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  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

A Study on the potimal HPLC condition for peptides complex analysis using mass spectrometry

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2003, v.16 no.1, pp.78-81


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Abstract

Peptides separation in high performance liquid chromatography (HPLC) is very important for the analysis of total proteins using mass spectrometry rather than two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). In this study, we investigated the optimal HPLC condition of peptides for the use of mass fingerprinting. As a result of pursuing a combination of solvent additives for HPLC, water and acetonitile containing both 0.1% trifluoroacetic acid and 0.1% acetic acid respectively showed the most efficient resolution and sensitivity.

keywords
liquid chromatography, mass spectrometry, peptides, proteomics


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