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Separation of Functionalized Heterocyclic Compounds by High Performance Liquid Chromatography (II)
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1998, v.11 no.4, pp.292-296
Cho,
Y. J., Lee,
Y. C., Lee,
K., &
Park,
K.
(1998). Separation of Functionalized Heterocyclic Compounds by High Performance Liquid Chromatography (II). Analytical Science and Technology, 11(4), 292-296.
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Abstract
Normal phase or reversed phase liquid chromatographic separation of isoquinoline of heterocyclic compounds and structural isomers of external substituents, <TEX>$COOCH_3$</TEX>, CN and <TEX>$CH_3$</TEX> has been carried out by using several different columns and various mobile phases. From this results, the order of elution of heterocyclic compounds appears to depend on the solvent effect with kinds of mobile phases. Retention mechanism of normal phase system for 2-methylindoline, 2-methylindole, benzoxazole and benzothiazole was also studied depending on adsorption strength between solute and stationary phase of column. However, retention factors of reversed phase system were found on hydrophobic interaction with solvophobic effect.
- keywords
- mobile phases, heterocyclic compound, reversed phase, hydrophobic interaction
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