- P-ISSN 1225-0163
- E-ISSN 2288-8985
- Log In/Sign Up
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Development and validation of an analytical method for fungicide fenpyrazamine determination in agricultural products by HPLC-UVD
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2014, v.27 no.3, pp.172-180
https://doi.org/10.5806/AST.2014.27.3.172
&
(2014). Development and validation of an analytical method for fungicide fenpyrazamine determination in agricultural products by HPLC-UVD. Analytical Science and Technology, 27(3), 172-180, https://doi.org/10.5806/AST.2014.27.3.172
- Downloaded
- Viewed
Abstract
Fenpyrazamine which is a pyrazole fungicide class for controlling gray mold, sclerotinia rot, andMonilinia in grapevines, stone fruit trees, and vegetables has been registered in republic of Korea in 2013 andthe maximum residue limits of fenpyrazamine is set to grape, peach, and mandarin as 5.0, 2.0, and 2.0 mg/kg, respectively. Very reliable and sensitive analytical method for determination of fenpyrazamine residues isrequired for ensuring the food safety in agricultural products. Fenpyrazamine residues in samples were extractedwith acetonitrile, partitioned with dichloromethane, and then purified with silica-SPE cartridge and eluted withhexane and acetone mixture. The purified samples were determined by HPLC-UVD and confirmed with LCMSand quantified using external standard method. Linear range of fenpyrazamine was between 0.1~5.0 μg/mLwith the correlation coefficient (r) 0.999. The average recovery ranged from 71.8 to 102.7% at the spiked levelof 0.05, 0.5, and 5.0 mg/kg, while the relative standard deviation was between 0.1 and 7.3%. In addition, limitof detection and limit of quantitation were 0.01 and 0.05 mg/L, respectively. The results revealed that thedeveloped and validated analytical method is possible for fenpyrazamine determination in agricultural productsamples and will be used as an official analytical method.
- keywords
- fenpyrazamine, pyrazole fungicide, HPLC-UVD, LC-MS
Reference
1
1. Byeong-sub, K. Fungicide resistance and physiological and ecological diversity of Botrytis cinerea. Ph. D. Dissertation, The Graduate School of Seoul National University, Seoul, Korea (1996).
2
2. EFSA, ‘Conclusion on the peer review of the pesticide risk assessment of the active substance fenpyrazamine’, EFSA Journal, 10(1), 2496-2557 (2012).
3
3. S. S. Hong, M. H. Jeong, A. S. Yoo, C. R. Park, K. H. Park, J. E. Park and K. B. Chang, 2012 Annual Conference of Korean Society of Pesticide Science, 175 (2012).
4
4. S. Tanaka, R. Ishikawa, P. Armengaud and Y. Senechal, 10th International Conference on Diseases of Plants, Tours, France, 680-691 (2012).
5
5. EFSA, ‘Reasoned opinion on the modification of .the existing MRLs for fenpyrazamine in almonds, grapes, apricots, peaches and strawberries’, EFSA Journal, 10(11), 2989-3020 (2012).
6
6. EPA, ‘Fenpyrazamine; Pesticide Tolerances’, 78(44), 14461-14465 (2013).
7
7. National Academy of Agricultural Science, ‘Risk assessment and ADI establishment of fenpyrazamine’ (2012).
8
8. Ministry of Food and Drug Safety, http://fse.foodnara. go.kr/residue/pesticides/pesticides_info.jsp, Accessed 2 July 2013.
9
9. Y. D. Lee, ‘Practical book of Korea Food Code pesticide residue analysis method’, 3rd Ed. KFDA, Osong, 2012.
10
10. CAC, ‘Guidelines on good laboratory practice in residue analysis’, CAC/GL 40, 2nd Ed., Codex, Rome, 2003.
11
11. E. C., ‘Method validation and quality control procedures for pesticide residues analysis in food and feed’, SANCO/10684/2009, E.C., Bruxelle, 2009.
12
12. C. H. Kwon, M. H. Chang, M. H. Im, D. I. Choi, S. C. Jung, J. Y. Lee, Y. D. Yu, J. O. Lee and M. K. Hong, J. Anal. Sci. Technol., 21(6), 518-525 (2008).
13
13. S. J. Lee, Y. H. Kim, L. W. Song, Y. S. Hwang, J. D. Lim, E. H. Sohn, M. H. Im, J. A. Do, J. H. Oh, K. S. Kwon KS, J. K. Lee, Y. D. Lee and M. G. Choung, Korean J. Pest. Sci., 15(3), 254-268 (2011).
Other articles from this issue
- Aptamer-based optical switch for biosensors
- Study of improving precision and accuracy by using an internal standard in post column isotope dilution method for HPLC-ICP/MS
- Effect of forensic short wavelength UV on DNA
- A study on the quantitation of asbestos by the visual estimation and point counting method
- Development of alternative assay method without hazardous reagent, carbon tetrachloride, for cefroxadine capsules in Korean Pharmaceutical Codex
- Analytical method for combustible waste contaminated by the HF leakage from industrial process
- Development and validation of an analytical method for fungicide fenpyrazamine determination in agricultural products by HPLC-UVD
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.