- P-ISSN 1225-0163
- E-ISSN 2288-8985
- Apply for Authority
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
The stability comparison of purified bee venom and bee venom melittin in aqueous solution
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2016, v.29 no.4, pp.194-201
https://doi.org/10.5806/AST.2016.29.4.194
&
(2016). The stability comparison of purified bee venom and bee venom melittin in aqueous solution. Analytical Science and Technology, 29(4), 194-201, https://doi.org/10.5806/AST.2016.29.4.194
- Downloaded
- Viewed
Abstract
The stability comparison tests on purified bee venom (PBV) and bee venom melittin (BVM) in different conditions of temperature, solvent, and concentration were studied. High purity BVM (98.2 %) was separated from PBV by prep-HPLC (column, C4) and used to stability tests in aqueous phase. The stability of the PBV has been increased in the saline solution, while BVM was reduced. In distilled water, the stability of PBV has been reduced, while BVM showed an increasing result. As a result, the appropriate conditions for maintaining the long term stability of BVM were found to be the low temperature (4 oC), distilled water, and concentration (1.0 mg/mL).
- keywords
- Stability, Quantitative analysis, Prep-HPLC, Purified bee venom (PBV), Bee venom melittin (BVM)
Reference
1
1. S. M. Han, K. G. Lee, J. H. Yeo, H. Y. Kwon, H. J. Baek and K. K. Park, J. Med. Plants. Res., 4(6), 459-464 (2010).
2
2. M. H. Kim, Education Plan of Korea, 67-141 (1992).
3
3. S. T. Kim, J. Y. Hwang, M. S. Sung, S. Y. Je, D. R. Bae, S. M. Han and S. H. Lee, Korean J. Vet. Serv., 29(1), 19-26 (2006).
4
4. Z. J. Kokot and J. Matysiak, Chromatographia, 69, 1401-1405 (2009).
5
5. R. V. Ameratunga, R. Hawkins, R. Prestidge and J. Marbrook, Pathology, 27(2), 157-160 (1995).
6
6. H. R. Chmielewska and T. Szezesna, J. Apic. Sci., 48, 103-109 (2004).
7
7. H. K. Ko and K. R. Kwon, Publication of KyungHee University, 106-107 (2003).
8
8. T. C. Terwilliger and D. Eisenberg, J. Biological Chem., 257(11), 6016-6022 (1982).
9
9. J. D. Dunn and J. J. Killion, Acta Endocrinol., 119(3), 339-344 (1988).
10
10. H. N. Kim and S. S. Nam, Korean Inst. of Herbal Acupunc., 3(1), 65-87 (2000).
11
11. J. S. Choi, J. W. Park and M. S, Oh, Daejeon University Orient. Med. Symp., 15(1), 141-160 (2006).
12
12. W. Neumann, E. Habermann and G. Amend, Naturwissenschaften, 39, 286-287 (1952).
13
13. J. S. Lee, J. Y. Lee, K. R. Kwon and H. C. Lee, J. Pharmacopunct., 9(3), 61-77 (2006).
14
14. J. D. Lee, J. Korea Orient. Med., 21(3), 3-8 (2000).
15
15. M. Cho, S. Han, J. Kim, J. Yeo, I. Hong, S. Woo and K. Lee, J. Seric. Entomol. Sci., 52(1), 6-9 (2014).
16
16. Y. Miura, Eur, Biophys. J., 41, 629-636 (2012).
17
17. P. Venkatesu, M. J. Lee and H. M. Lin, Biochem. Eng. J., 32, 157-170 (2006).
18
18. C. Liao, M. S. Selvan and J. Zhao, etc., J. Phys. Chem. B., 119(33), 10390-10398 (2015).
Other articles from this issue
- Analysis of erectile dysfunction drugs and their analogues in counterfeit drugs and herbal medicines by LC-ESI-MS/MS
- Comparative bioavailability of synthetic vitamin C and Nutra-C (calcium ascorbate) in Korean healthy volunteers
- A comparison study of 76Se, 77Se and 78Se isotope spikes in isotope dilution method for Se
- Simultaneous analysis of β-lactam antibiotics and β-blockers by LC-MS/MS
- The anti-histamine effect of water soluble alkaloids extracted from solanum nigrum L.
- The stability comparison of purified bee venom and bee venom melittin in aqueous solution
- Development of HPLC-UV method for detection and quantification of seven organic acids in animal feed
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.