- P-ISSN 1225-0163
- E-ISSN 2288-8985
- Log In/Sign Up
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Amperometric Determination of Anthracycline Antibiotics with the Mercury Film Thin Layer Flow Cell
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2004, v.17 no.6, pp.470-475
&
(2004). Amperometric Determination of Anthracycline Antibiotics with the Mercury Film Thin Layer Flow Cell. Analytical Science and Technology, 17(6), 470-475.
- Downloaded
- Viewed
Abstract
The mercury film thin layer flow cell (MFTLFC) which yielded the highest sensitivity for the electrochemical reduction of doxorubicin was constructed by coating the glassy carbon working electrode (GCE; A=0.208 cm2) with 5 μL of HgO coating solution (0.5% HgO + 0.25% polystyrene /cyclohexanone) and subsequently followed by applying a potential of -0.40 V for 300 sec in the flow stream of an acetate buffer of pH 4.5. The voltammogram of doxorubicin reached the diffusion current plateau at -0.53 V vs. a Ag/AgCl (3 M NaCl) in the MFTLFC. The diffusion current (Id) of doxorubicin at the MFTLFC was 1.7 times greater than the Id obtained at the TLFC employing a bare glassy carbon working electrode. When the peak areas (electric charge) were plotted vs. concentrations of standard anthracyclines, the calibration factors of doxorubicin and daunorubicin were 1.12 108 μC/M (coefficient of determination; R2: 0.969) and 0.98 108 μC/M (R2: 0.999), respectively in the concentration range between 1.0 × 10-8 M and 1.0 × 10-6 M.
- keywords
- mercury film thin layer flow cell, doxorubicin, daunorubicin, flow injection analysis
Reference
1
(2000) 항생물질의약품기준, 약업신문사
2
(2000) U. S. Pharmacopeia & National Formulary, National Publishing
3
(2000) British Pharmacopoeia, The Stationery Office
4
(1997) European Pharmacopoeia, Council of Europe
Other articles from this issue
- Analytical Methods of Antibiotics in Food and Aqueous Sample
- Method Validation and Uncertainty Estimation for Toluene Measurement in Biological Fluids by HS-GC
- Studies on the Sorption Behavior of Some Metal Ions using XAD-16-Chromotropic Acid Chelating Resin
- Electrochemical behavior and Application of Ruthenium-Cupferron Complex
- Amperometric Determination of Anthracycline Antibiotics with the Mercury Film Thin Layer Flow Cell
- Analysis of Germanium in the Ore Samples by UV-Visible Spectroscopy
- Solvent Extraction of Trace Amount of Ni(II) in Sea Water by using Salen[N,N'-bis(salicylidene)ethylenediamine]
- Consideration of the analytical bias involved in the detection of total volatile organic compounds (TVOC)
- Evaluation of Adsorbent Sampling Methods for Volatile Organic Compoundsin Indoor and Outdoor Air
- A Study on the Measurement of Activity Concentrations of Pu and Am and Their Isotopic Ratios in the Radioactively Contaminated Soil
- Determining Anti-impotence drug-like compounds in Food
- Determination of ethylene glycol in alkali wastewater by gas chromatography/mass spectrometry
- Preconcentration and Determination of Cobalt(II) in Natural Waters using Silica Gel Loaded with 2-Mercaptobenzothiazole
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.