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Separation and identification of selenoproteins in selenium-enriched yeast
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2013, v.26 no.6, pp.357-363
https://doi.org/10.5806/AST.2013.26.6.357
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(2013). Separation and identification of selenoproteins in selenium-enriched yeast. Analytical Science and Technology, 26(6), 357-363, https://doi.org/10.5806/AST.2013.26.6.357
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Abstract
Selenium-containing proteins were separated from selenium-enriched yeast (SEY) using Trizol® reagent followed by anion exchange (AE) chromatography. This method is simpler and less time consuming than electrophoresis. Five selenium containing proteins were identified by on-line AE HPLC-ICP/MS (high performance liquid chromatography-inductively coupled plasma/mass spectrometry). Each protein was enzymatically hydrolyzed to seleno-amino acids and separated with RP (reverse phase) HPLC for the identification of selenoproteins.
- keywords
- Se-enriched yeast, HPLC-ICP/MS, selenoprotein, Trizol<TEX>$^{(R)}$</TEX> reagent, anion exchange chromatography
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