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  • P-ISSN1225-0163
  • E-ISSN2288-8985
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  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

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    Comparative analysis of yeast cell viability at exponential and stationary growth phases

    분석과학 / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
    2022, v.35 no.4, pp.181-188
    https://doi.org/10.5806/AST.2022.35.4.181
    Yejin An (Department of Plant & Biomaterials Science, College of Natural Sciences, Gyeongsang National University)
    Nayoon Jo (Department of Plant & Biomaterials Science, College of Natural Sciences, Gyeongsang National University)
    Hyeji Kim (Department of Plant & Biomaterials Science, College of Natural Sciences, Gyeongsang National University)
    Dahye Nam (Department of Plant & Biomaterials Science, College of Natural Sciences, Gyeongsang National University)
    Woorim Son (Department of Plant & Biomaterials Science, College of Natural Sciences, Gyeongsang National University)
    Jinkyu Park (Department of Plant & Biomaterials Science, College of Natural Sciences, Gyeongsang National University)
    Yejin, A. , Nayoon, J. , Hyeji, K. , Dahye, N. , Woorim, S. , & Jinkyu, P. (2022). . 분석과학, 35(4), 181-188, https://doi.org/10.5806/AST.2022.35.4.181
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    Abstract

    This paper describes a comparative analysis of yeast cell viability at exponential and stationary growth phases using multiple conventional techniques and statistical tools. Overall, cellular responses to various viability assays were asynchronous. Results of optical density measurement and direct cell counting were asynchronous both at exponential and stationary phases. Proliferative capacity measurement using SP-SDS indicated that cells at the end of the stationary phase were proliferative as much as exponentially growing cells. Metabolic activity assays using two different dyes concluded that the inside of cells at stationary phase is slightly less reducing compared to that of exponentially growing cells, implying that the metabolic activity imperceptibly declined as cells were aged. These results will be helpful to understand the details of yeast cell viability at exponential and stationary growth phases.

    keywords
    yeast, cell viability, exponential phase, stationary phase, proliferative capacity, metabolic activity


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