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- E-ISSN 2288-8985
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Bioluminescence immunoassay for neurotransmitter, serotonin using aequorin as a Label
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2010, v.23 no.1, pp.60-67
https://doi.org/10.5806/AST.2010.23.1.060
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(2010). Bioluminescence immunoassay for neurotransmitter, serotonin using aequorin as a Label. Analytical Science and Technology, 23(1), 60-67, https://doi.org/10.5806/AST.2010.23.1.060
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Abstract
A sensitive competitive heterogeneous bioluminescence immunoassay for serotonin was developed using photoprotein, aequorin as a label for the first time with the optimal assay conditions; especially, serotoninavidin conjugate was prepared by Mannich reaction and the synthetic process of serotonin-avidin conjugate was optimized by controlling the initial molar ratios of serotonin, formaldehyde and avidin (1:12,000:25). The developed bioluminescence immunoassay for serotonin showed good sensitivity (LOD of 0.68 ng/mL) with wide area of dynamic range (5.0×10−10 M~5.0×10−7 M). (cf. the range for serotonin in human blood serum is 151±45 ng/mL). In addition, cross-reactivity studies demonstrated that 5-methoxytryptamine showed some cross-reactivity (28.0%), whereas 3-methylindole, melatonin and 5-hydroxylindole-3-acetic acid showed no crossreactivity,and good recoveries were obtained in serum. Thus, this developed method provides a good tool to monitor serotonin in serum.
- keywords
- Bioluminescence immunoassay, Aequorin, Neurotransmitter, Serotonin
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