- P-ISSN 1225-0163
- E-ISSN 2288-8985
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Analysis of coenzyme Q10 in human plasma by high performance liquid chromatography
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2009, v.22 no.6, pp.514-518
&
(2009). Analysis of coenzyme Q10 in human plasma by high performance liquid chromatography. Analytical Science and Technology, 22(6), 514-518.
- Downloaded
- Viewed
Abstract
Coenzyme Q10(CoQ10), a vitamin E-like substance, represents a components of the complex antioxidant system of the human organism. CoQ10 levels in human plasma were determined by high performance liquid chromatography (HPLC) with UV detection. It was dissociated from lipoproteins by methanol and extracted into n-hexane with liquid-liquid extraction procedure, after centrifugation, the supernatant was dried under nitrogen gas stream. The residue was dissolved in the absolute ethanol. Determination of CoQ10 was performed on a C18 reversed-phase analytical column with ultraviolet detection at 275 nm and the mobile phase containing 15% (v/v) ethanol in methanol at a flow rate of 1.7 mL/min. The low limit of quantitation was 0.02 mg/L (S/N=10), the linearity between the concentration and peak height is from 0.1 to 2.0 mg/L. Twenty-four randomly selected plasma samples from apparently healthy, 27 to 44 year old individuals (males and females) were analyzed for total CoQ10. The average level in these subjects was 0.62±0.13 mg/L with the range of 0.41- 0.98 mg/L. This method has a specific and a sufficient limit of quantitation (LOQ) for analysis of CoQ10 in human plasma in both a clinical study and research at laboratories
- keywords
- coenzyme <TEX>$Q_{10}$</TEX>, antioxidant, micronutrients
Reference
1
1. L. Ernster, ‘Bioimedical and Clinical Aspects of Coenzyme Q’, Amsterdam, Elsevier/North-Holland, 1, 15(1977).
2
2. W. A. Cramer and D. F. Knaff, ‘Energy Transduction in Biological Membranes’, Springer Verlag, Berlin (1990).
3
3. B. L, Trumpower, ‘Function of Quinones in Energy Conservation Systems’, Academic Press, New York (1982).
4
4. P. Mitchell, ‘The vital protomnotive role of coenzyme Q In : Biomedical and Clinical Aspects of Coenzyme Q’, Amsterdam, 6, 3-10(1991).
5
5. J. Kaikkonen, K. Nyyssonen, J. T. Salonen and J. Scand, Clin Lab. Invest., 59(6), 457-466(1999).
6
6. S. Hodges, N. Hertz, K. Lockwood and R. Lister, Biofactors, 9, 365-370(1999).
7
7. M. Takada, S. Ikenoya, T. Yuzuriha and K. Katayama, Biochim. Biophys. Acta, 679, 308-314(1982).
8
8. A. Zhiri and P. Belichard, J. Liq. Chromatogr, 17, 2633- 2640(1994).
9
9. M. A. Pacanowski, R. F. Frye, O. Enogieru, R. S. Schofield and I. Zineh, J. Clin Lipidology., 2. 289- 297(2008).
10
10. J. Karpinska, B. Mikoluc and P. J. Janina, J. Pharm and Biomedical Analysis., 17, 1345-1350(1998).
11
11. P. H. Tang, M. V. Miles, A. DeGrauw, A. Hershery and A. Pesce., Clin Chem., 47(2), 256-265(2001).
12
12. W. K. Lu, O. Zhang, H. S Lee, T. U. Zhou, H. D Sun, D. W Zhang, L. Zheng, M. Lee and S. M Wong, Biol. Pharm. Bull. 26(1), 52-55(2003).
13
13. K. Aikkarach, T. Takayuki, K. Ratchanee, C. Somsri, K. Takeo and O. Tadashi, J. Clin Biochem Nurt., 40. 194-202(2007).
14
14. J. Kucharska, A. Gvozdjakova, S. Mizera, Z. Braunova, Z. Schreineorva, E. Schramekova, I. Pechan and J. Fabian., Physiol. Res. 47, 399-404(1998).
15
15. P. Kaplan, N. Sebestianova, J. Juriakova and I. Kucera, Physiol, Res. 44, 39-45(1995).
16
16. K. Jonansen, H. Theorell, J. Karlsson, B. Diamant and K. Folkers, Ann. Med, 23, 649-656(1991).
17
17. J. Karlsson, B. Kiamant, H. Theorell and K. Folkers, ‘Skeletal muscle coenzyme Q10 in healthy man and selected patient groups. In: Biomedical and Clinical Aspects of Coenzyme Q’, Elsevier Science Publishers. Amsterdam, 191-204(1991).
Other articles from this issue
- Development of the testing method for newly regulated hazardous compounds in electrical and electronic equipments
- Analysis and exposure assessment of the total PCBs in foods
- Determination of residual flumethrin in honey products by HPLC
- Oxidation behavior on the surface of titanium metal specimens at high temperatures (300~1000℃)
- Analytical method of PCBs-containing solid wastes
- The measurement of dicamba in soil and plants
- A study on the calibration characteristics of organic fatty acids designated as new offensive odorants by cryogenic trapping-thermal desorption technique
- Chip-based isothermal amplification method for EGFR gene mutations in lung cancer
- Distribution of hazardous heavy metals in commercial herbal medicines classified by plant parts used in seoul
- Analysis of coenzyme Q10 in human plasma by high performance liquid chromatography
- Removal of I⁻ and IO₃⁻ from Aqueous Solution
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.