open access
메뉴
open access
메뉴
ISSN : 1225-3480
Clithon retropictus is a small gastropod belonging to the Neritidae, and inhabits gravel areas in brackish waters. It has been designated as an endangered species as class II, because of its habitat conditions and reckless development of coastlines. In September 2021, C. retropictus registered in NCBI has only 80 nucleotides, 52 proteins, and 74 genes. In this context, we sequenced the C. retropictus cDNA library using the Illumina Hiseq 2500 platform. Arginine Kinase (AK) sequence was obtained by using BLAST program. To investigate the molecular phylogenetic analysis of C. retropictus, a dendrogram was generated using AK gene as a marker. Based on the phylogenetic tree, it is classified into three classes (Cephalopods, Gastropods, and Bivalvia). In addition, analysis of AK 2D structure using 38 species shows that no striking similarity was found. In conclusion, AK gene of C. retropictus consisted of 1,053 bp (351 amino acids) and could be utilized for molecular phylogenetic classification.
Downs, M., Johnson, P., and Zeece, M. (2016) Insects and their connection to food allergy Insects as sustainable food ingredients. Elsevier, pp 255-272
Grabherr, M.G., Haas, B.J., Yassour, M., Levin, J.Z., Thompson, D.A., Amit, I., Adiconis, X., Fan, L., Raychowdhury, R., and Zeng, Q. (2011) Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nature biotechnology, 29:644-652.
Ji Eun Jeong, and Lee, Y.S. (2013) Identification, sequence characterization and expression analysis of the arginine kinase gene in response to laminarin challenge from the Oriental land snail, Nesiohelix samarangae. Department of Life Science, Soonchunhyang University, 29: 171-179.
Joshi, N., and Fass, J. (2011) Sickle: A sliding-window, adaptive, quality-based trimming tool for FastQ files (Version 1.33)[Software]
Kang, S.W., Park, S., Hwang, H., Chung, J., Sang, M., Min, H., Park, J., Cho, H., Patnaik, B., and Lee, Y. (2019) PANM DB ver 3.0: an update of the bioinformatics database for annotation of large datasets from sequencing of species under Protostomia clade. Korean Journal of Malacology, 35:73-75.
Kumar, S., Stecher, G., Li, M., Knyaz, C., and Tamura, K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol. Biol. Evol., 35: 1547-1549.
Madeira, F., Park, Y.M., Lee, J., Buso, N., Gur, T., Madhusoodanan, N., Basutkar, P., Tivey, A.R., Potter, S.C., and Finn, R.D. (2019) The EMBL-EBI search and sequence analysis tools APIs in 2019. Nucleic acids research, 47: W636-W641.
Martin, M. (2011) Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet. journal. 17: 10-12.
McGuffin, L.J., Bryson, K., and Jones, D.T. (2000) The PSIPRED protein structure prediction server. Bioinformatics, 16: 404-405.
Min, R., Hee Ju Hwang, Jong Min Chung, Min Kyu Sang, Hang Chul Cho, Jie Eun Park, Ki Yoon Jung, Hong Seog Park1, Han2, Y.S., and Lee, a.Y.S. (2018)Molecular Phylogenetic studies of Mirus junensis using Arginine kinase gene sequence. The Korean journal of malacology, 34: 107-114.
Park, J., Min Kyu Sang, Hee-Ju Hwang, Dae Kwon Song, Jun Yang Jeong, So Young Park, Se Won Kang, Hong Seog Park, Yeon Soo Han, Lee, J.S., and Lee, a.Y.S. (2020) Molecular Phylogenetic Analysis of Ellobium chinense Using Arginine Kinase Korean J. Malacol, 36(3): 133-141.
Pertea, G., Huang, X., Liang, F., Antonescu, V., Sultana, R., Karamycheva, S., Lee, Y., White, J., Cheung, F., Parvizi, B., Tsai, J., and Quackenbush, J. (2003)TIGR Gene Indices clustering tools (TGICL): a software system for fast clustering of large EST datasets. Bioinformatics, 19: 651-652.
Rice, P., Longden, I., and Bleasby, A. (2000) EMBOSS:the European molecular biology open software suite. Trends in genetics, 16: 276-277.
Saitou, N., and Nei, M. (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol., 4: 406-425.
Sang, M., Hwang, H., Chung, J., Park, J., Song, D., Jeong, J., Park, S., Park, H., Jo, Y., and Lee, J. (2020) Phylogenetic analysis of endangered wild animal class II Satsuma myomphala using the argrinine kinase gene. Korean Journal of Malacology, 36: 97-104.
Tanaka, K., Ichinari, S., Iwanami, K., Yoshimatsu, S., and Suzuki, T. (2007) Arginine kinase from the beetle Cissites cephalotes (Olivier). Molecular cloning, phylogenetic analysis and enzymatic properties. Insect. Biochem. Mol. Biol., 37: 338-345.
Uda, K., Fujimoto, N., Akiyama, Y., Mizuta, K., Tanaka, K., Ellington, W.R., and Suzuki, T. (2006) Evolution of the arginine kinase gene family. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 1: 209-218.
Voncken, F., Gao, F., Wadforth, C., Harley, M., and Colasante, C. (2013) The phosphoarginine energy-buffering system of trypanosoma brucei involves multiple arginine kinase isoforms with different subcellular locations. PLoS One, 8: e65908.