- P-ISSN 1225-0163
- E-ISSN 2288-8985
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
논문 상세
Home > 논문 상세
셀레늄이 강화된 이스트에서 셀레늄 단백질의 분리 및 확인
Separation and identification of selenoproteins in selenium-enriched yeast
분석과학 / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2013, v.26 no.6, pp.357-363
https://doi.org/10.5806/AST.2013.26.6.357
(한국교원대학교)
(한국교원대학교)
(한국교원대학교)
김경미,
&
박용남.
(2013). 셀레늄이 강화된 이스트에서 셀레늄 단백질의 분리 및 확인. , 26(6), 357-363, https://doi.org/10.5806/AST.2013.26.6.357
- 195다운로드 수
- 500조회수
Abstract
Selenium-containing proteins were separated from selenium-enriched yeast (SEY) using Trizol® reagent followed by anion exchange (AE) chromatography. This method is simpler and less time consuming than electrophoresis. Five selenium containing proteins were identified by on-line AE HPLC-ICP/MS (high performance liquid chromatography-inductively coupled plasma/mass spectrometry). Each protein was enzymatically hydrolyzed to seleno-amino acids and separated with RP (reverse phase) HPLC for the identification of selenoproteins.
- keywords
- Se-enriched yeast, HPLC-ICP/MS, selenoprotein, Trizol<TEX>$^{(R)}$</TEX> reagent, anion exchange chromatography
참고문헌
1
C. Reilly, ‘Selenium in Food and Health’, 2nd ed., Springer, London, 2006.
2
U. Tinggi, Toxicol. Lett., 137, 103-109 (2003).
3
V. Ermakov, L. Jovanovic, J. of Geochem. Explor., 107, 200-205 (2010).
4
H. Qin, J. Zhu and H. Su, Chemosphere, 86, 626-633 (2012).
5
M. Bajaj, E. Eiche, T. Neumann, J. Winter and C. Gallert, J. Hazard. Mater., 189, 640-646 (2011).
6
M. Panigati, L. Falciola, P. Mussini, G. Beretta and R. M. Facino, Food Chemistry, 105, 1090-1098 (2007).
7
M. Germ, V. Stibilj, S. Kreft, A. Gaberšk, F. Pajk and I. Kref, Food Chemistry, 117, 204-206 (2009).
8
C. Lin, L. Wang and K. Shen, J. Clin. Lab. Anal., 23, 192-195 (2009).
9
G .P. Walker, F. R. Dunshea, J. W. Heard and C. R. Stockdale, J. of Dairy Sci., 93, 10, 4644-4650 (2010).
10
K. T. Suzuki, Y. Tsuji, Y. Ohta and N. Suzuki, Toxicol. and Appl. Pharm., 227, 76-83 (2008).
11
Y. Lu, A. Rumler, K. A. Francesconi and S. A. Pergantis, Anal. Chim. Acta, 731, 49-59 (2012).
12
C. Thiry, A. Ruttens, L. D. Temmerman, Y. Schneider and Luc Pussemier, Food Chemistry, 130, 767-784 (2012).
13
K. Pyrzynska, Food Chemistry, 114, 1183-1191 (2009).
14
O. Prince, O. Amoako, P. C. Uden and J. F. Tyson, Anal. Chim. Acta, 652, 315-323 (2009).
15
G. Ballihaut, C. Pe´cheyran, S. Mounicou, H. Preudhomme, R. Grimaud, and R. Lobinski, Trends in Anal. Chem., 26, 183-190 (2007).
16
Z. Pedrero, J. R. Encinar, Y. Madrid and C. Cámara, J. Chromatogr. A, 1139, 247-253 (2007).
17
C. F. Harrington, R. Clough, L. Rovinski, S. J. Hill and J. F. Tyson, J. Anal. At. Spectrom., 26, 1561-1567 (2011).
18
G. Ballihaut, L. X. Kilpatrick, E. L. Kilpatrick and W. Davis, J. Anal. At. Spectrom., 26, 383-394 (2011).
19
L. C. Clark, G. F. Combs, B. W. Turnbull, E. H. Slate, D. K. Chalker, J. Chow, L. S. Davis, R. A. Glover, G. F. Graham, E. G. Gross, A. Krongrad, J. L. Lesher, H. K. Park, B. B. Sanders, C. L. Smith and J. R. Taylor, J. Am. Med. Assoc., 276, 1957-1963 (1996).
20
S. McSheehy, W. Yang, F. Pannier, J. Szpunar, R. Lobinski, J. Auger and M. Potin-Gautier, Anal. Chim. Acta, 421, 147-153 (2000).
21
L. Tastet, D. Schaumloffel, B. Bouyssiere and R. Lobinski, Talanta, 75, 1140-1145 (2008).
22
S. McSheehy, F. Pannier, J. Szpunar, M. Potin-Gautier and R. Lobinski, Analyst, 127, 223-229 (2002).
23
H. Cho and Y. Pak, J. Kor. Chem. Soc., 55, 472-477 (2011).
24
A. Ruttens, L. D. Temmerman, Y. Schneider and L. Pussemier, Food Chemistry, 130, 767-784 (2012).
권호 내 다른 논문
- Acridine orange-β-cyclodextrin 내포 착물을 이용한 카페인의 분광형광법 정량
- 셀레늄이 강화된 이스트에서 셀레늄 단백질의 분리 및 확인
- 제주지역 양돈시설에서 발생하는 악취물질의계절별 농도 및 배출 특성
- 이플루오린화 암모늄 시료분해 및 ICP/MS에 의한암석 및 퇴적물 중 게르마늄 분석
- 테들라 백에서의 알릴클로라이드와 이황화탄소 보존성 연구
- 부산 가덕도 장항 유적 출토 인골의 안정동위원소분석을 통해 본 신석기시대의 식생활 양상
- 항균성 펩타이드와 혼합된 인지질 분자의 상 변화에 있어서수화 효과에 대한 고체 핵자기 공명 연구
- 기체질량분석기를 이용한 유정란 부화과정의 호흡량 분석
- more
추천 논문
- Anti-inflammatory copper nanoparticles for wound medication
- Elemental analysis of nail clippings retrieved from farmers using X-ray fluorescence spectrometry
- Validation and application of a headspace-gas chromatography-mass spectrometric method for determination of toluene and 2-butanol in urine
- Estimation of uncertainty in quantifying urinary lithium via the standard addition method using ion chromatography
- Photostability considerations in preclinical studies: Mini-Review
- Analysis of respiration gas of a fertile chicken egg during incubation by gas mass spectrometer
- A solid-state NMR study on the hydration effect on the lipid phase change in the presence of an antimicrobial peptide
- Spectrofluorometric determination of caffeine using acridine orange-β-cyclodextrin inclusion complex
- Seasonal concentrations and emission characteristics of odorous compounds produced from swine facilities in Jeju Island
- Separation and identification of selenoproteins in selenium-enriched yeast
학술지 홈페이지 이용 환경은 크롬 브라우저 사용을 권장합니다.