- P-ISSN 1225-0163
- E-ISSN 2288-8985
- Log In/Sign Up
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Studies on resveratrol and its metabolite in human urine by GC/MS
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2011, v.24 no.2, pp.142-149
https://doi.org/10.5806/AST.2011.24.2.142
&
(2011). Studies on resveratrol and its metabolite in human urine by GC/MS. Analytical Science and Technology, 24(2), 142-149, https://doi.org/10.5806/AST.2011.24.2.142
- Downloaded
- Viewed
Abstract
This study was conducted to define metabolite of the resveratrol by gas chromatography- time-offlight mass spectrometric detection. From these results, we suppose that the structure of metabolite is the result of reduction of double-bond attached by two-phenyl groups. Also, validity of method for determining metabolite of resveratrol and endogenous steroids was tested. The recoveries ranged from 96.47 to 114.74%, and intraand inter-day precision ranged 11.40 - 10.87% and 1.10 - 10.93%, accuracy ranged 80.03 - 119.92% and 80.02 -119.56%, respectively. Resveratrol and endogenous steroids had correlation coefficients above or equal to 0.996. The method was successfully validated for the determination of resveratrol and endogenous steroids. Urinary samples from volunteers dosed resveratrol were analyzed to confirm a correlation resveratrol and its metabolite. From these results, the highest level of resveratrol and its metabolite was excreted in 10 - 15 hr more slowly than common drug, and conversion rate of metabolite was higher in woman than that in man. In addition,endogenous steroids were shown same the highest level of 10 - 15 hr. For estrone and estradiol, sensitivity was relatively higher in female than in man. And there were no significant changes of excretion patterns in the other endogenous steroids. Thus, we assumed that activation of resveratrol has impact on woman than man.
- keywords
- resveratrol, metabolite, endogenous steroids, profiling analysis, GC/MS
Reference
1
1. M. Careri, C. Corradini, L. Elviri, I. Nicoletti and I. Zagnoni, J. Agric. Food. Chem., 52, 6868-6874(2004).
2
2. P. Neuza, P. Vanda, J. C. Marques and J. S. Camara, J. Sep. Sci., 31, 2189-2198(2008).
3
3. E. Ignatowicz and W. Baer-Dubowska, Pol. J. Pharmacol., 53, 557-569(2001).
4
4. E. Revilla and J. M. Ryan, J. Chromatogr. A, 881, 461-469(2000).
5
5. Vassilis G. Dourtoglou, Dimitrios P. Makris, Fabienne Bois-Dounasand and Christophoros Zonas, J. Food Compos. Anal., 12, 227-233(1999).
6
6. J. Gruber, S. Y. Tang and B. Halliwell, Ann. NY Acad. Sci., 1100, 530-542(2007).
7
7. T. M. Bass, D. Weinkove, K. Houthoofd, D. Gems and L. Partridge, Mech. Ageing. Dev., 128, 546-552(2007).
8
8. D. R. Valenzano, E. Terzibasi, T. Genade, A. Cattaneo, L. Domenici and A. Cellerino, Curr. Biol., 16, 296-300(2006).
9
9. J. G. Wood, B. Rogina, S. Lavul, K. Howitz, S. L. Helfand, M. Tatar and D. Sinclair, Nature, 430, 686-689 (2004).
10
10. C. R. Pace-Asciak, O. Rounova, S. E. Hahn, E. P. Daimandis and D. M. Goldberg, Clin. Chim. Acta., 246, 163-182(1996).
11
11. M. V. Clement, J. L. Hirpara, S. H. Chawdhury and S. Pervaiz, Blood., 92, 996-1002(1998).
12
12. D. M. Goldberg, J. Yan and G. J. Soleas, Clin. Biochem., 36, 79-87(2003).
13
13. J. J. Cullen, C. Weydert, M. M. Hinkhouse, J. Ritchie, F. E. Domann, D. Spitz and L. W. Oberley, Cancer. Res., 63, 1297-1303(2003).
14
14. E. Wenzel and V. Somoza, Mol. Nutr. Food. Res., 49, 472-481(2005).
15
15. Y. J. Surh, Mutat. Res., 428, 305-327(1999).
16
16. T. L. Zern, R. J. Wood, C. Greene, K. L. West, Y. Liu, D. Aggarwal, N. S. Shachter and M. L. Fernandez, Nutrition, 13, 1911-1917(2005).
17
17. J. J. Anderson and S. C. Garner, Bailliere Clin. Endocrinol. Metabol., 12, 543(1998).
18
18. M. W. Edward, S. D. Bain, M. C. Bailey, M. M. Lantry and G. A. Howard, Bone, 13, 29(1992).
19
19. L. J. Murphy and A. Ghahary, Endocrine Reviews, 11, 443(1990).
20
20. B. D. Gehm, J. M. McAndrews, P. Y. Chien and J. L. Jameson, Proc. Natl. Acad. Sci. USA, 94, 14138(1997).
21
21. R. Lu and Serrero, G., J. Cell. Physiol., 179, 297(1999).
22
22. M. J. Zhou, X. Y. Chen and D. F. Zhong, J. Chromatogr. B, 854, 219(2007).
23
23. R. Gonález-Barrio, M. L. Nidal-Guevara, F. A. Tomás- Barberán and J. C. Espín, Innovat. Food. Sci. Emerg. Tech., 10, 374-382(2009).
24
24. L. S. Cai, J. A. Koziel, M. Dharmadhikari and J. H. Leeuwen, J. Chromatogr. A, 1216, 281(2009).
25
25. M. T. Ribeiro de Lima, P. Waffo-Teguo, P. L. Teissedre, A. Pujolas, J. Vercauteren, J. C. Cabanis and M. Merillon, J. Agric. Food Chem., 47, 2666(1999).
26
26. R. M. Lamuela-Raventos and A. L. Waterhouse, J. Agric. Food. Chem., 41, 521(1993).
Other articles from this issue
- Preparation and analysis of nickel-coated alumina by sonochemistry
- The analysis of ethylene glycol and metabolites in biological specimens
- Electrospray ionization tandem mass fragmentation pattern of camostat and its degradation product, 4-(4-guanidinobenzoyloxy)phenylacetic acid
- A identification of sprayed fire-resistive materials by near-infrared spectroscopy
- Adsorption of MX (3-chloro-4 (dichloromethyl)-5-hydroxy-2-(5H)-furanone) on amphiphilic mesoporous silica in aqueous solution
- Synthesis and property analysis of hydropolysilanes for amorphous and polycrystalline silicon
- Electrochemical kinetic analysis of the carbon paste enzyme electrode bound with butyl rubber
- Determination of benzene, toluene, ethylbenzene and o-xylene in bottled waters by headspace solid-phase microextraction and gas chromatography/mass spectrometry
- Monitoring of preservatives in herbal liquid preparations
- A study of matrix metalloproteinase-9 inhibitor in Hovenia dulcis Thunberg
- Studies on resveratrol and its metabolite in human urine by GC/MS
- Analytical method of aflatoxins in edible oil and infant-children foods
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.