- P-ISSN 1010-0695
- E-ISSN 2288-3339
Objects : We studied the effect of Bambusae concretio Silicea (BCS) on bone metabolism. Methods : At first, we treated PTH, 1,25(OH)2D3, mononuclear cell conditioned medium (MCM) and IL-1 to osteoblast cells derived from mouse calvarial bone explants in vitro, and then investigated the activities of collagenolysis and bone resorption factors. Results : BCS extracts have no cytotoxicities in concentrations of 1-150 μg/ml. BCS had protective activity against PTH (5 units/ml), MCM (5%, v/v), 1,25(OH)2D3 (20 ng/ml), IL-1α (2 ng/ml) and IL-1β (1 ng/ml)-induced collagenolysis in the mouse calvarial cells. And, pretreatment of BCS for 1 hr significantly reduced the collagenolysis. Furthermore, it was much more expressed at 16 hrs after BCS (50 μg/ml)-pretreatment. And, BCS significantly protected against enhanced collagenolysis induced by IL-1α and IL-1β. Conclusion : BCS extracts inhibited the bone resorption in mouse calvarial bone cell;, thus BCS could be used clinically for bone diseases.
(1981) Parathyroid hormone, calcitonin, and calciferols In Williams, R. H. ed) Textbook of endocrinology., W.B. Saunders Co
(1981) The physiology of bone, Clarendon Press
(1987;2) Synergism between parathyroid hormone and interleukin 1 in stimulating bone resorption in organ culture,
(1994;1224) Rabbit calvarial osteoblasts in culture constitutively synthesize progelatinase-A,
(1992;182) Consitutive expression of c-fos gene inhibits type-I collagen synthesis in transfected osteoblasts,
Processing of precursor Interleukin 1β and inflammatory disease,
(1990;166) The effects of recombinant human interleukin-1β on cellular proliferation and the production of prostaglandin E2 osteocalcin and alkaline phosphatase by osteoblast-like cells derived from human bone,
(1993;28) Changes in crevicular fluid levels of interleukin-1β thromboxane B2 and tumor necrosis factor α in experimental gingivities in humans,
(1994;135) Regulation of the two prostaglandin G/H synthases by parathyroid hormone cortisol and prostaglandin E2 in cultured neonatal mouse calvariae,
(1994;9) Stimulation of prostaglandin E2 production by interleukin-1α and transforming growth factor α in osteoblastic MC3T3-E1 cells,
(1986;138) Stimulation of prostaglandin E2 and bone resorption by recombinant human interleukin-1α in fetal mouse bone,
(1989;125) Effects of interleukin-1 on bone turnover in normal mice,
(1986;453-97) Clinical Application of Oriental Medicinal Herbs, Sungbo Publishing Co
(1992;17) Inhibitory effects of the extract of Bambusae concretio Silicea on monoamine oxidase in aged mice,
(1999) Effects of Bambusae concretio Silicea on amyloid-β-induced neuronal cell toxicity lipid peroxidation and antioxidative enzymes in rat neuronal astrocytes Thesis of Doctor's degree, Dongguk University
(2000) Effects of Bambusae concretio Silicea on mouse calvarial bone resorption Thesis of Master's degree, Dongguk University
(1999) Inhibitory effects og streptozotocin, tumornecrosis factor-α,and interleukin-1β on glucokinase activity in pancreatic islets and gene expression of GLUT2 and glucokinase,
(1996) Interleukin-4 inhibits prostaglandin G/H synthase-2 and cytosolic phospholipase A2 induction in neonatal mouse parietal bone cultures,
(1951;193) Protein measurement with the Folin phenol reagent,
(1969;85) Effect of phosphate calcium and magnesium on bone resorption and hormonal responses in tissue culture,
(1996) Principles of Bone Biology., Academic Press
(1985;134) The four biochemically distinct species of human interleukin 1 all exhibit similar biologic activities,