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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

Effects of Bambusae concretio Silicea on Suppression of Collagenolysis and Bone Resorption in Mouse Calvarial Osteoblasts

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2004, v.25 no.4, pp.15-25
Seong-Choon Lee
Cheol-Ho Yoon
Ji-Cheon Jeong
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Abstract

Objects : We studied the effect of Bambusae concretio Silicea (BCS) on bone metabolism. Methods : At first, we treated PTH, 1,25(OH)2D3, mononuclear cell conditioned medium (MCM) and IL-1 to osteoblast cells derived from mouse calvarial bone explants in vitro, and then investigated the activities of collagenolysis and bone resorption factors. Results : BCS extracts have no cytotoxicities in concentrations of 1-150 μg/ml. BCS had protective activity against PTH (5 units/ml), MCM (5%, v/v), 1,25(OH)2D3 (20 ng/ml), IL-1α (2 ng/ml) and IL-1β (1 ng/ml)-induced collagenolysis in the mouse calvarial cells. And, pretreatment of BCS for 1 hr significantly reduced the collagenolysis. Furthermore, it was much more expressed at 16 hrs after BCS (50 μg/ml)-pretreatment. And, BCS significantly protected against enhanced collagenolysis induced by IL-1α and IL-1β. Conclusion : BCS extracts inhibited the bone resorption in mouse calvarial bone cell;, thus BCS could be used clinically for bone diseases.

keywords
Bambusae concretio Silicea (BCS), collagenolysis, bone resorption, Bambusae concretio Silicea (BCS), collagenolysis, bone resorption


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