Abstract

We identified nicotine, cotinine and toluene in high school volunteer's urine by using GC/NPD, GC/FID and GC/MS. To analyze of nicotine and cotinine, urine samples were extracted with diethylether and centrifuged on a benchtop centrifuge for 5 min. The upper organic layer was injected into a GC. The distributions of nicotine and cotinine were <TEX>$4{\sim}630{\mu}g/L$</TEX> and <TEX>$63{\sim}1,602{\mu}g/L$</TEX> in smoking-group, respectively. To analyze of toluene, head space vial was filled with 2mL sodium citrate solution and 1mL of urine. The vial was warmed in a water bath at <TEX>$55^{\circ}C$</TEX> for 20min, and then <TEX>$250{\mu}L$</TEX> of head space air was injected into a GC. The result show that toluene was not detected in all of the volunteers' samples. However, the range of toluene was 0.1~28.0mg/L in glue sniffer's urine samples(NISI data).