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  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

  • P-ISSN1225-0163
  • E-ISSN2288-8985
  • SCOPUS, ESCI, KCI

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  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

논문 상세

    셀레늄이 강화된 이스트에서 셀레늄 단백질의 분리 및 확인

    Separation and identification of selenoproteins in selenium-enriched yeast

    분석과학 / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
    2013, v.26 no.6, pp.357-363
    https://doi.org/10.5806/AST.2013.26.6.357
    김경미 (한국교원대학교)
    박용남 (한국교원대학교)
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    Abstract

    Selenium-containing proteins were separated from selenium-enriched yeast (SEY) using Trizol® reagent followed by anion exchange (AE) chromatography. This method is simpler and less time consuming than electrophoresis. Five selenium containing proteins were identified by on-line AE HPLC-ICP/MS (high performance liquid chromatography-inductively coupled plasma/mass spectrometry). Each protein was enzymatically hydrolyzed to seleno-amino acids and separated with RP (reverse phase) HPLC for the identification of selenoproteins.

    keywords
    Se-enriched yeast, HPLC-ICP/MS, selenoprotein, Trizol<TEX>$^{(R)}$</TEX> reagent, anion exchange chromatography


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