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ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

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한국산 달팽이속 집단의 계통분류학적 연구
김강산(국립생태원멸종위기종복원센터곤충·무척추동물연구팀) ; 유인성(국립생태원멸종위기종복원센터곤충·무척추동물연구팀) ; 이준상(순천향대학교한국자생동물자원활용융복합연구소) pp.73-79 https://doi.org/10.9710/kjm.2023.39.3.73
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Abstract

The Korean Acusta specimens were collected from six sites: Seo-gu, Incheon; Yeongyang, Gyeongsangbuk-do; Hongdo Island, Jeollanam-do; Jodo Island, Jeollanam-do; Chuncheon, Gangwon-do; and Seogwipo, Jeju-do. It was observed that the morphological characteristics follow the typical genus Acusta. In order to confirm their phylogenetic position, DNA analyses were conducted targeting three regions, cytochrome c oxidase subunit 1 (CO1), 16S ribosomal RNA (16S), and internal transcribed spacer 2 (ITS2). As a result, the Korean population of Acusta specimens were confirmed to be of the same species. In the phylogenetic trees, it was grouped in the same clade as Acusta redfieldi (Reeve, 1852) and showed the lowest variation of Acusta group. It was also the second most closely related to A. siebolditina (L. Pfeiffer, 1850). Therefore, the Korean Acusta is regarded as A. redfieldi and showed closer genetic similarity to the Chinese population than to the Japanese population. In addition, in this study, the nucleotide sequences of the Korean group Acusta are recorded in a public database for the first time, and then a photograph of the individual and a brief morphological description are provided.

경남 남해군 진주만에 서식하는 종밋 (Arcuatula senhousia) 의 분포와 번식주기
이희중(국립수산과학원 남동해수산연구소,) ; 조재권(국립수산과학원 남해수산연구소) ; 김현중(국립군산대학교 수산생명의학과) ; 박경일(국립군산대학교 수산생명의학과) pp.81-88 https://doi.org/10.9710/kjm.2023.39.3.81
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Abstract

Arcuatula senhousia, a bivalve mollusk, exerts a significant negative impact on ecological aspects within the realm of bivalve aquaculture. This study delved into the reproductive cycle and distribution of A. senhousia in Jinju Bay, Korea, spanning from February to December 2020. The species inhabiting Jinju Bay displayed a dense concentration in the central part of the bay, where shellfish aquaculture facilities are prevalent. However, they experienced a complete die-off in August, and no specimens were collected thereafter until the conclusion of the experiment. The reproductive cycle of A. senhousia, collected from February to July 2020, revealed that 13.6% were females, while 56% were males in the early developmental stage among the specimens collected in February. Males exhibited a more rapid maturation of reproductive organs. Gonadal maturation was observed in both male and female specimens starting in May, with spawning occurring from May to July. The mortality of A. senhousia observed in August was attributed to underwater hypoxia or anoxic conditions. The insights into the reproductive cycle of A. senhousia inhabiting the Jinju Bay area are anticipated to hold value for the development of techniques in shellfish aquaculture management.

강우 발생에 따른 육상 오염원이 고성만의 해수 및 패류에 미치는 영향 평가
김지훈(국립수산과학원 남동해수산연구소) ; 윤민철(국립수산과학원 남동해수산연구소) ; 조성래(국립수산과학원 남동해수산연구소) ; 이장원(국립수산과학원 남동해수산연구소) ; 정상현(국립수산과학원 연구기획과) ; 남기호(국립수산과학원 기후변화연구과) ; 하광수(국립수산과학원 식품안전가공과) ; 박큰바위(국립수산과학원 남동해수산연구소) pp.89-101 https://doi.org/10.9710/kjm.2023.39.3.89
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Abstract

In this study, we evaluated the effect of inland pollution sources on seawater and shellfish (Oyster and Scallop) in Goseong bay after rainfall events. We analyzed the sanitary indicator microorganism such as total coliform, fecal coliform and Escherichia coli (E. coli) in the discharge water of major inland pollutants, seawater and shellfish for 3 days after 25.5, 56.0 and 101.5 mm rainfall events. According to these results, the range of total coliform and fecal coliform was < 1.8-2,400 and < 1.8-2,400 after 25.5 mm rainfall and was from < 1.8 to 110 and from < 1.8 to 13 MPN/100 mL after 56.0 mm rainfall and was from 79 to 11,000 and from 79 to 11,000 MPN/100 mL after 101.5 mm rainfall in the discharge water of 2 waste water treatment plants. Also the range of fecal coliform and radius of impacted area of 9 contaminants (stream) was from 13 to 35,000 MPN/100 mL and from 14 to 2,249 m, respectively. The fecal coliform of seawater at 34 stations ranged from < 1.8 to 540 MPN/100 mL, respectively. And The E. coli level of shellfish (Oyster and Scallop) at 6 station ranged from < 18 to 5,400 MPN/100 g.

