- P-ISSN 1225-0163
- E-ISSN 2288-8985
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
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Amperometric Determination of Anthracycline Antibiotics with the Mercury Film Thin Layer Flow Cell
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2004, v.17 no.6, pp.470-475
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(2004). Amperometric Determination of Anthracycline Antibiotics with the Mercury Film Thin Layer Flow Cell. Analytical Science and Technology, 17(6), 470-475.
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Abstract
The mercury film thin layer flow cell (MFTLFC) which yielded the highest sensitivity for the electrochemical reduction of doxorubicin was constructed by coating the glassy carbon working electrode (GCE; A=0.208 cm2) with 5 μL of HgO coating solution (0.5% HgO + 0.25% polystyrene /cyclohexanone) and subsequently followed by applying a potential of -0.40 V for 300 sec in the flow stream of an acetate buffer of pH 4.5. The voltammogram of doxorubicin reached the diffusion current plateau at -0.53 V vs. a Ag/AgCl (3 M NaCl) in the MFTLFC. The diffusion current (Id) of doxorubicin at the MFTLFC was 1.7 times greater than the Id obtained at the TLFC employing a bare glassy carbon working electrode. When the peak areas (electric charge) were plotted vs. concentrations of standard anthracyclines, the calibration factors of doxorubicin and daunorubicin were 1.12 108 μC/M (coefficient of determination; R2: 0.969) and 0.98 108 μC/M (R2: 0.999), respectively in the concentration range between 1.0 × 10-8 M and 1.0 × 10-6 M.
- keywords
- mercury film thin layer flow cell, doxorubicin, daunorubicin, flow injection analysis
Reference
1
(2000) 항생물질의약품기준, 약업신문사
2
(2000) U. S. Pharmacopeia & National Formulary, National Publishing
3
(2000) British Pharmacopoeia, The Stationery Office
4
(1997) European Pharmacopoeia, Council of Europe
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