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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

Inhibition of cell growth and induction of apoptosis by Euonymus alatus (Thunb.) Sieb in human leiomyomal smooth muscle cells

대한한의학회지 / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2004, v.25 no.4, pp.108-120
Yi-Geun Kim (동국대학교)
Ji-Young Han (동국대학교)
Young-Soo Park (동국대학교)
Dong-Il Kim (동국대학교)
Tae-Kyun Lee (Dr.Lee's Traditional Korean Medical Clinic)
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Abstract

Objectives : Euonymus alatus (Thunb.) Sieb (EA) is a traditional Korean herbal medicine, commonly used to treat tumors in Korea and China for centuries. Several earlier studies have indicated that EA exhibits anti-tumor properties, but its mechanism remains to be elucidated. In this study, we evaluated the molecular mechanism of EA in a human uterine leiomyomal smooth muscle cell (ULSMC) line.Methods : This study was evaluated by: (a), morphological changes by using acridine orange/ethidium bromide staining; (b), DNA fragmentation by TdT-mediated dUTP nick end labeling (TUNEL); and (c), sub-G1 cell analysis. Results : This study observed that EA treatment caused apoptotic cell death and depletion of intracellular glutathione (GSH) and that reduction of mitochondrial membrane potential was found to be involved in the initiation of apoptosis by EA. Conclusion : This results show that EA exerted clear cytotoxic effects and strongly inhibited the proliferation of ULSMC.

keywords
Euonymus alatus (Thunb.) Sieb (EA), uterine leiomyomal smooth muscle cells (ULSMC), myometrial smooth muscle cells (MSMC)., Euonymus alatus (Thunb.) Sieb (EA), uterine leiomyomal smooth muscle cells (ULSMC), myometrial smooth muscle cells (MSMC).


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