- P-ISSN 1225-0163
- E-ISSN 2288-8985
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Performance of MiniPCRTM mini8, a portable thermal cycler
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2016, v.29 no.2, pp.79-84
https://doi.org/10.5806/AST.2016.29.2.79
&
(2016). Performance of MiniPCRTM mini8, a portable thermal cycler. Analytical Science and Technology, 29(2), 79-84, https://doi.org/10.5806/AST.2016.29.2.79
- Downloaded
- Viewed
Abstract
A small and inexpensive thermal cycler (PCR machine), known as the MiniPCRTM Mini8 Thermal Cycler (Amplyus, Cambridge, MA, USA), was developed. In this study, the performance of this PCR machine was compared with the GeneAmp® PCR system 9700 (Applied Biosystems) using four autosomal short tandem repeat (STR) kits, a Y-chromosome STR kit, and a mitochondrial DNA HV1/HV2 sequence analysis. The sensitivity and stochastic effects of the STR multiplex kits and the quality of the DNA sequence analysis were similar between the two PCR machines. The MiniPCRTM Mini8 Thermal Cycler could be used for analyses at forensic DNA laboratories and crime scenes. The cost of the PCR is so economical that school laboratories and individuals could use the machines.
- keywords
- MiniPCR<sup>TM</sup> mini8 Thermal Cycler, GeneAmp®, PCR system 9700, performance, STR multiplex kits, mtDNA HV1/HV2
Reference
1
1. Bartlett, J. M. S. and Stirling, D. “A Short History of the Polymerase Chain Reaction”. PCR Protocols. Methods in Molecular Biology, 226 (2nd ed.). pp. 3-6. doi:10.1385/1-59259-384-4:3. ISBN 1-59259-384-4 (2003).
2
2. Newton, C. R. Graham, A., Heptinstall, L. E., Powell, S. J., Summers, C., Kalsheker, N., Smith, J. C., and Markham, A. F., Nucleic Acids Research, 17(7), 2503-2516 (1989).
3
3. Stemmer, W. P., Crameri, A., Ha, K. D., Brennan, T. M., and Heyneker, H. L., Gene, 164(1), 49-53 (1995).
4
4. Hayden, M. J., Nguyen, T. M., Waterman, A., and Chalmers, K. J., BMC Genomics, 9, 80, doi: 10.1186/1471-2164-9-80 (2008).
5
5. Bartlett, J. M. J. Forensic Sci., 51, 253-265 (2006).
6
6. Hammond, H. A., Jin, L., Zhong, Y., Caskey, C. T., and Chackraborty, R., Am J. Hum. Genet., 55, 175-189 (1994).
7
7. Tautz, D., Nucleic Acids. Res., 17, 6463-6471 (1989).
8
8. Lee, E. J., Choung, C. M., Moon, S. H., Chung, J. Y., Jin, G. N., Kim, M. I., Lee, J. Y., Kim, S. H., and Lee, Y. H., Korean J. Forensic Sci., 15(1), 87-90 (2014).
Other articles from this issue
- Residue levels of phthalic acid esters (PAEs) and diethylhexyl adipate (DEHA) in various industrial wastewaters
- Study of the determination of 226Ra in soil using liquid scintillation counter
- A case study on the fire victim in the vehicle by GC/MS through derivatization of cyanide with pentafluorobenzyl bromide (PFBBr)
- Performance of MiniPCRTM mini8, a portable thermal cycler
- Risk of DNA contamination through fingerprint brush, during the dusting of living persons and deceased
- Development of analytical method for determination of spinetoram residues in livestock using LC-MS/MS
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.