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Mutation Analysis in β<sub>2</sub>-Adrenergic Receptor Gene by Denaturing High Performance Liquid Chromatography (DHPLC)
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2002, v.15 no.3, pp.190-195
Park,
S., Oh,
C., Kim,
J., &
Jang,
W.
(2002). Mutation Analysis in β<sub>2</sub>-Adrenergic Receptor Gene by Denaturing High Performance Liquid Chromatography (DHPLC). Analytical Science and Technology, 15(3), 190-195.
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Abstract
We analysed mutation of <TEX>${\beta}_2$</TEX>-adrenergic receptor gene that controls bronchial asthma by denaturing high performance liquid chromatography (DHPLC) according to ion-pair reversed-phase high performance liquid chromatography (IP-RP-HPLC). We extracted genomic DNA from 50 asthma patients, amplified DNA using PCR, and analysed PCR product by DHPLC. As a result, we obtained that mutation frequency was 15 (30%) among 50 cases. Consequently DHPLC mutation detection was confirmed that the result of direct sequencing was coincide exactly.
- keywords
- DHPLC, IP-RP-HPLC, PCR, mutation
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