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- E-ISSN 2288-8985
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Do-It-Yourself (DIY) manufacture of a Nano-LC MALDI spotter robot using 3D printing technology
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2017, v.30 no.4, pp.167-173
https://doi.org/10.5806/AST.2017.30.4.167
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(2017). Do-It-Yourself (DIY) manufacture of a Nano-LC MALDI spotter robot using 3D printing technology. Analytical Science and Technology, 30(4), 167-173, https://doi.org/10.5806/AST.2017.30.4.167
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Abstract
In the era of the forth Industrial Revolution, open source code and open source hardware have gained much attention. In particular, 3D printing technology is expanding into the realms of classical science, technology and our daily lives. Relatedly, in the present study, we demonstrate the manufacture of a nano-LC MALDI spotter robot using 3D printing technology. The parts of the spotter robot were either made using a 3D printer or purchased as 3D printer parts from the 3D printer online market, so that anyone can make the robot without a deep knowledge of engineering or electronics, i.e., DIY (do-it-yourself) product. In the nano-LC MALDI spotter, the nano-LC eluent and MALDI matrix were mixed in a T-union and discharged from the capillary outlet. The eluent and matrix mixture could be spotted onto the movable MALDI plate. The MALDI plate was designed to translate in a two-dimensional space (xy plane), which was enabled by the movements of two stepper motors. In the paper, all computer-aided design (CAD) files for the parts and operation software are provided to help the reader manufacture their own spotter robot.
- keywords
- MALDI, Do-it-yourself, Spotter, Robot, 3D printing, liquid chromatography
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