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ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

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새만금 주변 갯벌 이매패류 개체군의 서식밀도와 각장크기 조성
최진우(서울대학교 해양연구소) ; 권봉오(군산대학교) ; 윤서준(서울대학교 해양연구소) ; 남정호(한국해양수산개발원) ; 김종성(서울대학교 해양연구소) pp.167-175
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Abstract

This study was focused to know what happened to three bivalve resources (Ruditapes philippinarum, Mactra veneriformis, and Meretrix petechialis) at tidal flats around the Saemangeum during recent years. The population density of M. petechialis at the Yubu-do tidal flat has been gradually declined by the continuous harvesting of fishermen and reached to be a very low density of 0.6 ind./m2 . Because a very low juvenile recruitment is expected due to the lack of larval supply from a very low fertilization rate due to its low adult density, it is necessary to provide some protective sites containing dense adults of M. petechialis in order to avoid the Allee effects in Yubu-do tidal flat. In the case of the populations of R. philippinarium and Mactra veneriformis at Wi-do tidal flat, there was no natural recruitment during recent two years and few small individuals of these two species and only large individuals were found compared with those from Yubu-do and Sinshi-do, especially at the lower tidal flat due to the accumulation of fine sediments. For Ruditapes and Mactra populations, it should be necessary for a detailed a detailed monitoring study to solve problems on the survival of juveniles and recruitment failure and to know whether there are some relationships between the high mortality and recruitment failures and fine particle depositions at the tidal flats.

강우 영향에 의한 육상오염원이 자란만ㆍ사량도해역의 해수 및 굴 (Crassostrea gigas) 에 미치는 영향평가
남기호(국립수산과학원 남동해수산연구소) ; 정상현(국립수산과학원 연구기획과) ; 윤민철(국립수산과학원 남동해수산연구소) ; 김지훈(국립수산과학원) ; 조성래(국립수산과학원 남동해수산연구원) ; 이장원(국립수산과학원 남동해수산연구소) ; 하광수(국립수산과학원 남동해수산연구소) pp.177-188
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Abstract

In this study, we evaluated the effect of inland pollution sources on sea water and oyster (Crassostrea gigas) in JaranmanㆍSaryangdo area after rainfall events. We analyzed the sanitary indicator microorganism such as coliform group, fecal coliform and Escherichia coli (E. coli) in the discharge water of major inland pollutants, sea water and oyster for 3 days after 24.5, 50.0 and 67.5 mm rainfall events. As the rainfall in this area became increased, the range of fecal coliform of 15 contaminants was increased gradually (24.5 mm; 4.5-54,000 MPN/100mL, 50.0 mm; < 1.8-92,000 MPN/100mL, 67.5 mm; 49-240,000 MPN/100mL). However, the range of radius of impacted area of 15 contaminants was decreased and getting better for 3 days. The peak pollution was observed in the Yongho-village domestic waste water. The calculated impact area of inland pollution sources was 0.03 km2 immediately after 24.5 mm rainfall and expanded to 0.2 km2 after 67.5 mm rainfall. These calculations of impacted area matched results from fecal coliform analyses with sea water. According to the evaluation of the influences of inland pollution sources and fecal coliform analyses on sea water and oyster this area, pollutants from inland sources did not reach the boundary line of the oyster growing area after rainfall events. The bacteriological water quality of the oyster growing this area met the Korea standard and US NSSP requirements for approved oyster growing area. And the E. coli level of oyster at 10 station in the designated area was met the EU regulation and the limit of raw oyster in Korea with ranged from < 18 to 230 MPN/100g.

부남호 저층수 및 수온과 염분에 대한 바지락 영향 평가
서진영(한국해양과학기술원 선박평형수연구센터) pp.189-193
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Abstract

This study was conducted to measured the survival rate (or mortality) of Ruditapes philippinarum according to the change of salinity when the water temperature is high. In laboratory, the mortality test was performed on the R. philippinarum, we conducted experiments at water temperature 20, 25, and 30℃, salinity was tested at intervals 5 psu from 0 to 35 psu. As a result of exposure for bottom water of Bunam lake, the mortality was 20% in 100% bottom water, and it was found that there was no effect at concentrations below 50% in bottom water in Bunam lake. The LC50 by water temperature was 4.816 psu at 20℃, 14,645 psu at 25℃, and 20.063 psu at 30℃. The death of the clam was observed only at a concentration of 15 psu or less at 20℃. But the dead R. philippinarum observed at all concentrations of salinity at 30℃. The filtration rate was 0 ml min-1 at only 0 and 5 psu at 20℃, but the feeding activity started from 20 psu or higher at 30℃. As such, exposure to low-salinity water during the high-temperature period has an effect on the filtration rate and mortality of R. philippinarum.

