Chang, Y.J. (2011) Growth and survival of the hard clam, Meretrix petechialis (Lamarck) larvae to food organisms. Korean Journal of Malacology, 27:175-180.
Langdon, C.J. and Waldock, M.J. (1981) The effect of algal and artificial diets on the growth and fatty acid composition of Crassostrea gigas. J. Mar. Biol. Ass. UK, 61: 431-448.
Lee, C.S., Park, Y.J., Hong, K.E. and Kim, Y.D. (1999a)Influence of water temperature and stocking density on the growth and survival rate of geoduck clam, Panope japonica larvae. Bulletin of National Fisheries Research and Development Institute, 55:113-121.
Lee, J.Y., Kim, Y.K. and Lee, C.S. (2011) Growth and survival of the brackish water clam, Corbicula japonica larvae according to rearing conditions. Korean Journal of Malacology, 27: 337-343.
Lid, A., Abalde, J. and Herrero, C. (1992) High yield mixotrophic cultures of the marine microalga Tetraselmis suecica (Kylin) Butcher (Prasinophyceae). Journal of Applied Phycology, 4: 31-37.
Loosanoff, V.L. (1950) Rate of water pumping and shell movements of oyster in relation to temperature (Abstract). Amat. Rec., 108: 620.
Loosanoff, V.L. and Davis, H.C. (1963) Rearing of bivalve molluscs. Advances in Marine Biology, 1:1-136.
Min, B.H. and Shin, H.J. (2010) Effects of rearing condition and species of microalgae on growth and survival of larvae of the sunray surf clam, Mactra chinensis. Korean Journal of Malacology, 26: 303-310.
O'Connor, W.A. and Heasman, M.P. (1998) Ontogenetic changes in salinity and temperature tolerance in the doughboy scallop, Mimachlamys asperrima. Journal of Shellfish Research, 17: 89-95.
Rha, S.J. (2005) Reproductive ecology and induction of settlement of keen's gaper Tresus keenae (Kroda &Habe). Ph.D. thesis, Yosu National University, 134pp.
Shin, Y.K. and Yang, M.H. (2005) Effects of temperature and salinty on the survival and metabolism of Tresus keenae (Mollusca: Bivalvia). Journal of Fish Science and Technology, 8: 161-166.
Southgate, P.C., Beer, A.C. and Ngaluafe, P. (2016)Hatchery culture of the winged pearl oyster, Pteria penguin, without living micro-algae. Aquaculture, 451: 121-124.
Southgate, P.C., Braley, R.D. and Militz, T.A. (2017)Ingestion of micro-algae concentrates by veliger larvae of the giant clam, Tridacna noae. Aquaculture, 473: 443-448.
Walne, P.R. (1974) Culture of bivalve molluscs. Whitefriars Press Ltd., London and Tondridge, 173pp.
Wassnig, M. and Southgate, P.C. (2016) The effects of stocking density and ration on survival and growth of winged pearl oyster (Pteria penguin) larvae fed commercially available micro-algae concentrates. Aquaculture Reports, 4: 17-21.
Web, K.L. and Chu. F.L.E. (1983) Phytoplankton as a food source for bivalve larvae. In: Pruder, G.D., Langdon, C., Conklin, D. (Eds.), Proceedings of the 2nd International Conference of Aquaculture Nutrition: Biochemical and Physiological Approaches to Shellfish Nutrition. World Mariculture Society Special Publication, 2: 272-291.
大橋裕, 山本翠. (1986) ミルクイガイ親貝畜養試驗. 山口縣內海栽培漁業センタ報告, 52-97.
大橋裕, 山本翠. (1986) ミルクイガイTresus keenae KURODA et HABEの增殖に關する硏究-昭和57年度までの槪況. 山口縣內海栽培漁業センタ報告, 55-71.
山本翠, 大橋裕. (1987) ミルクイガイ中間育成試驗. 山口縣內海栽培漁業センタ報告, 61-65.
秋山展示. (1966) ミルクイガイ幼生の水槽飼育について. 水産增殖, 14: 151-156.