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  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

Distributions of Nicotine and Toluene in Teenagers' Urine

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1997, v.10 no.1, pp.1-8
Park, Sungwoo
Seo, Baeseck
You, Jaehoon
Kim, Namyee
Shin, Hosang
Han, Wansoo
Kim, Ilkwang
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Abstract

We identified nicotine, cotinine and toluene in high school volunteer's urine by using GC/NPD, GC/FID and GC/MS. To analyze of nicotine and cotinine, urine samples were extracted with diethylether and centrifuged on a benchtop centrifuge for 5 min. The upper organic layer was injected into a GC. The distributions of nicotine and cotinine were <TEX>$4{\sim}630{\mu}g/L$</TEX> and <TEX>$63{\sim}1,602{\mu}g/L$</TEX> in smoking-group, respectively. To analyze of toluene, head space vial was filled with 2mL sodium citrate solution and 1mL of urine. The vial was warmed in a water bath at <TEX>$55^{\circ}C$</TEX> for 20min, and then <TEX>$250{\mu}L$</TEX> of head space air was injected into a GC. The result show that toluene was not detected in all of the volunteers' samples. However, the range of toluene was 0.1~28.0mg/L in glue sniffer's urine samples(NISI data).

keywords
nicotine, cotinine, toluene, glue sniffing


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