ISSN : 1225-3480
In order to determine optimal larval rearing conditions, the growth and the survival (%) of larvae on rearing conditions (water temperature, salinity, rearing density) and live food for keen's gaper Tresus keenae were investigated. Live food was used by cultured three microalgal species (Isochrysis galbana, Chaetoceros gracilis, Tetraselmis tetrathele) and microalgae concentrates (Shellfish diet 1800®, Reed Mariculture Inc., San Jose, CA, USA). The fastest growth was observed at 25℃ and the survival rate was over 30% at 15℃ and 20℃. The fastest growth and high survival of larvae were observed at 30 psu. The rearing density of larvae showed the fastest growth and the highest survival at 5 inds./mL. In the optimum rearing conditions of larvae the water temperature was 20℃, salinity was 30-35 psu and the rearing density of larvae was below 5 inds./mL. Larvae fed on different concentrations of cultured microalgae showed the fastest growth at 3 × 104 cells/mL/day and the highest survival at 1 × 104 cells/mL/day. Larvae fed on cultured microalgal species and microalgae concentrates showed the fastest growth and the highest survival at CM100 (cultured microalgae 100%) but the late growth at IA100 (instant algae 100%). Larvae fed on different feeding concentrations of microalgae concentrates showed the fastest growth at 2 × 104 cells/mL/day and the high survival at 1 × 104 cells/mL/day. Thus, larvae showed the fast growth and the high survival fed on microalgae concentrates or cultured microalgal species mixed instant algae. The optimal concentration of microalgae concentrates was 1 × 104 － 2 × 104 cells/mL/day for elevating the growth and the survival for larval rearing of T. keenae.
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