ISSN : 1225-3480
The complete mitochondrial genome of small sea snail, Chlorostoma lischkei (Tapparone Canefri, 1874) (Gastropoda: Tegulidae) was determined in this study, using Illumina Hiseq sequencing. The mitogenome length was 17,980 bp. Its gene arrangement was similar to that of mitogenome of Tegulids, having 13 protein-coding genes and 2 ribosomal RNAs. However, C. lischkei had 24 tRNA genes with two more tRNAs than others. It had an overall A+T content of 79.7% (27.3% A, 7.5% C, 12.8% G, and 52.4% T). Our results could be used for future taxonomic and evolutionary studies of subclass Vetigastropoda.
About 85,000 species of gastropods are known around the world, and the imports rate about edible gastropods are on the rise every year in Korea. The system will be needed to reflect taxonomic diversity about similar gastropoda groups. Because inaccurate information about edible species can cause serious problems in market. In this study, to cope with this problem, a study was conducted to accurately identify gastropods, and selected six target country with a large imported rate of edible gastropods in Korea. The collected gastropods were classified into 8 orders, 22 families and 33 species, including unrecorded in Korea, and were identified by morphological classification. Sequence analysis of the COI gene was performed for all collected species by COI universal primer. The result of molecular phylogenetic analysis and morphological classification result are similar. As a result of this study, it was confirmed that most gastropoda species can be identified using universal primers for gastropod COI sequences, and will be used as data for species identification of gastropods in the future.
To investigate the habitat environment and distribution of Corbicula japonica, salinity, sediment composition and its densities were investigated in the Seomjin River. Salinity in the study area ranged from 0.08 to 23.18 psu. Sediment composition was sand or silty sand. The sorting values ranged from 1.0 to 2.7 Ø (mean 1.5 Ø), and the water content (%) and ignition loss (%) were higher in the downstream than in the upstream. Density of C. japonica ranged from 2.5 to 33.7 inds./m2. Density near Mokdo (Site 3) was the highest and that near Gojeon (Site 1) and Dugok (Site 8) was low. In addition, a resource survey of C. japonica was conducted to estimate its biomass in 3 times. The total biomass in the Seomjin River was estimated to be 191.1 tons with the biomass of Mokdo (Site 3) the highest.
This study examined the physiological responses of cockles exposed to thermal stress in order to optimize techniques for measurement of physiological alternations in cockles and understand the importance of thermal stress on summer mortality events. For these reasons, cockles acclimated to 25°C were exposed to 30°C, 35°C, or 40°C for 4h/day over eight days, and the condition index, nitric oxide (NO) concentration, burrowing rates and mortality rates were measured after each heat treatment. From the 1st day of exposure, and each day after, NO concentrations in cockles significantly increased when the temperatures was > 30°C. In contrast, decreases in phagocytosis rates were observed when specimens were exposed to 30°C from the 2nd day of exposure while the burrowing rates started to decline from the 6th day of exposure when exposed to > 35°C. In addition, the mortality rate of cockles exposed to 35°C for 5 days increased. This study confirmed that cockles exposed to temperatures above 30°C may experience physiological stress, possibly leading to death, depending on the exposure time.
The PANM database (Protostome DB) was first established in 2015 as a public database platform aimed at the efficient annotation of next-generation sequencing (NGS) data of species belonging to Mollusks. It was updated to version 2 in 2016 and version 3 established in 2019, with a total of 11,615,243 protein sequences. The PANM DB version 4 was updated by integrating the protein sequences of Arthropoda, Nematoda, and Mollusca registered in NCBI from the update to PANM DB v3 until February 2021. The PANM DB v4 accounts for only about 4% of the NCBI-nr database but significantly reduces the Invertebrates’ NGS data annotation time.