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- E-ISSN 2288-8985
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Preparation of Standard Phosphatidylcholine Hydroperoxide for Chemiluminescence-HPLC Assay
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1998, v.11 no.6, pp.474-477
Song,
J. H., Choi,
H. Y., &
Park,
D.
K.
(1998). Preparation of Standard Phosphatidylcholine Hydroperoxide for Chemiluminescence-HPLC Assay. Analytical Science and Technology, 11(6), 474-477.
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Abstract
A simple and rapid method for preparation of standard phosphatidylcholine hydroperoxide (PCOOH) in chemiluminescence-HPLC assay (CL-HPLC) was developed using the rose bengal dependent spectrofluorometric oxidation. The concentration of obtained PCOOH was determined by FOX (ferrous oxidation-xylenol orange) assay and then subjected to a CL-HPLC system.
- keywords
- chemiluminescence-HPLC, phosphatidylcholine hydroperoxide, oxidation, ferrous oxidationxylenol orange
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