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- E-ISSN 2288-8985
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Spectrofluorometric determination of caffeine using acridine orange-β-cyclodextrin inclusion complex
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2013, v.26 no.6, pp.353-356
https://doi.org/10.5806/AST.2013.26.6.353
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(2013). Spectrofluorometric determination of caffeine using acridine orange-β-cyclodextrin inclusion complex. Analytical Science and Technology, 26(6), 353-356, https://doi.org/10.5806/AST.2013.26.6.353
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Abstract
A method on the determination of caffeine in beverage with acridiene orange-β-cyclodextrin (CD)inclusion complex was developed. The conditions such as pH of the sample solution and concentration of acridine orange and β-CD were optimized to 12.0(±0.5), 1.9 × 10−6 M and 1.25 × 10−3 M, respectively. Under these optimum conditions, the calibration curve of caffeine was obtained over concentration range of 5 × 10−5~ 1.1 × 10−3 M. The detection limit was 1.0 × 10−5 M. The relative errors(%) in beverage samples were less than 5.0%.
- keywords
- caffeine determination, acridine orange, <TEX>${\beta}$</TEX>-cyclodextrin, spectrofluorometry
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