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- E-ISSN 2288-8985
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The stability comparison of purified bee venom and bee venom melittin in aqueous solution
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2016, v.29 no.4, pp.194-201
https://doi.org/10.5806/AST.2016.29.4.194
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(2016). The stability comparison of purified bee venom and bee venom melittin in aqueous solution. Analytical Science and Technology, 29(4), 194-201, https://doi.org/10.5806/AST.2016.29.4.194
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Abstract
The stability comparison tests on purified bee venom (PBV) and bee venom melittin (BVM) in different conditions of temperature, solvent, and concentration were studied. High purity BVM (98.2 %) was separated from PBV by prep-HPLC (column, C4) and used to stability tests in aqueous phase. The stability of the PBV has been increased in the saline solution, while BVM was reduced. In distilled water, the stability of PBV has been reduced, while BVM showed an increasing result. As a result, the appropriate conditions for maintaining the long term stability of BVM were found to be the low temperature (4 oC), distilled water, and concentration (1.0 mg/mL).
- keywords
- Stability, Quantitative analysis, Prep-HPLC, Purified bee venom (PBV), Bee venom melittin (BVM)
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