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- E-ISSN 2288-8985
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A Study on the Analysis of Histamine by Spectrofluorimetry
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1997, v.10 no.1, pp.43-52
Rhee,
J. S., Kim,
Y. S., Cha,
K. S., Rhee,
H. J., Hong,
C. S., Nam,
H. W., Myung,
C. O., Lee,
K. W., &
Lee,
Y.
C.
(1997). A Study on the Analysis of Histamine by Spectrofluorimetry. Analytical Science and Technology, 10(1), 43-52.
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Abstract
The application of high performance liquid chromatogaphy(HPLC) with fluorimetric detection for the measurement of histamine in biological fluids has been developed recently. Because of time consumption during measurement and difficulties in controling optimum operating condition for HPLC system, spectrofluorimetry was adopted to detect histamine. Detection limit by spectrofluorimetry was <TEX>$3.0{\times}10^{-2}{\mu}g/mL$</TEX> wi th R. S. D. 3.74%~16.2% on standard solution. The relative ratio of histmine levels between the result from HPLC-fluorimetry and that from spectrofluorimetry ranged from 1.5 to 3.5. The recoveries of each samples obtained from standard addition method by spctrofluorimetry have shown 100%~125%.
- keywords
- Histamine, Spectrofluorimetry
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