칠천도 패류생산해역의 해수 및 패류에 대한 위생학적 안전성 평가연구
조성래(국립수산과학원 남동해수산연구소) ; 윤민철(국립수산과학원 남동해수산연구소) ; 김지훈(국립수산과학원 남동해수산연구소) ; 이장원(국립수산과학원 남동해수산연구소) ; 신혜영(국립수산과학원 남동해수산연구소) ; 남기호(국립수산과학원 연구기획과) ; 정상현(국립수산과학원 기후변화연구과) ; 권지영(국립수산과학원 식품안전가공과) ; 하광수(국립수산과학원 식품안전가공과) ; 박큰바위(국립수산과학원 남동해수산연구소) pp.103-112 https://doi.org/10.9710/kjm.2023.39.3.103
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Abstract

The sanitary survey was evaluated for sanitary state of seawater and shellfish in Chilcheondo area from September 2020 to February 2023. The stations of sanitary survey in Chilcheondo area were composed of 28 seawater station, 3 oyster (Crassostrea gigas) and 1 mussel (Mytilus galloprovinciallis). The samples were collected monthly at each station. The range of Fecal coliform, geometric mean and 90th percentile for 840 seawater samples were < 1.8-35,000 MPN/100 mL, < 1.8-3.3 and 1.8-34 MPN/100 mL, respectively and in addition to seawater in the Chilcheondo area satisfied as level of designated area according to Korea criteria and approved area according to US criteria. Also the range of Fecal coliform and E. coli for 88 oyster samples and 30 mussel samples were <18-35,000 and <18-9,200 MPN/100 g, respectively. The bacteriological quallity of shellfish collected from Chilcheondo area meets the standard value based on shellfish hygiene of the Food Sanitation Act of Korea and the Grade B according to the classification of shellfish harvesting areas of European Union.

바지락의 생체지표 유전자 발현과 갯벌어장 퇴적물 건강도 평가
최윤석(국립수산과학원 동해수산연구소) ; 김맹진(국립수산과학원 동해수산연구소) pp.113-120 https://doi.org/10.9710/kjm.2023.39.3.113
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Abstract

In order to assess the health level of tidal flat, we investigated heavy metals of surface sediments and the biomarker genes of Ruditapes philippinarum samples in the west coast of Korea. Manila clams were collected from 8 sites of western coast and analyzed the total RNA of these meat part with RT-qPCR method. We have examined the geochemical characteristics of surface sediments and the concentration of inorganic elements of manila clam with XRF. It is possible for the health level of inhabit organisms using analysis of expression of biomarker genes such as heat shock protein 70 (Hsp70), heat shock protein 90 (Hsp90), glutathione S-transferases (GST) and thioredoxin (TRX). That is related to stress, immune and antioxidant related genes. Results showed that the expression of biomarker genes were changed in the 8 sites and were relevant with the heavy metal concentration of sediments, respectively. We suggested that biomarker genes were played an important role for the health level assessment of tidal flats.

In vitro propagation of the alveolate protozoa Perkinsus olseni isolated from Manila clam Ruditapes philippinarum in Korea
KangHyun-Sil(Department of Marine Life Science (BK21 PLUS) and Marine Science Institute, Jeju National University, 102 Jejudaehakno Jeju 63243, Republic of Korea) ; HongHyun-Ki(Department of Marine Life Science (BK21 PLUS) and Marine Science Institute, Jeju National University, 102 Jejudaehakno Jeju 63243, Republic of Korea) ; ChoiYoung-Ghan(Department of Marine Life Science (BK21 PLUS) and Marine Science Institute, Jeju National University, 102 Jejudaehakno Jeju 63243, Republic of Korea) ; LeeHye-Mi(Department of Marine Life Science (BK21 PLUS) and Marine Science Institute, Jeju National University, 102 Jejudaehakno Jeju 63243, Republic of Korea) ; ChoiKwang-Sik(Department of Marine Life Science (BK21 PLUS) and Marine Science Institute, Jeju National University, 102 Jejudaehakno Jeju 63243, Republic of Korea) pp.121-126 https://doi.org/10.9710/kjm.2023.39.3.121
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Abstract

The alveolate protozoan parasite Perkinsus olseni has a unique life cycle, including a mobile zoospore in an aerobic water column, vegetative trophozoite in the host tissues, and dormant hypnospore in an anaerobic environment such as decomposing host tissues or subsurface of the sediment. In this study, P. olseni trophozoites were induced from the zoospores in vitro using a Dulbecco Modified Eagle’s:Ham’s F-12 (DME/Ham’s F-12, 1:2) fortified with antibiotics and supplemented with 5% fetal bovine serum, 50 mM HEPES buffer, 3.5 mM sodium bicarbonate, and 200 mM L-glutamine. In the growth media, P. olseni zoospores developed into trophozoites and reproduced within two weeks at 25 °C room temperature. During two weeks of culture, the trophozoites increased their cell size from a few microns to 34.4 ± 14.1 μm in diameter. Numerous small-sized daughter cells of the trophozoites could be observed 4 and 6 days after incubation, suggesting that the doubling time of the trophozoites in the media can be 4 to 6 days. The hypnospore stage and subsequent zoosporulation could also be induced from the trophozoite stage developed in the growth media, confirming that the trophozoites are vital, although they were produced in vitro. The Dulbecco’s modified Eagle’s:Ham’s F-12 (DME/Ham’s F-12, 1:2) growth medium was considered a method of choice in the mass production of P. olseni trophozoites in vitro, as previously applied in in-vitro culture of Perkinsus spp.

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