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- E-ISSN 2288-8985
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A Study on the potimal HPLC condition for peptides complex analysis using mass spectrometry
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2003, v.16 no.1, pp.78-81
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(2003). A Study on the potimal HPLC condition for peptides complex analysis using mass spectrometry. Analytical Science and Technology, 16(1), 78-81.
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Abstract
Peptides separation in high performance liquid chromatography (HPLC) is very important for the analysis of total proteins using mass spectrometry rather than two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). In this study, we investigated the optimal HPLC condition of peptides for the use of mass fingerprinting. As a result of pursuing a combination of solvent additives for HPLC, water and acetonitile containing both 0.1% trifluoroacetic acid and 0.1% acetic acid respectively showed the most efficient resolution and sensitivity.
- keywords
- liquid chromatography, mass spectrometry, peptides, proteomics
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