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Development and validation of an analytical method for fungicide fenpyrazamine determination in agricultural products by HPLC-UVD
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2014, v.27 no.3, pp.172-180
https://doi.org/10.5806/AST.2014.27.3.172
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(2014). Development and validation of an analytical method for fungicide fenpyrazamine determination in agricultural products by HPLC-UVD. Analytical Science and Technology, 27(3), 172-180, https://doi.org/10.5806/AST.2014.27.3.172
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Abstract
Fenpyrazamine which is a pyrazole fungicide class for controlling gray mold, sclerotinia rot, andMonilinia in grapevines, stone fruit trees, and vegetables has been registered in republic of Korea in 2013 andthe maximum residue limits of fenpyrazamine is set to grape, peach, and mandarin as 5.0, 2.0, and 2.0 mg/kg, respectively. Very reliable and sensitive analytical method for determination of fenpyrazamine residues isrequired for ensuring the food safety in agricultural products. Fenpyrazamine residues in samples were extractedwith acetonitrile, partitioned with dichloromethane, and then purified with silica-SPE cartridge and eluted withhexane and acetone mixture. The purified samples were determined by HPLC-UVD and confirmed with LCMSand quantified using external standard method. Linear range of fenpyrazamine was between 0.1~5.0 μg/mLwith the correlation coefficient (r) 0.999. The average recovery ranged from 71.8 to 102.7% at the spiked levelof 0.05, 0.5, and 5.0 mg/kg, while the relative standard deviation was between 0.1 and 7.3%. In addition, limitof detection and limit of quantitation were 0.01 and 0.05 mg/L, respectively. The results revealed that thedeveloped and validated analytical method is possible for fenpyrazamine determination in agricultural productsamples and will be used as an official analytical method.
- keywords
- fenpyrazamine, pyrazole fungicide, HPLC-UVD, LC-MS
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