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  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

SPF Measurement and Cytotoxicity of Sunscreen Agents in Cosmetic

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1998, v.11 no.2, pp.79-87
Kim, In-Young
Kang, Sam-Woo
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Abstract

Consumers have recently preferred to purchase extensive UV intercepting products, which are waterproof and free from side effects on skin. During the testing of cytotoxicity (in-vitro) in neutral red (NR) method, cell survival ratio of UV-B interceptors decreased to just above 0.08 w/v%, and it was observed that the UV-A interceptors the ratio also decreased to just above 0.06 w/v%. In addition patch-tests of inorganic UV interceptors resulted in no skin irritation even below 10.0 and 11.25. In absorption curves, UV-B was most suitable for octyl methoxycinnamate (OMC) and UV-A for butyl methoxy dibenzoylmethane (BMDM). For this reason, <TEX>$Nylonpoly^{TM}$</TEX> UVA/UVB the material of OMC and BMDM coated with Nylon & polyethylene, was used as the organic UV interceptor. Zinc oxide (ZnO) and titanium dioxide (<TEX>$TiO_2$</TEX>) was used as inorganic UV interceptors. The appropriate mixture ratio of ZnO and <TEX>$TiO_2$</TEX> was 6 to 4:6% of ZnO, 4% of <TEX>$TiO_2$</TEX> and 5% of <TEX>$Nylonpoly^{TM}$</TEX> UVA/UVB were all combined and added to our sunscreen cream. The SPF value of in-vitro was 38.9. In practical application, each sun protection factor (SPF) duration of oil-in-water (O/W) emulsion and water-in-silicone (W/S) emulsion containing sunscreen cream of the same content showed that W/S type of sunscreen cream was 5 times as durable as the other. Therefore, this product is fit for use in swimming, climbing or skiing. This research is to minimize skin trouble caused by UV interceptors and to make one with proper softness, skin safety and UV intercepting efficiency.

keywords
sun protection factor(SPF), cytotoxicity, oil-in-water, water-in-oil, titanium dioxide, zinc oxide, <TEX>$Nylonpoly^{TM}$</TEX> UVA/UVB, Skin irritation


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