- P-ISSN 1225-0163
- E-ISSN 2288-8985
- Log In/Sign Up
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Mutation Analysis in β<sub>2</sub>-Adrenergic Receptor Gene by Denaturing High Performance Liquid Chromatography (DHPLC)
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2002, v.15 no.3, pp.190-195
Park,
S., Oh,
C., Kim,
J., &
Jang,
W.
(2002). Mutation Analysis in β<sub>2</sub>-Adrenergic Receptor Gene by Denaturing High Performance Liquid Chromatography (DHPLC). Analytical Science and Technology, 15(3), 190-195.
- Downloaded
- Viewed
Abstract
We analysed mutation of <TEX>${\beta}_2$</TEX>-adrenergic receptor gene that controls bronchial asthma by denaturing high performance liquid chromatography (DHPLC) according to ion-pair reversed-phase high performance liquid chromatography (IP-RP-HPLC). We extracted genomic DNA from 50 asthma patients, amplified DNA using PCR, and analysed PCR product by DHPLC. As a result, we obtained that mutation frequency was 15 (30%) among 50 cases. Consequently DHPLC mutation detection was confirmed that the result of direct sequencing was coincide exactly.
- keywords
- DHPLC, IP-RP-HPLC, PCR, mutation
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations
Other articles from this issue
- Analysis of Cyclofenil and its Metabolites in Human Urine using GC/MS
- Mutation Analysis in β2-Adrenergic Receptor Gene by Denaturing High Performance Liquid Chromatography (DHPLC)
- Comparison of isoButoxycarbonyl derivatives, tert.-butyldimethylsilyl derivatives, with US EPA Method in the sensitivity of Alkylphenols, Chlorophenols, and Bisphenol A Potential field-screening applications of GC/MS-SIM
- Adsorptive Catalytic Wave of Chromium-Cupferron Complex
- Analysis of derivatized MX by Gas Chromatography/Mass Spectrometry
- Wettability and Aging Effect of Polystyrene Film Treated by PSII according to the Molecular Weight
- The Studies of Conductive and Non-Conductive Multi-Layer Depth Analysis by Radio Frequency Gas-Jet Boosted Glow Discharge Atomic Emission Spectrometry
- Determination of Diazepam in Intact Diazepam Tablets Using Near Infrared Spectroscopy
- Liquid chromatographic determination of α-, β-, γ-, and δ-tocopherol in sesame oils of different origin
- Simultaneous Removal of NOx/SOx by Catalyst-loaded Cordierite Porous Filter
- A Study on the Characteristics of VOC Removal by Cordierite Filter Loaded with Catalyst
- Residue and Risk Assessment of Polychlorinated dibenzo-p-dioxin/dibenzofurans in the Korean Population
- The Distributions of PCBs in Inchon Coastal Sediments
- Method for the Analysis of Polybrominated Biphenyls(PBBs) in Environmental Samples
- Synthesis and Structural Analysis of the Diaquabis(ethylenediamine)nickel(II) Bis(p-toluenesulfonate) Monohydrate
- Mutation Analysis in β<sub>2</sub>-Adrenergic Receptor Gene by Denaturing High Performance Liquid Chromatography (DHPLC)
- Liquid chromatographic determination of α-, β-, γ-, and δ-tocopherol in sesame oils of different origin
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.