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ISSN : 1225-3480
Winter mortality of blood cockles (Tegillarca granosa) is a one of the main concerns of cockle aquaculture in Korea. Thus, this study investigated the physiological alternation of the cockles when they were placed in abnormally cold temperature. For this purpose, cockles were exposed to temperatures of 4°C (control), 2°C, 0°C, –2°C, and –5°C for four hours/day over eight days, and the condition index, nitric oxide concentration, and mortality rates were measured after each cold treatment. The results showed that the cockle nitrite concentration increased significantly in all groups, except for the control group, from the 1st day of exposure, and that this NO increase continued until the end of the experiment. In addition, cockles exposed to –5°C for 5 days showed an increase of mortality rate. These results suggested that cockles were experienced a physiological stress when they were exposed to < 2°C and might have resulted in mortality under severe hypothermic condition in winter. Our study also suggested that the measured NO concentration in the hemocytes of cockles is an efficient biomarker for diagnosing physiological stress in cockles exposed to cold shock.
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