- P-ISSN 1225-0163
- E-ISSN 2288-8985
- Apply for Authority
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Development of HPLC-UV method for detection and quantification of seven organic acids in animal feed
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2016, v.29 no.4, pp.202-208
https://doi.org/10.5806/AST.2016.29.4.202
&
(2016). Development of HPLC-UV method for detection and quantification of seven organic acids in animal feed. Analytical Science and Technology, 29(4), 202-208, https://doi.org/10.5806/AST.2016.29.4.202
- Downloaded
- Viewed
Abstract
A novel and simple method for detecting seven organic acids (formic acid, malic acid, lactic acid, acetic acid, citric acid, fumaric acid and propionic acid) in animal feed using high performance liquid chromatography equipped with a photo-diode array detector (HPLC/PDA) was developed. The chromatographic peaks of the seven organic acids were successfully identified by comparing their retention times and UV spectra with reference standards. Method validation was performed in terms of linearity, sensitivity, selectivity, accuracy, and precision. The limits of detection (LODs) for the instrument employed in these experiments ranged from 12 to 8,026 μg/kg, and the limits of quantification (LOQs) ranged from 43 to 26,755 μg/kg. Average recoveries of the seven organic acids ranged from 79.3 to 95.2 %. Method replication resulted in intra- day and interday peak area variation of <3.2 %. The developed method was specific and reliable and is therefore suitable for the routine analysis of organic acids in animal feed.
- keywords
- organic acid (formic acid, malic acid, lactic acid, acetic acid, citric acid, fumaric acid, propionic acid), animal feed, hplc, simultaneous
Reference
1
1. D. W. Kim, J. H. Kim, S. K. Kim, G. H. Kang, H. K. Kang, S. J. Lee and S. H. Kim, J. Anim. Sci. Technol., 51, 207-216 (2009).
2
2. J. W. Joo, J. Y. Choi, S. C. Choi, W. T. Cho, B. J. Chae and Y. X. Yang, J. Anim. Sci. Technol., 51, 15-24 (2009).
3
3. K. H. Partanen and Z. Mro, Nutr. Res. Rev., 12, 117-145(1991).
4
4. I. H. Hwang, Feed Industry, 2, 58-65 (2004).
5
5. H. K. Yoon, Y. H. Kim and J. H. Han, Korean J. Vet. Serv., 32(1), 77-82 (2009).
6
6. X. Yin, Jianghua Li, H. D. Shin, Guocheng Pu, Long Liu and Jian Chen, Biotechnol. Adv., 33, 830-841(2015).
7
7. C. H. Lee, I. H. Jo, J. C. Shon and S. H. Lee, Korean J. Org. Agric., 17, 237-251 (2009).
8
8. Sung Su Ill, Ministry for Food, Agriculture, Forestry and Flsheries (MIFAFF), 506028-3 (2009).
9
9. Standard and specification for animal feeds, Ministry of Agriculture. Food and Rural Affairs (MAFRA), 2014-45 (2014.04.29).
10
10. Rodrigo Scherer, Ana Cecilia Poloni Rybka, Cristiano Augusto Ballus, Adriana Dillenburg Meinhart, Jose Teixeira Filho, Helena Teixeira Godoy, Food Chem., 135, 150-154 (2012).
11
11. Hans A. Eyeghe-Bickong, Erik O. Alexandersson1, Liezel M. Gouws, Philip R. Young and Melane A. Vivier, J. Chromatogr. B, 885-886, 43-49 (2012).
12
12. Giuseppe Zeppa, Lorenza Conterno and Vincenzo Gerbi, Agric. Food Chem., 59, 2722-2726 (2001).
13
13. Jorge Bouzas, Carlos A. Kantt, Floud Bodyfelt and J. Antonio, Torres, Food Sci., 56, 276-278 (1991).
14
14. Fabio Chinnici, Umberto Spinabelli, Claudio Riponi and Aureliano Amati, J. Feed Compos. Anal., 18, 121-130 (2005).
15
15. X.-Q. Wang, F.-Y. Wei, Z.-F. Wei, L. Zhang, M. Luo, Y.-H. Zhang, Y.-G. Zu and Y.-J. Fu, Sep. Purif. Technol., 35, 80-87 (2014).
16
16. Ute Roessner, Cornelia Wagner, Joachim Kopka, Richard N. Trethewey and Lothar Willmitzer, Plant J., 23, 131-142 (2000).
17
17. M.-H. Yanga and Y.-M. Choongb, Food Chem., 75, 101-108 (2001).
18
18. ICH International Conference Harmonization, Validation of analytical procedures: test and methodology (2005).
19
19. M. Thompson, S. L. R. Ellison and R. Wood, Int. Union Pure Appl. Chem.(IUPAC), 74, 835-855 (2002).
Other articles from this issue
- Analysis of erectile dysfunction drugs and their analogues in counterfeit drugs and herbal medicines by LC-ESI-MS/MS
- Comparative bioavailability of synthetic vitamin C and Nutra-C (calcium ascorbate) in Korean healthy volunteers
- A comparison study of 76Se, 77Se and 78Se isotope spikes in isotope dilution method for Se
- Simultaneous analysis of β-lactam antibiotics and β-blockers by LC-MS/MS
- The anti-histamine effect of water soluble alkaloids extracted from solanum nigrum L.
- The stability comparison of purified bee venom and bee venom melittin in aqueous solution
- Development of HPLC-UV method for detection and quantification of seven organic acids in animal feed
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.