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Determination of Ginseng Saponins by Reversed-Phase High Performance Liquid Chromatography
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1998, v.11 no.6, pp.436-439
Jeong,
S., Kim,
C., Lee,
N., Choi,
K., Lee,
Y., &
Kim,
I.
(1998). Determination of Ginseng Saponins by Reversed-Phase High Performance Liquid Chromatography. Analytical Science and Technology, 11(6), 436-439.
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Abstract
Ginseng saponins were analysed using reversed-phase high performance liquid chromatography with several columns. The optimum conditions were as following : reverse phase column; Novapak <TEX>$C_{18}$</TEX> ODS column (<TEX>$3.9mm{\times}150mm$</TEX>, <TEX>$5{\mu}m$</TEX>), acetonitrile/water binary mobile phase gradient controller system, solvent flow rate; 1.5 mL/min, and UV (203 nm) detector. The complete separation of ginsenoside <TEX>$Rb_1$</TEX>, <TEX>$Rb_2$</TEX>, Rc, Rd, Re, Rf and <TEX>$Rg_1$</TEX> was achieved within 50 min. The regression coefficients of the calibration curves for seven ginsenosides were 0.98~0.99.
- keywords
- ginsenoside, saponin, reverse-phase high performance liquid chromatography
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