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Effect of Oxygen Composition on Polyhydroxybutyrate Synthesis by Alcaligenes eutrophus at Various Pressures
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1997, v.10 no.1, pp.75-81
Kim,
K., Shin,
S. K., &
Kwon,
H.
(1997). Effect of Oxygen Composition on Polyhydroxybutyrate Synthesis by Alcaligenes eutrophus at Various Pressures. Analytical Science and Technology, 10(1), 75-81.
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Abstract
Poly-<TEX>${\beta}$</TEX>-hydroxybutyrates(PHB) are well known intracellular biopolymer which are completely degraded to carbon dioxide and water in the landfill. The pressurized fermentation method was used to increase the cell growth of Alcaligenes eutrophus and productivity of PHB. The experimental data were analyzed in terms of carbon source and gas composition effects at various elevated pressures and temperatures. The results reveal that the flow rate of hydrogen gas of 0.0075vvm for the culture is better PHB production than the no hydrogen flow when fructose was used as a sole carbon source. The higher yields and productivities of PHB biosynthesized by A. eutrophus were obtained when the oxygen composition was changed from 2% to 8% at 6atm and <TEX>$30^{\circ}C$</TEX>.
- keywords
- Poly-<TEX>${\beta}$</TEX>-hydroxybutyrates, PHB, Alcaligenes eutrophus, hydrogen, biopolymer
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