대맛조개 (Solen grandis) 의 수정란 발생과 유생 성장
최보현(전라남도 해양수산과학원) ; 이동근(전라남도 해양수산과학원) ; 박충국(전라남도 해양수산과학원) ; 황남용(전라남도 해양수산과학원) ; 안신홍(전라남도 해양수산과학원) ; 강요한(전라남도 해양수산과학원) ; 고경동(전라남도 해양수산과학원) ; 이지수(전라남도 해양수산과학원) ; 이경식(전라남도 해양수산과학원) ; 박준택(전라남도 해양수산과학원) ; 이경우(전라남도 해양수산과학원) pp.195-201
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Abstract

Solen grandis is an industrially important edible bivalve that is traded at high prices as it is unfarmed and the production has decreased due to environmental pollution and overfishing. Major studies on Solen grandis have reported only basic research on reproductive development, change, etc., and no studies on spawning, egg development, and larval management have been reported for seed production and aquaculture technology development. Therefore, in this study, spawning was carried out using mature plaques secured through habitat survey for Solen grandis seed acid, and artificial seed production was succeeded, and the egg development process from fertilized egg to D phase larva and larval development process of 1 mm or more were observed through a microscope. Additionally, this study aims to develop technology for promoting seed production as basic research for fostering high value-added bivalve industrial varieties and resource recovery. Based on the existing research data, we conducted research on the development of fertilized eggs and larval development using the mature plaques of Solen grandis.

선발 5세대 북방전복 성장형질의 유전모수 및 선발효과 추정
김혜진(국립수산과학원 수산종자육종연구소) ; 양혜림(국립수산과학원 어류육종연구센터) ; 박종원(국립수산과학원 어류육종연구센터) pp.203-210
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Abstract

This study was conducted for the purpose of estimating the genetic improvement and effects of selection on growth traits of Pacific Abalone, Haliotis discus hannai. For the analysis, 2,733 individuals of 5th generation of selective breeding produced in 2017, their pedigree data and phenotypic data of growth traits at the age of 30-months. Genetic parameter, heritability, and breeding value were estimated by the Best linear unbiased prediction (BLUP) method using REMLF90, with sex treated as a fixed effect. At the age 30-months after hatching, the overall means of shell length, shell width, and total weight were 88.3 mm, 57.8 mm and 79.4 g, respectively. and the coefficient of variation of weight was 22.6%, showing the greatest variation among the three growth traits. A high level of significance was recognized as a result of ANOVA of growth traits for sex (P < 0.001). The heritability of shell length, shell width, and total weight were 0.315, 0.339, and 0.304, respectively. The higher the selection intensity, the higher the expected genetic improvement and selection effect. However, in order to minimize inbreeding while maintaining the genetic diversity of the population, selection intensity should be appropriately considered.

Duplex PCR을 이용한 재첩과 일본재첩 간 신속한 종 판별법 개발
김용휘(순천향대학교) ; 윤봉한(순천향대학교) ; 한호섭(순천향대학교) ; 김혜진(순천향대학교) ; 방인철(순천향대학교) pp.211-220
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Abstract

A duplex PCR primer set was developed for molecular biological species identification by making species-specific primers for Corbicula fluminea and Corbicula japonica distributed in Korea. The developed species-specific primers were designed to form an interspecies-specific band around C. fluminea 289 bp and C. japonica 143 bp by searching for a site of interspecies genetic variation appearing on the nucleotide sequence of the cytochrome c oxidase subunit 1 (co1) gene of mitochondrial DNA. A verification experiment was also performed on the duplex PCR primer set by measuring the amplification efficiency for each repeat number and genomic DNA (gDNA) concentration. The duplex PCR primer set finally established was found to require a minimum of 10 ng/μL of gDNA concentration when considering the sample amount and PCR reaction cycle in terms of economic feasibility, and 30 cycles were found to be suitable. Therefore, the duplex PCR primer set between C. fluminea and C. japonica developed in this study is judged to be able to perform accurate species identification quickly and conveniently by PCR band observation.

생물정보학을 활용한 멸종위기 연체동물 전사체 서열에 오염된 곰팡이 유전자 발굴
송대권(순천향대학교 생명과학과) ; 박현진(한국방송통신대학교 농학과) ; 상민규(생명자원 바이오빅데이터 분석 및 활용 연구지원센터) ; 박지은(순천향대학교 생명과학과) ; 정준양(순천향대학교 생명과학과) ; 홍찬의(순천향대학교 생명과학과) ; 김용태(순천향대학교 생명과학과) ; 신현준(순천향대학교 생명과학과) ; 류자미(순천향대학교 생명과학과) ; 조용훈(순천향대학교 생명과학과) ; 한연수(전남대학교) ; 이용석(순천향대학교 생명과학과) ; 장종수(한국방송통신대학교) pp.221-233
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Abstract

The amount of data is growing very fast as advances in NGS technology enable the acquisition of large amounts of genome and transcriptome data. Moreover, the accuracy and speed of bioinformatic analysis of NGS data remains of great importance these days. However, the sequence database of mollusks is fall short of other organisms groups, and it thus appears that the annotation results after BLAST analysis are not accurate and reliable due to potential contamination with fungal sequences in mollusks sequence database. In this context, we constructed a BLAST database with 20 species of mollusk unigene sequences and 32 species of fungal sequences derived from previous studies. In order to confirm the contamination of fungal gene sequences in the unigenes of 20 endangered species, bioinformatics analysis was performed using BLAST. It reveals that the NGS sequences of mollusks are mixed with fungal sequences. Taken together, our results suggest that it is essential to reconfirm mollusks sequence information before publication.

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