Article Detail

Home > Article Detail
  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

Inhibition of cell growth and induction of apoptosis by Euonymus alatus (Thunb.) Sieb in human leiomyomal smooth muscle cells

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2004, v.25 no.4, pp.108-120
Yi-Geun Kim
Ji-Young Han
Young-Soo Park
Dong-Il Kim
Tae-Kyun Lee (Dr.Lee's Traditional Korean Medical Clinic)
  • Downloaded
  • Viewed

Abstract

Objectives : Euonymus alatus (Thunb.) Sieb (EA) is a traditional Korean herbal medicine, commonly used to treat tumors in Korea and China for centuries. Several earlier studies have indicated that EA exhibits anti-tumor properties, but its mechanism remains to be elucidated. In this study, we evaluated the molecular mechanism of EA in a human uterine leiomyomal smooth muscle cell (ULSMC) line.Methods : This study was evaluated by: (a), morphological changes by using acridine orange/ethidium bromide staining; (b), DNA fragmentation by TdT-mediated dUTP nick end labeling (TUNEL); and (c), sub-G1 cell analysis. Results : This study observed that EA treatment caused apoptotic cell death and depletion of intracellular glutathione (GSH) and that reduction of mitochondrial membrane potential was found to be involved in the initiation of apoptosis by EA. Conclusion : This results show that EA exerted clear cytotoxic effects and strongly inhibited the proliferation of ULSMC.

keywords
Euonymus alatus (Thunb.) Sieb (EA), uterine leiomyomal smooth muscle cells (ULSMC), myometrial smooth muscle cells (MSMC)., Euonymus alatus (Thunb.) Sieb (EA), uterine leiomyomal smooth muscle cells (ULSMC), myometrial smooth muscle cells (MSMC).


Reference

1

Kitanaka, S.,, (1996) Cytotoxic cardenolides from woods of Euonymus alata,

2

Medica World Scientific, (1986) Pharmacology and applications of chinese materia,

3

Kim CH, (2003) Effect of buthanol and methanol extracts from Euonymus Alatus on matrix metalloproteinase-9 in cervical epithelial carcinoma cells ,

4

Cha BY, (2002) Inhibitory effect of methanol extract from Euonymus Alatus on matrix metalloproteinase-9,

5

Huang KC, (1993) The pharmacology of Chinese herbs, CRC Press

6

Kitanaka S., , (1996) Cytotoxic cardenolides from woods of Euonymus alata,

7

Kerr JFR, (1994;73) Apoptosis its significance in cancer and cancer therapy,

8

Paschka AG,, (1998) Induction of apoptosis in prostate cancer cell lines by the green tea component(-)-epigallocatechin-3-gallate.,

9

Yanagihara K, (1993;53) Antiproliferative effects of isoflavines on human cancer cell lines established from the gastrointestinal tract,

10

Diomede L, (1994;57) The induction of apoptosis is a common feature of the cytotoxic action of ether-linked glycerophospholipids in human leukemic cells, Principe P

11

Jiang B,, (1995) Induction of apoptosis by enediyne antitumor antibiotic C1027 in HL-60 promyelocytic leukemia cells,

12

Piazza GA,, (1995) Antineoplastic drugs sulindac sulfide and sulfone inhibit cell growth by inducing apoptosis,

13

Smets LA, (1994;5) and response to anti-cancer drugs,

14

Hansen MB, (1989;119) Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill,

15

Shen HM, (1995) Involvement of reactive oxygen species in aflatoxin B1-induced cell injury in cultured rat hepatocytes,

16

Martin SJ, (1995) The end of the(cell)line: methods for the study of apoptosis in vitro,

17

Sherman Y, (1992;119) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation,

18

Shen HM, (81) Sodium selenite-induced oxidative stress and apoptosis in human hepatoma HepG2 cells,

19

Nicoletti L, (1991;139) A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry,

20

Ann, (1976;74) A fluorometric method for determination of oxidized and reduced glutathione in tissues,

21

Sureda FX,, (1997) Mitochondrial membrane potential measurement in rat cerebellar neurons by flow cytometry,

22

Ahmad N, (1997) Green tea constituent epigallocatechin-3-gallate and induction of apoptosis and cell cycle arrest in human carcinoma cells,

23

Hall AG, (1999) The role of glutathione in the regulation of apoptosis,

24

Watson RWG, (1996) Thiol-mediated redox regulation of neutrophil apoptosis,

25

Van den Dobbelsteen DJ, (1996) Rapid and specific efflux of reduced glutathione during apoptosis induced by anti-Fas/APO-1 antibody,

26

Petit PX, (1997) Implication of mitochondria in apoptosis,

27

Kroemer G, (1997) Mitochondrial control of apoptosis,

28

Kluck RM,, (1997) The release of cytochrome c from mitochondria a primary site for Bcl-2 regulation of apoptosis,

29

Susin SA , (1996) Bcl-2 inhibits the mitochondrial release of an apoptogenic protease,

  • Downloaded
  • Viewed
  • 0KCI Citations
  • 0WOS Citations

Other articles from this issue

Recommanded Articles

상단으로 이동

Journal of Korean Medicine